ID   LIGB_PSESM              Reviewed;         561 AA.
AC   Q88AK8;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   03-AUG-2022, entry version 105.
DE   RecName: Full=DNA ligase B {ECO:0000255|HAMAP-Rule:MF_01587};
DE            EC=6.5.1.2 {ECO:0000255|HAMAP-Rule:MF_01587};
DE   AltName: Full=Polydeoxyribonucleotide synthase [NAD(+)] B {ECO:0000255|HAMAP-Rule:MF_01587};
GN   Name=ligB {ECO:0000255|HAMAP-Rule:MF_01587}; OrderedLocusNames=PSPTO_0382;
OS   Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=223283;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-871 / DC3000;
RX   PubMed=12928499; DOI=10.1073/pnas.1731982100;
RA   Buell C.R., Joardar V., Lindeberg M., Selengut J., Paulsen I.T.,
RA   Gwinn M.L., Dodson R.J., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R.,
RA   Daugherty S.C., Brinkac L.M., Beanan M.J., Haft D.H., Nelson W.C.,
RA   Davidsen T.M., Zafar N., Zhou L., Liu J., Yuan Q., Khouri H.M.,
RA   Fedorova N.B., Tran B., Russell D., Berry K.J., Utterback T.R.,
RA   Van Aken S.E., Feldblyum T.V., D'Ascenzo M., Deng W.-L., Ramos A.R.,
RA   Alfano J.R., Cartinhour S., Chatterjee A.K., Delaney T.P., Lazarowitz S.G.,
RA   Martin G.B., Schneider D.J., Tang X., Bender C.L., White O., Fraser C.M.,
RA   Collmer A.;
RT   "The complete genome sequence of the Arabidopsis and tomato pathogen
RT   Pseudomonas syringae pv. tomato DC3000.";
RL   Proc. Natl. Acad. Sci. U.S.A. 100:10181-10186(2003).
CC   -!- FUNCTION: Catalyzes the formation of phosphodiester linkages between
CC       5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD
CC       as a coenzyme and as the energy source for the reaction.
CC       {ECO:0000255|HAMAP-Rule:MF_01587}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=NAD(+) + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-
CC         (deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + beta-
CC         nicotinamide D-nucleotide.; EC=6.5.1.2; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_01587};
CC   -!- SIMILARITY: Belongs to the NAD-dependent DNA ligase family. LigB
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_01587}.
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DR   EMBL; AE016853; AAO53926.1; -; Genomic_DNA.
DR   RefSeq; NP_790231.1; NC_004578.1.
DR   RefSeq; WP_011103125.1; NC_004578.1.
DR   AlphaFoldDB; Q88AK8; -.
DR   SMR; Q88AK8; -.
DR   STRING; 223283.PSPTO_0382; -.
DR   EnsemblBacteria; AAO53926; AAO53926; PSPTO_0382.
DR   GeneID; 1181991; -.
DR   KEGG; pst:PSPTO_0382; -.
DR   PATRIC; fig|223283.9.peg.399; -.
DR   eggNOG; COG0272; Bacteria.
DR   HOGENOM; CLU_489786_0_0_6; -.
DR   OMA; DLWIQPK; -.
DR   OrthoDB; 241401at2; -.
DR   PhylomeDB; Q88AK8; -.
DR   Proteomes; UP000002515; Chromosome.
DR   GO; GO:0003911; F:DNA ligase (NAD+) activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006310; P:DNA recombination; ISS:JCVI.
DR   GO; GO:0006281; P:DNA repair; ISS:JCVI.
DR   GO; GO:0006260; P:DNA replication; ISS:JCVI.
DR   Gene3D; 2.40.50.140; -; 1.
DR   HAMAP; MF_01587; DNA_ligase_B; 1.
DR   InterPro; IPR020923; DNA_ligase_B.
DR   InterPro; IPR001679; DNAligase.
DR   InterPro; IPR013839; DNAligase_adenylation.
DR   InterPro; IPR013840; DNAligase_N.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR004150; NAD_DNA_ligase_OB.
DR   InterPro; IPR010994; RuvA_2-like.
DR   Pfam; PF01653; DNA_ligase_aden; 1.
DR   Pfam; PF03120; DNA_ligase_OB; 1.
DR   PIRSF; PIRSF001604; LigA; 1.
DR   SMART; SM00532; LIGANc; 1.
DR   SUPFAM; SSF47781; SSF47781; 1.
DR   SUPFAM; SSF50249; SSF50249; 1.
PE   3: Inferred from homology;
KW   DNA damage; DNA repair; DNA replication; Ligase; NAD; Reference proteome.
FT   CHAIN           1..561
FT                   /note="DNA ligase B"
FT                   /id="PRO_0000313550"
FT   ACT_SITE        128
FT                   /note="N6-AMP-lysine intermediate"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01587"
SQ   SEQUENCE   561 AA;  62020 MW;  C75EF5A8FB9DA27E CRC64;
     MLPALRLMTC ALLTFFTTLS HASQCPDWSH ARASSEITAL QQQIASWDDS YHRQGLSQIP
     DELYDQSRQK LNDWRSCFAV STPQANPLKT ATGPLAHPVP HTGVNKLADE KDVKAWLKGR
     TDLWIQPKVD GVAVTLVYDE GRLVQAISRG DGTQGQDWTP QARLIKAIPQ QLPQPDSLIL
     QGELYWRLTD HVQAASGSVN ARSKVAGMLA RQTISPQQAD AIGLFVWDWP DGPADMAQRL
     AGLQAMGFNH SVAYTHPLEN HAQAADWREH WYRNPLPFAT DGVIMRQGQR PPAQRWQASA
     PYWIAAWKHP YAQALAEVRK VHFNIGRTGK IAPVLELTPV RLDDRTVSRI SAGSLSRWEK
     LDIRPGDHIA VSLAGLTIPR LDGVVSRAAE RAEMSVPHAS DYHESSCWQA APGCESQFRA
     RLVWLSGKKG LALPGVGPGT WDKLIESGHI SGLLDWMTLN HAELANIPGL AERSSDKLLD
     SLQTARERPF QTWLKAIGLP PAGNAKLPDN WHDLAERSVE QWQAEPGIGP GRAAKLRAFF
     QDPHVQALSQ QLQAQGISGF K