ID   LIGB_PSEPF              Reviewed;         558 AA.
AC   Q3K5E7;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   08-NOV-2005, sequence version 1.
DT   03-AUG-2022, entry version 96.
DE   RecName: Full=DNA ligase B {ECO:0000255|HAMAP-Rule:MF_01587};
DE            EC=6.5.1.2 {ECO:0000255|HAMAP-Rule:MF_01587};
DE   AltName: Full=Polydeoxyribonucleotide synthase [NAD(+)] B {ECO:0000255|HAMAP-Rule:MF_01587};
GN   Name=ligB {ECO:0000255|HAMAP-Rule:MF_01587}; OrderedLocusNames=Pfl01_5270;
OS   Pseudomonas fluorescens (strain Pf0-1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=205922;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Pf0-1;
RX   PubMed=19432983; DOI=10.1186/gb-2009-10-5-r51;
RA   Silby M.W., Cerdeno-Tarraga A.M., Vernikos G.S., Giddens S.R.,
RA   Jackson R.W., Preston G.M., Zhang X.-X., Moon C.D., Gehrig S.M.,
RA   Godfrey S.A.C., Knight C.G., Malone J.G., Robinson Z., Spiers A.J.,
RA   Harris S., Challis G.L., Yaxley A.M., Harris D., Seeger K., Murphy L.,
RA   Rutter S., Squares R., Quail M.A., Saunders E., Mavromatis K.,
RA   Brettin T.S., Bentley S.D., Hothersall J., Stephens E., Thomas C.M.,
RA   Parkhill J., Levy S.B., Rainey P.B., Thomson N.R.;
RT   "Genomic and genetic analyses of diversity and plant interactions of
RT   Pseudomonas fluorescens.";
RL   Genome Biol. 10:R51.1-R51.16(2009).
CC   -!- FUNCTION: Catalyzes the formation of phosphodiester linkages between
CC       5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD
CC       as a coenzyme and as the energy source for the reaction.
CC       {ECO:0000255|HAMAP-Rule:MF_01587}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=NAD(+) + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-
CC         (deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + beta-
CC         nicotinamide D-nucleotide.; EC=6.5.1.2; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_01587};
CC   -!- SIMILARITY: Belongs to the NAD-dependent DNA ligase family. LigB
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_01587}.
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DR   EMBL; CP000094; ABA77007.1; -; Genomic_DNA.
DR   RefSeq; WP_011336331.1; NC_007492.2.
DR   AlphaFoldDB; Q3K5E7; -.
DR   SMR; Q3K5E7; -.
DR   STRING; 205922.Pfl01_5270; -.
DR   PRIDE; Q3K5E7; -.
DR   EnsemblBacteria; ABA77007; ABA77007; Pfl01_5270.
DR   KEGG; pfo:Pfl01_5270; -.
DR   eggNOG; COG0272; Bacteria.
DR   HOGENOM; CLU_489786_0_0_6; -.
DR   OMA; DLWIQPK; -.
DR   Proteomes; UP000002704; Chromosome.
DR   GO; GO:0003911; F:DNA ligase (NAD+) activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   Gene3D; 2.40.50.140; -; 1.
DR   HAMAP; MF_01587; DNA_ligase_B; 1.
DR   InterPro; IPR020923; DNA_ligase_B.
DR   InterPro; IPR033136; DNA_ligase_CS.
DR   InterPro; IPR001679; DNAligase.
DR   InterPro; IPR013839; DNAligase_adenylation.
DR   InterPro; IPR013840; DNAligase_N.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR004150; NAD_DNA_ligase_OB.
DR   InterPro; IPR010994; RuvA_2-like.
DR   Pfam; PF01653; DNA_ligase_aden; 1.
DR   Pfam; PF03120; DNA_ligase_OB; 1.
DR   PIRSF; PIRSF001604; LigA; 1.
DR   SMART; SM00532; LIGANc; 1.
DR   SUPFAM; SSF47781; SSF47781; 1.
DR   SUPFAM; SSF50249; SSF50249; 1.
DR   PROSITE; PS01056; DNA_LIGASE_N2; 1.
PE   3: Inferred from homology;
KW   DNA damage; DNA repair; DNA replication; Ligase; NAD.
FT   CHAIN           1..558
FT                   /note="DNA ligase B"
FT                   /id="PRO_0000313546"
FT   ACT_SITE        126
FT                   /note="N6-AMP-lysine intermediate"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01587"
SQ   SEQUENCE   558 AA;  62268 MW;  6D905B4E8E6999C1 CRC64;
     MLATLRLFLF FLPFFHLHTF ADDCPDWAAA RASSEVVALQ QQIDRWDDAY HREGRSVIAD
     ELYDQSRLRL NQWRQCFNLP SPPEPLRTAS GPVAHPVAHT GLDKLHDAAD IATWLRDRQN
     VWVQPKVDGV AVTLIYRDGR LHQAISRGDG VRGQDWTASA KKIGAIPQQL TQPQDLLVQG
     ELYWRLNGHM QARAGSANAR ATVAGLLGRK DLGAEHAAGI GLFVWDWPHG PRDLPERITA
     LTQFGFPTTV PYTQAVASLN DAQHWRDHWY HSPLPFATDG VVLRQSLRPP AERWQARPPY
     WAVAWKYPFA QALADVRKVH FKIGRTGRIT PVLELSPVML DDRQIKRVSV SSLRRWQELD
     IRPGDQVAIS LAGLTIPRLD SVVLRSTERA DLNVPLASDF HALSCWQPTP GCESQFLARL
     TWLSGKQGLA LPHVGRGTWE KLLETGRLNS LLDWLTLDGP ELANIAGLGE RSSARLLHSF
     HSARQRPFAQ WLKALGLPPT GQATLADSWQ ALAQRNTEQW QAEAGIGPGR AAQLSAFFRD
     PQVLTLSETL QAAGVDGF