ID   LIGB_PSEFS              Reviewed;         554 AA.
AC   C3K3F7;
DT   01-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT   16-JUN-2009, sequence version 1.
DT   03-AUG-2022, entry version 84.
DE   RecName: Full=DNA ligase B {ECO:0000255|HAMAP-Rule:MF_01587};
DE            EC=6.5.1.2 {ECO:0000255|HAMAP-Rule:MF_01587};
DE   AltName: Full=Polydeoxyribonucleotide synthase [NAD(+)] B {ECO:0000255|HAMAP-Rule:MF_01587};
GN   Name=ligB {ECO:0000255|HAMAP-Rule:MF_01587}; OrderedLocusNames=PFLU_5711;
OS   Pseudomonas fluorescens (strain SBW25).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=216595;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=SBW25;
RX   PubMed=19432983; DOI=10.1186/gb-2009-10-5-r51;
RA   Silby M.W., Cerdeno-Tarraga A.M., Vernikos G.S., Giddens S.R.,
RA   Jackson R.W., Preston G.M., Zhang X.-X., Moon C.D., Gehrig S.M.,
RA   Godfrey S.A.C., Knight C.G., Malone J.G., Robinson Z., Spiers A.J.,
RA   Harris S., Challis G.L., Yaxley A.M., Harris D., Seeger K., Murphy L.,
RA   Rutter S., Squares R., Quail M.A., Saunders E., Mavromatis K.,
RA   Brettin T.S., Bentley S.D., Hothersall J., Stephens E., Thomas C.M.,
RA   Parkhill J., Levy S.B., Rainey P.B., Thomson N.R.;
RT   "Genomic and genetic analyses of diversity and plant interactions of
RT   Pseudomonas fluorescens.";
RL   Genome Biol. 10:R51.1-R51.16(2009).
CC   -!- FUNCTION: Catalyzes the formation of phosphodiester linkages between
CC       5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD
CC       as a coenzyme and as the energy source for the reaction.
CC       {ECO:0000255|HAMAP-Rule:MF_01587}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=NAD(+) + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-
CC         (deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + beta-
CC         nicotinamide D-nucleotide.; EC=6.5.1.2; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_01587};
CC   -!- SIMILARITY: Belongs to the NAD-dependent DNA ligase family. LigB
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_01587}.
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DR   EMBL; AM181176; CAY53062.1; -; Genomic_DNA.
DR   RefSeq; WP_015886299.1; NC_012660.1.
DR   AlphaFoldDB; C3K3F7; -.
DR   SMR; C3K3F7; -.
DR   STRING; 216595.PFLU_5711; -.
DR   EnsemblBacteria; CAY53062; CAY53062; PFLU_5711.
DR   KEGG; pfs:PFLU_5711; -.
DR   PATRIC; fig|216595.4.peg.5834; -.
DR   eggNOG; COG0272; Bacteria.
DR   HOGENOM; CLU_489786_0_0_6; -.
DR   OMA; DLWIQPK; -.
DR   OrthoDB; 241401at2; -.
DR   Proteomes; UP000002332; Chromosome.
DR   GO; GO:0003911; F:DNA ligase (NAD+) activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   Gene3D; 2.40.50.140; -; 1.
DR   HAMAP; MF_01587; DNA_ligase_B; 1.
DR   InterPro; IPR020923; DNA_ligase_B.
DR   InterPro; IPR001679; DNAligase.
DR   InterPro; IPR013839; DNAligase_adenylation.
DR   InterPro; IPR013840; DNAligase_N.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR004150; NAD_DNA_ligase_OB.
DR   InterPro; IPR010994; RuvA_2-like.
DR   Pfam; PF01653; DNA_ligase_aden; 1.
DR   Pfam; PF03120; DNA_ligase_OB; 1.
DR   PIRSF; PIRSF001604; LigA; 1.
DR   SMART; SM00532; LIGANc; 1.
DR   SUPFAM; SSF47781; SSF47781; 1.
DR   SUPFAM; SSF50249; SSF50249; 1.
PE   3: Inferred from homology;
KW   DNA damage; DNA repair; DNA replication; Ligase; NAD; Reference proteome.
FT   CHAIN           1..554
FT                   /note="DNA ligase B"
FT                   /id="PRO_0000381950"
FT   ACT_SITE        122
FT                   /note="N6-AMP-lysine intermediate"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01587"
SQ   SEQUENCE   554 AA;  61594 MW;  C9C0615564CF2D91 CRC64;
     MMHLLFALLL SALPCWVWAQ ACSDEARAHV STLAEQIRQW DDSYHRLGQS PVSDELYDQA
     RQRLAQWHQC FPAPTATPNT PLASSRGAQP HPVAHTGLEK LLDEHAVDAW LGTRKDVWIQ
     PKVDGVAVTL VYQQGRLRQV ISRGDGVMGH DWSASARKIP GIVQQLPDPI DLVLQGELYW
     RLDDHVQSAS GGLNARSKVA GLMNRKHLGD TDAAGIGLFV WAWPQGPAAF TERLSTLKRW
     GFTDTQRFSQ PIRNISEAAH WRAYWYGHPL PFASDGVVLH QAQHAPAERW QVSTPYWAAA
     WKYPTTKALA LVRDVQFKIG RTGRITPMLE LEPVRLDDRQ ISRVSAGSLK RWQSLDIRPG
     DHVSISLAGQ VIPRLDEVIL RSSTRADLPV PNPGKFHALS CWQLDPGCEE QLLARLSWLS
     GNQGLALPHI GRETWSVLIQ AGLIAGFLDW LTLDTAELAN IDGFGDRTRA RVVDSFHSAR
     QRPFAQWLKA LGVPPAARNN LEGDWQTLVA RDTQAWLAID GIGPGRAAQL SAFFRDPHVQ
     ALAETLRVAG IDGF