ID   LIGB_PSEU2              Reviewed;         561 AA.
AC   Q4ZLZ9;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   07-JUN-2005, sequence version 1.
DT   03-AUG-2022, entry version 90.
DE   RecName: Full=DNA ligase B {ECO:0000255|HAMAP-Rule:MF_01587};
DE            EC=6.5.1.2 {ECO:0000255|HAMAP-Rule:MF_01587};
DE   AltName: Full=Polydeoxyribonucleotide synthase [NAD(+)] B {ECO:0000255|HAMAP-Rule:MF_01587};
GN   Name=ligB {ECO:0000255|HAMAP-Rule:MF_01587}; OrderedLocusNames=Psyr_4796;
OS   Pseudomonas syringae pv. syringae (strain B728a).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas; Pseudomonas syringae.
OX   NCBI_TaxID=205918;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=B728a;
RX   PubMed=16043691; DOI=10.1073/pnas.0504930102;
RA   Feil H., Feil W.S., Chain P., Larimer F., Dibartolo G., Copeland A.,
RA   Lykidis A., Trong S., Nolan M., Goltsman E., Thiel J., Malfatti S.,
RA   Loper J.E., Lapidus A., Detter J.C., Land M., Richardson P.M.,
RA   Kyrpides N.C., Ivanova N., Lindow S.E.;
RT   "Comparison of the complete genome sequences of Pseudomonas syringae pv.
RT   syringae B728a and pv. tomato DC3000.";
RL   Proc. Natl. Acad. Sci. U.S.A. 102:11064-11069(2005).
CC   -!- FUNCTION: Catalyzes the formation of phosphodiester linkages between
CC       5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD
CC       as a coenzyme and as the energy source for the reaction.
CC       {ECO:0000255|HAMAP-Rule:MF_01587}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=NAD(+) + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-
CC         (deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + beta-
CC         nicotinamide D-nucleotide.; EC=6.5.1.2; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_01587};
CC   -!- SIMILARITY: Belongs to the NAD-dependent DNA ligase family. LigB
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_01587}.
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DR   EMBL; CP000075; AAY39823.1; -; Genomic_DNA.
DR   RefSeq; WP_011269232.1; NC_007005.1.
DR   RefSeq; YP_237861.1; NC_007005.1.
DR   AlphaFoldDB; Q4ZLZ9; -.
DR   SMR; Q4ZLZ9; -.
DR   STRING; 205918.Psyr_4796; -.
DR   EnsemblBacteria; AAY39823; AAY39823; Psyr_4796.
DR   KEGG; psb:Psyr_4796; -.
DR   PATRIC; fig|205918.7.peg.4955; -.
DR   eggNOG; COG0272; Bacteria.
DR   HOGENOM; CLU_489786_0_0_6; -.
DR   OMA; DLWIQPK; -.
DR   Proteomes; UP000000426; Chromosome.
DR   GO; GO:0003911; F:DNA ligase (NAD+) activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   Gene3D; 2.40.50.140; -; 1.
DR   HAMAP; MF_01587; DNA_ligase_B; 1.
DR   InterPro; IPR020923; DNA_ligase_B.
DR   InterPro; IPR033136; DNA_ligase_CS.
DR   InterPro; IPR013839; DNAligase_adenylation.
DR   InterPro; IPR013840; DNAligase_N.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR004150; NAD_DNA_ligase_OB.
DR   InterPro; IPR010994; RuvA_2-like.
DR   Pfam; PF01653; DNA_ligase_aden; 1.
DR   Pfam; PF03120; DNA_ligase_OB; 1.
DR   SMART; SM00532; LIGANc; 1.
DR   SUPFAM; SSF47781; SSF47781; 1.
DR   SUPFAM; SSF50249; SSF50249; 1.
DR   PROSITE; PS01056; DNA_LIGASE_N2; 1.
PE   3: Inferred from homology;
KW   DNA damage; DNA repair; DNA replication; Ligase; NAD.
FT   CHAIN           1..561
FT                   /note="DNA ligase B"
FT                   /id="PRO_0000313549"
FT   ACT_SITE        128
FT                   /note="N6-AMP-lysine intermediate"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01587"
SQ   SEQUENCE   561 AA;  62111 MW;  B96AC199D716CEC4 CRC64;
     MLPTFRLMTC AIVFVLPGLG HASQCPDWPA AKARSEVTAL QQQIAEWDDS YHRQGISQIA
     DELYDQARQK LNLWRSCFAV STPESDPLKT AVGPLPHPVP HTGVNKLADE GSVKGWLKGR
     NDLWIQPKVD GVAVTLVYEK GRLVQAISRG DGRKGQDWTS QARLIKAIAQ QLPQAESTIL
     QGELYWRLDD HIQAASGSVN ARSKVAGLLA RQTISPQQAD AIGLFVWDWP NGPADMAQRL
     AGLQAMGFED SAAYTHPLEN YVQAARWREH WYRNPLPFAT DGVILRQGQR PPAQRWQASA
     PYWIAAWKHP YAQALAEVRK VNFKIGRSGR ITPVLELTPV RLDDRTVSRI STGSLQRWQT
     LDIRPGDQIA VSLAGLTIPR LDGVVSRAAE RADMIIPRAD DFHELSCWQA TPGCESQFRA
     RLAWLSGKKG LALPGVGPGT WNTLIDNGQI MGLLDWMTLN HAELVNIPGF AERSSAKLLD
     SLQTARERPF QTWLKAIGLP PTGGARLPDN WHELAGRSVE QWQAEPGIGP GRAARLRAFF
     QDPQVQALSQ QLQAQSISGF K