ID   LIGB_PHOLL              Reviewed;         580 AA.
AC   Q7N9N4;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   15-DEC-2003, sequence version 1.
DT   03-AUG-2022, entry version 108.
DE   RecName: Full=DNA ligase B {ECO:0000255|HAMAP-Rule:MF_01587};
DE            EC=6.5.1.2 {ECO:0000255|HAMAP-Rule:MF_01587};
DE   AltName: Full=Polydeoxyribonucleotide synthase [NAD(+)] B {ECO:0000255|HAMAP-Rule:MF_01587};
GN   Name=ligB {ECO:0000255|HAMAP-Rule:MF_01587}; OrderedLocusNames=plu0286;
OS   Photorhabdus laumondii subsp. laumondii (strain DSM 15139 / CIP 105565 /
OS   TT01).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Morganellaceae; Photorhabdus.
OX   NCBI_TaxID=243265;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 15139 / CIP 105565 / TT01;
RX   PubMed=14528314; DOI=10.1038/nbt886;
RA   Duchaud E., Rusniok C., Frangeul L., Buchrieser C., Givaudan A.,
RA   Taourit S., Bocs S., Boursaux-Eude C., Chandler M., Charles J.-F.,
RA   Dassa E., Derose R., Derzelle S., Freyssinet G., Gaudriault S., Medigue C.,
RA   Lanois A., Powell K., Siguier P., Vincent R., Wingate V., Zouine M.,
RA   Glaser P., Boemare N., Danchin A., Kunst F.;
RT   "The genome sequence of the entomopathogenic bacterium Photorhabdus
RT   luminescens.";
RL   Nat. Biotechnol. 21:1307-1313(2003).
CC   -!- FUNCTION: Catalyzes the formation of phosphodiester linkages between
CC       5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD
CC       as a coenzyme and as the energy source for the reaction.
CC       {ECO:0000255|HAMAP-Rule:MF_01587}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=NAD(+) + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-
CC         (deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + beta-
CC         nicotinamide D-nucleotide.; EC=6.5.1.2; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_01587};
CC   -!- SIMILARITY: Belongs to the NAD-dependent DNA ligase family. LigB
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_01587}.
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DR   EMBL; BX571859; CAE12581.1; -; Genomic_DNA.
DR   RefSeq; WP_011144684.1; NC_005126.1.
DR   AlphaFoldDB; Q7N9N4; -.
DR   SMR; Q7N9N4; -.
DR   STRING; 243265.plu0286; -.
DR   EnsemblBacteria; CAE12581; CAE12581; plu0286.
DR   GeneID; 24168365; -.
DR   KEGG; plu:plu0286; -.
DR   eggNOG; COG0272; Bacteria.
DR   HOGENOM; CLU_489786_0_0_6; -.
DR   OMA; DLWIQPK; -.
DR   OrthoDB; 241401at2; -.
DR   BioCyc; PLUM243265:PLU_RS01395-MON; -.
DR   Proteomes; UP000002514; Chromosome.
DR   GO; GO:0003911; F:DNA ligase (NAD+) activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   Gene3D; 2.40.50.140; -; 1.
DR   HAMAP; MF_01587; DNA_ligase_B; 1.
DR   InterPro; IPR020923; DNA_ligase_B.
DR   InterPro; IPR013839; DNAligase_adenylation.
DR   InterPro; IPR013840; DNAligase_N.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR004150; NAD_DNA_ligase_OB.
DR   InterPro; IPR010994; RuvA_2-like.
DR   Pfam; PF01653; DNA_ligase_aden; 1.
DR   Pfam; PF03120; DNA_ligase_OB; 1.
DR   SMART; SM00532; LIGANc; 1.
DR   SUPFAM; SSF47781; SSF47781; 1.
DR   SUPFAM; SSF50249; SSF50249; 1.
PE   3: Inferred from homology;
KW   DNA damage; DNA repair; DNA replication; Ligase; NAD; Reference proteome.
FT   CHAIN           1..580
FT                   /note="DNA ligase B"
FT                   /id="PRO_0000313543"
FT   ACT_SITE        135
FT                   /note="N6-AMP-lysine intermediate"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01587"
SQ   SEQUENCE   580 AA;  65926 MW;  CF33B2C6359093B7 CRC64;
     MLNLLILILF IVFSSPIVAA EIFVSNTEKN CPEWSQDKLQ RETNSLMQQM AHWDQLYRQK
     GISEIDDEVY DQLMSELTHW QFCLKQAPHS DFPVEVFPDN KLVHPVAHTG LSKLKDKQEI
     NRWLHGRLPV WLQPKIDGVA VTLVYQNGKL VSLISRGNGA EGVNWTAKSR YIPDIPQNIE
     NAPPDLVLQG ELFLHKEEHR QQLLGSDGAR NRVAGLMMRK DHSSELKQIG LFIWSWPDGP
     AEMESKLRKL ADMGFPLALR YSHKIEEPEQ VQKLRMHYFE QPMLFATDGI VLKQEIEPKG
     NQWRSGSNSW AVAWKHPLRH QVTQVREVNF TIGRTGKISV VLGLDKVKLD DRQISRVNIG
     SVRRWRQLDV LPGDRVTLTL AGHGIPKLAQ VVWRIKERQD IQPPDKQHYH ALSCLTLTVG
     CEQQFNARLK WLGENLQMTG VSSGSWAMLT ERKLVTDLTG WLALSAQQIA ALPGIGDKRA
     QAIYSQFSKA KNQPFSYWMK GIGVPYMDKL SDNVYHWQQL EQKLAAAQLK QQLTVGQLKK
     LSDFVSDAQI KAIAEKLSVA GITGFDRENG VVVNPADMAQ