ID   LIGB_PSEF5              Reviewed;         556 AA.
AC   Q4K4I6;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   02-AUG-2005, sequence version 1.
DT   03-AUG-2022, entry version 91.
DE   RecName: Full=DNA ligase B {ECO:0000255|HAMAP-Rule:MF_01587};
DE            EC=6.5.1.2 {ECO:0000255|HAMAP-Rule:MF_01587};
DE   AltName: Full=Polydeoxyribonucleotide synthase [NAD(+)] B {ECO:0000255|HAMAP-Rule:MF_01587};
GN   Name=ligB {ECO:0000255|HAMAP-Rule:MF_01587}; OrderedLocusNames=PFL_5789;
OS   Pseudomonas fluorescens (strain ATCC BAA-477 / NRRL B-23932 / Pf-5).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=220664;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-477 / NRRL B-23932 / Pf-5;
RX   PubMed=15980861; DOI=10.1038/nbt1110;
RA   Paulsen I.T., Press C.M., Ravel J., Kobayashi D.Y., Myers G.S.A.,
RA   Mavrodi D.V., DeBoy R.T., Seshadri R., Ren Q., Madupu R., Dodson R.J.,
RA   Durkin A.S., Brinkac L.M., Daugherty S.C., Sullivan S.A., Rosovitz M.J.,
RA   Gwinn M.L., Zhou L., Schneider D.J., Cartinhour S.W., Nelson W.C.,
RA   Weidman J., Watkins K., Tran K., Khouri H., Pierson E.A., Pierson L.S. III,
RA   Thomashow L.S., Loper J.E.;
RT   "Complete genome sequence of the plant commensal Pseudomonas fluorescens
RT   Pf-5.";
RL   Nat. Biotechnol. 23:873-878(2005).
CC   -!- FUNCTION: Catalyzes the formation of phosphodiester linkages between
CC       5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD
CC       as a coenzyme and as the energy source for the reaction.
CC       {ECO:0000255|HAMAP-Rule:MF_01587}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=NAD(+) + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-
CC         (deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + beta-
CC         nicotinamide D-nucleotide.; EC=6.5.1.2; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_01587};
CC   -!- SIMILARITY: Belongs to the NAD-dependent DNA ligase family. LigB
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_01587}.
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DR   EMBL; CP000076; AAY94979.1; -; Genomic_DNA.
DR   RefSeq; WP_011063963.1; NC_004129.6.
DR   AlphaFoldDB; Q4K4I6; -.
DR   SMR; Q4K4I6; -.
DR   STRING; 220664.PFL_5789; -.
DR   EnsemblBacteria; AAY94979; AAY94979; PFL_5789.
DR   KEGG; pfl:PFL_5789; -.
DR   PATRIC; fig|220664.5.peg.5902; -.
DR   eggNOG; COG0272; Bacteria.
DR   HOGENOM; CLU_489786_0_0_6; -.
DR   OMA; DLWIQPK; -.
DR   OrthoDB; 241401at2; -.
DR   Proteomes; UP000008540; Chromosome.
DR   GO; GO:0003911; F:DNA ligase (NAD+) activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   Gene3D; 2.40.50.140; -; 1.
DR   HAMAP; MF_01587; DNA_ligase_B; 1.
DR   InterPro; IPR020923; DNA_ligase_B.
DR   InterPro; IPR001679; DNAligase.
DR   InterPro; IPR013839; DNAligase_adenylation.
DR   InterPro; IPR013840; DNAligase_N.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR004150; NAD_DNA_ligase_OB.
DR   InterPro; IPR010994; RuvA_2-like.
DR   Pfam; PF01653; DNA_ligase_aden; 1.
DR   Pfam; PF03120; DNA_ligase_OB; 1.
DR   PIRSF; PIRSF001604; LigA; 1.
DR   SMART; SM00532; LIGANc; 1.
DR   SUPFAM; SSF47781; SSF47781; 1.
DR   SUPFAM; SSF50249; SSF50249; 1.
PE   3: Inferred from homology;
KW   DNA damage; DNA repair; DNA replication; Ligase; NAD; Reference proteome.
FT   CHAIN           1..556
FT                   /note="DNA ligase B"
FT                   /id="PRO_0000313545"
FT   ACT_SITE        124
FT                   /note="N6-AMP-lysine intermediate"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01587"
SQ   SEQUENCE   556 AA;  62421 MW;  E0DD7935442246A7 CRC64;
     MLPRAVVLLS LTPFFAQAAC PDWPVPRATD EITALQQQLQ RWDSSYYRDG RSLVADELYD
     QARQRLLTWR QCFPQAPRAM DQPLLGAGGS IRHPIPHTGV EKLADANAVT GWLKGRDDIW
     AQPKVDGVAV TLIYHRGQFQ QALSRGDGVH GHDWTRAAWQ IEAIPKRLPR PVDLLLQGEL
     YLRLEQHVQA RSGSLKARNR VAGWMARKQL TAAEAAQIGL FVWDWPQGPA TLSERLEQLA
     DLGFAEPREY SRPVGGFADA QQWRARWYRS PLPFASDGII LRQSRRPAAE RWQAKAPYWI
     AAWKYPYAQV LASVRQVDFR IGRTGRITPL LEIEPVTLDD RQIRRVSLGS LQRWRSLDIV
     PGDQVAISLA GLSIPRLDEV VLRSTLRQSI QPPREADFHF LSCWQPSPGC EEQFLARLTW
     LSSKQGLNLQ NIGSRTWARL VETGRIKGLL DWLTLDQAEL ANIAGFGDRS SKRLLESLHS
     ARQQPFPRWL KALGIPPSGS ADLSGPWQAL AMKNSLDWQN EAGIGAERAA QLVAFFRAPE
     VLALQGALNS AGIEGF