ID   LIGB_PSEP1              Reviewed;         566 AA.
AC   A5WA01;
DT   01-SEP-2009, integrated into UniProtKB/Swiss-Prot.
DT   10-JUL-2007, sequence version 1.
DT   03-AUG-2022, entry version 74.
DE   RecName: Full=DNA ligase B {ECO:0000255|HAMAP-Rule:MF_01587};
DE            EC=6.5.1.2 {ECO:0000255|HAMAP-Rule:MF_01587};
DE   AltName: Full=Polydeoxyribonucleotide synthase [NAD(+)] B {ECO:0000255|HAMAP-Rule:MF_01587};
GN   Name=ligB {ECO:0000255|HAMAP-Rule:MF_01587}; OrderedLocusNames=Pput_4841;
OS   Pseudomonas putida (strain ATCC 700007 / DSM 6899 / BCRC 17059 / F1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=351746;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700007 / DSM 6899 / BCRC 17059 / F1;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S.,
RA   Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M.,
RA   Hauser L., Kyrpides N., Lykidis A., Parales R., Richardson P.;
RT   "Complete sequence of Pseudomonas putida F1.";
RL   Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the formation of phosphodiester linkages between
CC       5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD
CC       as a coenzyme and as the energy source for the reaction.
CC       {ECO:0000255|HAMAP-Rule:MF_01587}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=NAD(+) + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-
CC         (deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + beta-
CC         nicotinamide D-nucleotide.; EC=6.5.1.2; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_01587};
CC   -!- SIMILARITY: Belongs to the NAD-dependent DNA ligase family. LigB
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_01587}.
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DR   EMBL; CP000712; ABQ80961.1; -; Genomic_DNA.
DR   RefSeq; WP_012053897.1; NC_009512.1.
DR   AlphaFoldDB; A5WA01; -.
DR   SMR; A5WA01; -.
DR   STRING; 351746.Pput_4841; -.
DR   PRIDE; A5WA01; -.
DR   EnsemblBacteria; ABQ80961; ABQ80961; Pput_4841.
DR   KEGG; ppf:Pput_4841; -.
DR   eggNOG; COG0272; Bacteria.
DR   HOGENOM; CLU_489786_0_0_6; -.
DR   OMA; DLWIQPK; -.
DR   OrthoDB; 241401at2; -.
DR   GO; GO:0003911; F:DNA ligase (NAD+) activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   Gene3D; 2.40.50.140; -; 1.
DR   HAMAP; MF_01587; DNA_ligase_B; 1.
DR   InterPro; IPR020923; DNA_ligase_B.
DR   InterPro; IPR033136; DNA_ligase_CS.
DR   InterPro; IPR001679; DNAligase.
DR   InterPro; IPR013839; DNAligase_adenylation.
DR   InterPro; IPR013840; DNAligase_N.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR004150; NAD_DNA_ligase_OB.
DR   InterPro; IPR010994; RuvA_2-like.
DR   Pfam; PF01653; DNA_ligase_aden; 1.
DR   Pfam; PF03120; DNA_ligase_OB; 1.
DR   PIRSF; PIRSF001604; LigA; 1.
DR   SMART; SM00532; LIGANc; 1.
DR   SUPFAM; SSF47781; SSF47781; 1.
DR   SUPFAM; SSF50249; SSF50249; 1.
DR   PROSITE; PS01056; DNA_LIGASE_N2; 1.
PE   3: Inferred from homology;
KW   DNA damage; DNA repair; DNA replication; Ligase; NAD.
FT   CHAIN           1..566
FT                   /note="DNA ligase B"
FT                   /id="PRO_0000381952"
FT   ACT_SITE        125
FT                   /note="N6-AMP-lysine intermediate"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01587"
SQ   SEQUENCE   566 AA;  62595 MW;  AD42049DCEEE75BA CRC64;
     MPYLLLFALL FALNAPLARA ASCPQWSPQQ AKAEVAQLRA TLARWDEHYH RQGIALVADE
     LYDQSRERLN HLQQCFAVGT SPSPLASARG PVPHPVPHTG VDKLADRQAV ARWMAGKSGV
     WVQPKVDGVA VSLTYQQGRL VQLTSRGDGV NGHDWSRHIP QLDAVTRQLP EALDLHLQGE
     LYLRLSDHVQ AKAGSANARG TVAGLLARKQ LTGAQGNAIG LFVWGWPHGP EQQAERLAQL
     ARLGFPDSQL YSIAIDTLED AAHWREHWYR SALPFATDGV ILRQSSRPPA ERWQAKAPYW
     IAAWKYPYAQ ALAEVRDVRF RVGRTGRVTP VLHLLPVTLD DRRITQVSLG SLARWHTLDI
     RPGDQVAISL AGLTIPRLEQ VVHRTVERQA VAAPAPDRYH AHSCWQASEG CDEQFIARLT
     WLGGKQGLAL PRTGPGTWRR LVEAGLVTSM TDWLQLDAER LQQAPGISRL TAAHLLGSFD
     EARSRPFDQW LRALGVPIGK HLPLTGDWQA LASRSAGHWQ TVPGIGAKRS RQLVEFFAAS
     EVQAIAAQLA ETGIEGFRPP PQRIEQ