LIC2A_HAEIN
ID LIC2A_HAEIN Reviewed; 302 AA.
AC Q03974; Q48209;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 1.
DT 03-AUG-2022, entry version 108.
DE RecName: Full=Lipooligosaccharide biosynthesis protein lex-1;
DE EC=2.-.-.-;
GN Name=lex1; Synonyms=lic2A; OrderedLocusNames=HI_0550;
OS Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales;
OC Pasteurellaceae; Haemophilus.
OX NCBI_TaxID=71421;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 51907 / DSM 11121 / KW20 / Rd;
RX PubMed=7542800; DOI=10.1126/science.7542800;
RA Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F.,
RA Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M.,
RA McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D.,
RA Scott J.D., Shirley R., Liu L.-I., Glodek A., Kelley J.M., Weidman J.F.,
RA Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R.,
RA Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D.,
RA Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A.,
RA Small K.V., Fraser C.M., Smith H.O., Venter J.C.;
RT "Whole-genome random sequencing and assembly of Haemophilus influenzae
RT Rd.";
RL Science 269:496-512(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=DL42 / Serotype B;
RX PubMed=1956289; DOI=10.1111/j.1365-2958.1991.tb01884.x;
RA Cope L.D., Yogev R., Mertsola J., Latimer J.L., Hanson M.S.,
RA McCracken G.H. Jr., Hansen E.J.;
RT "Molecular cloning of a gene involved in lipooligosaccharide biosynthesis
RT and virulence expression by Haemophilus influenzae type B.";
RL Mol. Microbiol. 5:1113-1124(1991).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=RM 7004 / Serotype B;
RX PubMed=7523834; DOI=10.1111/j.1365-2958.1993.tb01257.x;
RA High N.J., Deadman M.E., Moxon E.R.;
RT "The role of a repetitive DNA motif (5'-CAAT-3') in the variable expression
RT of the Haemophilus influenzae lipopolysaccharide epitope alpha Gal(1-4)beta
RT Gal.";
RL Mol. Microbiol. 9:1275-1282(1993).
RN [4]
RP MUTAGENESIS OF THE SINQ REPEATS, AND EXTENT OF VARIATION IN REPEAT NUMBERS.
RC STRAIN=26 strains, and RM 7004 / Serotype B;
RX PubMed=8861214; DOI=10.1111/j.1365-2958.1996.tb02498.x;
RA High N.J., Jennings M.P., Moxon E.R.;
RT "Tandem repeats of the tetramer 5'-CAAT-3' present in lic2A are required
RT for phase variation but not lipopolysaccharide biosynthesis in Haemophilus
RT influenzae.";
RL Mol. Microbiol. 20:165-174(1996).
CC -!- FUNCTION: Involved in extracellular lipooligosaccharide (LOS)
CC biosynthesis and virulence expression. Involved in the synthesis of the
CC oligosaccharide moiety of the LOS molecule by adding GalNAc.
CC -!- DOMAIN: Between 2 and 7 copies of the SINQ repeats are to be found in
CC different strains; these are thought to serve to generate phase-
CC variable expression of this gene. The (SINQ)n peptide may form a random
CC coiled structure that permits appropriate interactions between the
CC N- and C-terminal domains of the protein.
CC -!- SIMILARITY: Belongs to the glycosyltransferase 25 family.
CC {ECO:0000305}.
CC -!- CAUTION: It is uncertain whether Met-1 or Met-11 is the initiator.
CC {ECO:0000305}.
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DR EMBL; L42023; AAC22208.1; -; Genomic_DNA.
DR EMBL; X56903; CAA40221.1; -; Genomic_DNA.
DR EMBL; L19441; AAA65534.1; -; Genomic_DNA.
DR PIR; A64077; A64077.
DR RefSeq; NP_438708.1; NC_000907.1.
DR RefSeq; WP_005694147.1; NC_000907.1.
DR AlphaFoldDB; Q03974; -.
DR STRING; 71421.HI_0550; -.
DR CAZy; GT25; Glycosyltransferase Family 25.
DR PRIDE; Q03974; -.
DR EnsemblBacteria; AAC22208; AAC22208; HI_0550.
DR KEGG; hin:HI_0550; -.
DR PATRIC; fig|71421.8.peg.570; -.
DR eggNOG; COG3306; Bacteria.
DR HOGENOM; CLU_071269_2_0_6; -.
DR OMA; KEIVRFM; -.
DR PhylomeDB; Q03974; -.
DR BioCyc; HINF71421:G1GJ1-563-MON; -.
DR Proteomes; UP000000579; Chromosome.
DR GO; GO:0016757; F:glycosyltransferase activity; IEA:UniProtKB-KW.
DR CDD; cd06532; Glyco_transf_25; 1.
DR InterPro; IPR002654; Glyco_trans_25.
DR Pfam; PF01755; Glyco_transf_25; 1.
PE 1: Evidence at protein level;
KW Glycosyltransferase; Reference proteome; Repeat; Transferase; Virulence.
FT CHAIN 1..302
FT /note="Lipooligosaccharide biosynthesis protein lex-1"
FT /id="PRO_0000216233"
FT REPEAT 42..45
FT /note="1"
FT /evidence="ECO:0000269|PubMed:8861214"
FT REPEAT 46..49
FT /note="2"
FT /evidence="ECO:0000269|PubMed:8861214"
FT REPEAT 50..53
FT /note="3"
FT /evidence="ECO:0000269|PubMed:8861214"
FT REPEAT 54..57
FT /note="4"
FT /evidence="ECO:0000269|PubMed:8861214"
FT REPEAT 58..61
FT /note="5"
FT /evidence="ECO:0000269|PubMed:8861214"
FT REPEAT 62..65
FT /note="6"
FT /evidence="ECO:0000269|PubMed:8861214"
FT REPEAT 66..69
FT /note="7"
FT /evidence="ECO:0000269|PubMed:8861214"
FT REGION 42..69
FT /note="7 X 4 AA tandem repeats of S-I-N-Q"
FT VARIANT 29
FT /note="K -> N (in strain: DL42 and RM 7004)"
FT VARIANT 63..70
FT /note="Missing (in strain: RM 7004)"
FT VARIANT 67..70
FT /note="Missing (in strain: DL42)"
FT VARIANT 106
FT /note="F -> L (in strain: DL42 and RM 7004)"
FT VARIANT 151
FT /note="Q -> R (in strain: DL42 and RM 7004)"
FT VARIANT 256
FT /note="S -> P (in strain: RM 7004)"
FT VARIANT 274
FT /note="N -> D (in strain: RM 7004)"
FT MUTAGEN 41..71
FT /note="Missing: Reduces the rate of phase variation of the
FT LOS, but does not abolish it."
FT /evidence="ECO:0000269|PubMed:8861214"
SQ SEQUENCE 302 AA; 35490 MW; BFC204F9B3372D2C CRC64;
MSAIENIVIS MENATERRKH ITKQFESKKL SFSFFNAYTY QSINQSINQS INQSINQSIN
QSINQSINQS NSILHNIEES RILTKGEKGC LISHFLLWNK CVNENFEYLK IFEDDVILGE
NAEVFLNQNE WLKTRFDFND IFIIRLETFL QPVKLEKQTK IPPFNSRNFD ILKSTHWGTA
GYIISQGAAK YVIEYLKNIP SDEIVAVDEL IFNKLVDVDN YIVYQLNPAI CIQELQANQS
KSVLTSGLEK ERQKRSKIRK KKTLKQRLTR IKENIIRALN RKKWKEQQRI KEMQGKEIVR
FM
