ID   LIGB_PECCP              Reviewed;         562 AA.
AC   C6DJC3;
DT   22-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-SEP-2009, sequence version 1.
DT   03-AUG-2022, entry version 66.
DE   RecName: Full=DNA ligase B {ECO:0000255|HAMAP-Rule:MF_01587};
DE            EC=6.5.1.2 {ECO:0000255|HAMAP-Rule:MF_01587};
DE   AltName: Full=Polydeoxyribonucleotide synthase [NAD(+)] B {ECO:0000255|HAMAP-Rule:MF_01587};
GN   Name=ligB {ECO:0000255|HAMAP-Rule:MF_01587}; OrderedLocusNames=PC1_4208;
OS   Pectobacterium carotovorum subsp. carotovorum (strain PC1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Pectobacteriaceae; Pectobacterium.
OX   NCBI_TaxID=561230;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=PC1;
RG   US DOE Joint Genome Institute;
RA   Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Tice H., Bruce D.,
RA   Goodwin L., Pitluck S., Munk A.C., Brettin T., Detter J.C., Han C.,
RA   Tapia R., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N.,
RA   Balakrishnan V., Glasner J., Perna N.T.;
RT   "Complete sequence of Pectobacterium carotovorum subsp. carotovorum PC1.";
RL   Submitted (JUL-2009) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the formation of phosphodiester linkages between
CC       5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD
CC       as a coenzyme and as the energy source for the reaction.
CC       {ECO:0000255|HAMAP-Rule:MF_01587}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=NAD(+) + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-
CC         (deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + beta-
CC         nicotinamide D-nucleotide.; EC=6.5.1.2; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_01587};
CC   -!- SIMILARITY: Belongs to the NAD-dependent DNA ligase family. LigB
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_01587}.
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DR   EMBL; CP001657; ACT15222.1; -; Genomic_DNA.
DR   RefSeq; WP_015842291.1; NC_012917.1.
DR   AlphaFoldDB; C6DJC3; -.
DR   SMR; C6DJC3; -.
DR   STRING; 561230.PC1_4208; -.
DR   EnsemblBacteria; ACT15222; ACT15222; PC1_4208.
DR   KEGG; pct:PC1_4208; -.
DR   eggNOG; COG0272; Bacteria.
DR   HOGENOM; CLU_489786_0_0_6; -.
DR   OMA; DLWIQPK; -.
DR   OrthoDB; 241401at2; -.
DR   Proteomes; UP000002736; Chromosome.
DR   GO; GO:0003911; F:DNA ligase (NAD+) activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   Gene3D; 2.40.50.140; -; 1.
DR   HAMAP; MF_01587; DNA_ligase_B; 1.
DR   InterPro; IPR018239; DNA_ligase_AS.
DR   InterPro; IPR020923; DNA_ligase_B.
DR   InterPro; IPR001679; DNAligase.
DR   InterPro; IPR013839; DNAligase_adenylation.
DR   InterPro; IPR013840; DNAligase_N.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR004150; NAD_DNA_ligase_OB.
DR   InterPro; IPR010994; RuvA_2-like.
DR   Pfam; PF01653; DNA_ligase_aden; 1.
DR   Pfam; PF03120; DNA_ligase_OB; 1.
DR   PIRSF; PIRSF001604; LigA; 1.
DR   SMART; SM00532; LIGANc; 1.
DR   SUPFAM; SSF47781; SSF47781; 1.
DR   SUPFAM; SSF50249; SSF50249; 1.
DR   PROSITE; PS01055; DNA_LIGASE_N1; 1.
PE   3: Inferred from homology;
KW   DNA damage; DNA repair; DNA replication; Ligase; NAD.
FT   CHAIN           1..562
FT                   /note="DNA ligase B"
FT                   /id="PRO_1000215633"
FT   ACT_SITE        128
FT                   /note="N6-AMP-lysine intermediate"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01587"
SQ   SEQUENCE   562 AA;  63997 MW;  533CAC2C1C748BB6 CRC64;
     MWLRVCTFLI IIVGGISGFS AVAGVCPDWD NTRSKKEIDA LRQQLEQWDD VYYTEGKSPV
     ADDVYDQLRE QLNQWLGCFQ PQSVASVRLP DHGKQLHPVA HTGLKKLSDR EQLVQWIAQR
     EDVWIQPKID GVAVTLVYQH GKLESAVSRG NGLQGEDWTE KVRLIPEIPH LLTNVPSLLV
     LQGELFLKMT DHRQHIQGGV NARSVVAGEM RRHQPSPVLS QIGLFIWEWP DGPKTMPERL
     EKLTEMGFAM TADYTHAIGS FADAEKWHHY WYHNPLPFVT DGVVLRQTKE PQGRYWRNTS
     ADWAIAWKYP PFHQVAEVID VVFSVGRTGK LAVVLKLNLL KLDDKSVSRV NVGSLSRWKQ
     WDVLPGDRVS VSLAGQGIPR LDNVVWRSTE RLPIVSPSEH DFHAFSCFRY STVCQQQFLA
     RLVWLSGEHG LNLTGVRDGM WLRLMQHGLL EDVLSWLSLT EAQLNAVTGM GDKRARDIYK
     SLQSARQMPL SRWLLALGLP VPRSAISALN HVNWLALQQW TAQQWRQFSG IGERRAEEIM
     AFLKHPAVVE FIARLDAEGI HR