ID   LIGB_PSEE4              Reviewed;         557 AA.
AC   Q1I3X5;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   13-JUN-2006, sequence version 1.
DT   03-AUG-2022, entry version 86.
DE   RecName: Full=DNA ligase B {ECO:0000255|HAMAP-Rule:MF_01587};
DE            EC=6.5.1.2 {ECO:0000255|HAMAP-Rule:MF_01587};
DE   AltName: Full=Polydeoxyribonucleotide synthase [NAD(+)] B {ECO:0000255|HAMAP-Rule:MF_01587};
GN   Name=ligB {ECO:0000255|HAMAP-Rule:MF_01587}; OrderedLocusNames=PSEEN5027;
OS   Pseudomonas entomophila (strain L48).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=384676;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=L48;
RX   PubMed=16699499; DOI=10.1038/nbt1212;
RA   Vodovar N., Vallenet D., Cruveiller S., Rouy Z., Barbe V., Acosta C.,
RA   Cattolico L., Jubin C., Lajus A., Segurens B., Vacherie B., Wincker P.,
RA   Weissenbach J., Lemaitre B., Medigue C., Boccard F.;
RT   "Complete genome sequence of the entomopathogenic and metabolically
RT   versatile soil bacterium Pseudomonas entomophila.";
RL   Nat. Biotechnol. 24:673-679(2006).
CC   -!- FUNCTION: Catalyzes the formation of phosphodiester linkages between
CC       5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD
CC       as a coenzyme and as the energy source for the reaction.
CC       {ECO:0000255|HAMAP-Rule:MF_01587}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=NAD(+) + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-
CC         (deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + beta-
CC         nicotinamide D-nucleotide.; EC=6.5.1.2; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_01587};
CC   -!- SIMILARITY: Belongs to the NAD-dependent DNA ligase family. LigB
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_01587}.
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DR   EMBL; CT573326; CAK17661.1; -; Genomic_DNA.
DR   RefSeq; WP_011536021.1; NC_008027.1.
DR   AlphaFoldDB; Q1I3X5; -.
DR   SMR; Q1I3X5; -.
DR   STRING; 384676.PSEEN5027; -.
DR   EnsemblBacteria; CAK17661; CAK17661; PSEEN5027.
DR   KEGG; pen:PSEEN5027; -.
DR   eggNOG; COG0272; Bacteria.
DR   HOGENOM; CLU_489786_0_0_6; -.
DR   OMA; DLWIQPK; -.
DR   OrthoDB; 241401at2; -.
DR   Proteomes; UP000000658; Chromosome.
DR   GO; GO:0003911; F:DNA ligase (NAD+) activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   Gene3D; 2.40.50.140; -; 1.
DR   HAMAP; MF_01587; DNA_ligase_B; 1.
DR   InterPro; IPR020923; DNA_ligase_B.
DR   InterPro; IPR033136; DNA_ligase_CS.
DR   InterPro; IPR013839; DNAligase_adenylation.
DR   InterPro; IPR013840; DNAligase_N.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR004150; NAD_DNA_ligase_OB.
DR   InterPro; IPR010994; RuvA_2-like.
DR   Pfam; PF01653; DNA_ligase_aden; 1.
DR   Pfam; PF03120; DNA_ligase_OB; 1.
DR   SMART; SM00532; LIGANc; 1.
DR   SUPFAM; SSF47781; SSF47781; 1.
DR   SUPFAM; SSF50249; SSF50249; 1.
DR   PROSITE; PS01056; DNA_LIGASE_N2; 1.
PE   3: Inferred from homology;
KW   DNA damage; DNA repair; DNA replication; Ligase; NAD.
FT   CHAIN           1..557
FT                   /note="DNA ligase B"
FT                   /id="PRO_0000313544"
FT   ACT_SITE        125
FT                   /note="N6-AMP-lysine intermediate"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01587"
SQ   SEQUENCE   557 AA;  62040 MW;  458D3A3C3726023C CRC64;
     MSLRLLIPLL FLPGLALADH RPCPDWPAQR ARAEVSQLLK TLSHWDDHYH RQGVALVADE
     LYDQSRQHLR HLQGCFDLAS NDAPLATARG QVPHPVPHTG VDKLRDEPAV QRWLQGRQGM
     WIQPKVDGVA VSLVYQQGQL TQLLSRGDGV LGHDWSRHIP QLGRIVRQLP QPLDTVFQGE
     LYWRLADHVQ AEAGSANARG IVAGLLARKQ LSDEQGSGIG LFVWDWPAGP GSQAERLAQL
     TALGFIDSQR FSVAIEGFDA AAHWRQHWYR TALPFATDGV ILRQDARPPA QRWRAQAPYW
     IAAWKYPFSQ ALAQVRDIHF RIGRTGRITP LLQLEPIQLD DRRISQVSLG SLARWTQLDI
     RPGDQVAVSL AGLTIPRVES VVHRSPLRAP VLVPDPADHH LLSCWQASPA CQEQFLARLA
     WLGGNKGLDM AGVGSGVWHQ LVESGKVATL SDWLELTRED LLEVPGIAQA RAERLLQAFS
     LGRRQPFERW LRGLGLPAPS HFSPGADWSA LASRNIEQWL AEPGVGAVRA AQLQAFLSHE
     QVQALARRLR AHEIEGF