ID   LIGB_CITK8              Reviewed;         560 AA.
AC   A8ARN4;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   15-JAN-2008, sequence version 2.
DT   03-AUG-2022, entry version 73.
DE   RecName: Full=DNA ligase B {ECO:0000255|HAMAP-Rule:MF_01587};
DE            EC=6.5.1.2 {ECO:0000255|HAMAP-Rule:MF_01587};
DE   AltName: Full=Polydeoxyribonucleotide synthase [NAD(+)] B {ECO:0000255|HAMAP-Rule:MF_01587};
GN   Name=ligB {ECO:0000255|HAMAP-Rule:MF_01587}; OrderedLocusNames=CKO_05104;
OS   Citrobacter koseri (strain ATCC BAA-895 / CDC 4225-83 / SGSC4696).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Citrobacter.
OX   NCBI_TaxID=290338;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-895 / CDC 4225-83 / SGSC4696;
RG   The Citrobacter koseri Genome Sequencing Project;
RA   McClelland M., Sanderson E.K., Porwollik S., Spieth J., Clifton W.S.,
RA   Latreille P., Courtney L., Wang C., Pepin K., Bhonagiri V., Nash W.,
RA   Johnson M., Thiruvilangam P., Wilson R.;
RL   Submitted (AUG-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the formation of phosphodiester linkages between
CC       5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD
CC       as a coenzyme and as the energy source for the reaction.
CC       {ECO:0000255|HAMAP-Rule:MF_01587}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=NAD(+) + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-
CC         (deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + beta-
CC         nicotinamide D-nucleotide.; EC=6.5.1.2; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_01587};
CC   -!- SIMILARITY: Belongs to the NAD-dependent DNA ligase family. LigB
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_01587}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=ABV16147.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; CP000822; ABV16147.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; WP_024131064.1; NC_009792.1.
DR   AlphaFoldDB; A8ARN4; -.
DR   SMR; A8ARN4; -.
DR   STRING; 290338.CKO_05104; -.
DR   EnsemblBacteria; ABV16147; ABV16147; CKO_05104.
DR   GeneID; 45138556; -.
DR   KEGG; cko:CKO_05104; -.
DR   HOGENOM; CLU_489786_0_0_6; -.
DR   OMA; DLWIQPK; -.
DR   OrthoDB; 241401at2; -.
DR   Proteomes; UP000008148; Chromosome.
DR   GO; GO:0003911; F:DNA ligase (NAD+) activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   Gene3D; 2.40.50.140; -; 1.
DR   HAMAP; MF_01587; DNA_ligase_B; 1.
DR   InterPro; IPR018239; DNA_ligase_AS.
DR   InterPro; IPR020923; DNA_ligase_B.
DR   InterPro; IPR033136; DNA_ligase_CS.
DR   InterPro; IPR013839; DNAligase_adenylation.
DR   InterPro; IPR013840; DNAligase_N.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR004150; NAD_DNA_ligase_OB.
DR   InterPro; IPR010994; RuvA_2-like.
DR   Pfam; PF01653; DNA_ligase_aden; 1.
DR   Pfam; PF03120; DNA_ligase_OB; 1.
DR   SMART; SM00532; LIGANc; 1.
DR   SUPFAM; SSF47781; SSF47781; 1.
DR   SUPFAM; SSF50249; SSF50249; 1.
DR   PROSITE; PS01055; DNA_LIGASE_N1; 1.
DR   PROSITE; PS01056; DNA_LIGASE_N2; 1.
PE   3: Inferred from homology;
KW   DNA damage; DNA repair; DNA replication; Ligase; NAD; Reference proteome.
FT   CHAIN           1..560
FT                   /note="DNA ligase B"
FT                   /id="PRO_0000313534"
FT   ACT_SITE        124
FT                   /note="N6-AMP-lysine intermediate"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01587"
SQ   SEQUENCE   560 AA;  62937 MW;  C6E955FF977041F7 CRC64;
     MRRGKSIVLS LLLWHFPVWA ACPDWSPARA NDEIARLQQQ LAQWNDSYWQ QGVSAVDDSV
     YDQLSAQLVQ WQRCFGGDTG AELAPPVSGS LPHPVPHTGV RKLADKQAVQ QWMRKRSDLW
     VQPKVDGVAV TLVYRDGRLV SAISRGNGLK GEDWTQKVRL IPAVPQSTKG ALANSTLQGE
     IFLLHDNHIQ RQMGGMNARS KVAGMMMRQN NTSSLQSLSV FIWAWPDGPA TMPERLRQLS
     GAGFGFAQQY SQPVKSADDV ERVRTAWWTT GLPFVTDGVV VRTGKEPEAR HWMPGQGDWL
     VAWKYPPVAK VAEVTGVQFA VGKSGKISVV ASLAPVMLDD KRVKRVNVGS VRRWEEWDIA
     PGDHILVSLA GQGIPRIDKV VWRSTQRDKP APPGNRYNAL TCFYATEVCQ EQFIARLVWL
     GSKEALEVDG MGEAGWRVLH QAHRFEHIFS WLALTQEQLQ ATPGFSKEKS KQLWHQFNLV
     RHRPFIRWVM AMGIPLTQGA LKANGDRSWG QLLARTAEYW QQLPTTGEGR ARRVIQWRDN
     PEIRALGNWL AARHINGFSP