ID   LIGB_YERE8              Reviewed;         565 AA.
AC   A1JHV9;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   06-FEB-2007, sequence version 1.
DT   03-AUG-2022, entry version 75.
DE   RecName: Full=DNA ligase B {ECO:0000255|HAMAP-Rule:MF_01587};
DE            EC=6.5.1.2 {ECO:0000255|HAMAP-Rule:MF_01587};
DE   AltName: Full=Polydeoxyribonucleotide synthase [NAD(+)] B {ECO:0000255|HAMAP-Rule:MF_01587};
GN   Name=ligB {ECO:0000255|HAMAP-Rule:MF_01587}; OrderedLocusNames=YE0048;
OS   Yersinia enterocolitica serotype O:8 / biotype 1B (strain NCTC 13174 /
OS   8081).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Yersiniaceae; Yersinia.
OX   NCBI_TaxID=393305;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NCTC 13174 / 8081;
RX   PubMed=17173484; DOI=10.1371/journal.pgen.0020206;
RA   Thomson N.R., Howard S., Wren B.W., Holden M.T.G., Crossman L.,
RA   Challis G.L., Churcher C., Mungall K., Brooks K., Chillingworth T.,
RA   Feltwell T., Abdellah Z., Hauser H., Jagels K., Maddison M., Moule S.,
RA   Sanders M., Whitehead S., Quail M.A., Dougan G., Parkhill J.,
RA   Prentice M.B.;
RT   "The complete genome sequence and comparative genome analysis of the high
RT   pathogenicity Yersinia enterocolitica strain 8081.";
RL   PLoS Genet. 2:2039-2051(2006).
CC   -!- FUNCTION: Catalyzes the formation of phosphodiester linkages between
CC       5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD
CC       as a coenzyme and as the energy source for the reaction.
CC       {ECO:0000255|HAMAP-Rule:MF_01587}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=NAD(+) + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-
CC         (deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + beta-
CC         nicotinamide D-nucleotide.; EC=6.5.1.2; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_01587};
CC   -!- SIMILARITY: Belongs to the NAD-dependent DNA ligase family. LigB
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_01587}.
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DR   EMBL; AM286415; CAL10192.1; -; Genomic_DNA.
DR   RefSeq; WP_011815269.1; NC_008800.1.
DR   RefSeq; YP_001004444.1; NC_008800.1.
DR   AlphaFoldDB; A1JHV9; -.
DR   SMR; A1JHV9; -.
DR   STRING; 393305.YE0048; -.
DR   EnsemblBacteria; CAL10192; CAL10192; YE0048.
DR   KEGG; yen:YE0048; -.
DR   PATRIC; fig|393305.7.peg.138; -.
DR   eggNOG; COG0272; Bacteria.
DR   HOGENOM; CLU_489786_0_0_6; -.
DR   OMA; DLWIQPK; -.
DR   Proteomes; UP000000642; Chromosome.
DR   GO; GO:0003911; F:DNA ligase (NAD+) activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   Gene3D; 2.40.50.140; -; 1.
DR   HAMAP; MF_01587; DNA_ligase_B; 1.
DR   InterPro; IPR020923; DNA_ligase_B.
DR   InterPro; IPR013839; DNAligase_adenylation.
DR   InterPro; IPR013840; DNAligase_N.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR004150; NAD_DNA_ligase_OB.
DR   InterPro; IPR010994; RuvA_2-like.
DR   Pfam; PF01653; DNA_ligase_aden; 1.
DR   Pfam; PF03120; DNA_ligase_OB; 1.
DR   SMART; SM00532; LIGANc; 1.
DR   SUPFAM; SSF47781; SSF47781; 1.
DR   SUPFAM; SSF50249; SSF50249; 1.
PE   3: Inferred from homology;
KW   DNA damage; DNA repair; DNA replication; Ligase; NAD.
FT   CHAIN           1..565
FT                   /note="DNA ligase B"
FT                   /id="PRO_0000313557"
FT   ACT_SITE        130
FT                   /note="N6-AMP-lysine intermediate"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01587"
SQ   SEQUENCE   565 AA;  63940 MW;  8C4A6049F16C20C9 CRC64;
     MNVHKMKILS LLMVSFISWQ ARAESVCPEW SEERMSGEMH LLEKQLDQWN IAYHQQGISP
     IADDIYDQLQ DKLHRWRLCL GLPDKTDNRP IPGNGKMLHP VAHTGLKKLK DEAALISWMT
     GRKNLWVQPK IDGVAVTLVY QAGKLTQVLS RGNGLKGQNW ADKAPFISAI PQYIASAPPL
     LTLQGEVFLQ MEGHQQAQSG GANARASVAG ALMRKSVSPL LAKLGIFIWA WPDGPKSMVE
     KSRLLQEMGF PLTAHYSEPV ISSSDVALWR DRWFKMPLPF VTDGVVIRQE NVPAGRYWQA
     TPGNWSVAWK YPPPQQITEI KDIHFTVGRT GKITAILQVI PVKIDDKWIR RVNIGSIARW
     KQWDIVPGDQ VTISLAGQGI PRLDKVIWRV SQRQEIVPPD ADKFHQLTCF RRLPFECEPQ
     FLSRLAWLSG TNGLDMQSVG NGLWRELIHH GFINGLLDWL SLSVEQIAAV PGIGQGRAEK
     IYQQFQRARQ QPFSQWLQAL GFPQGIPLDT SWHSLRQRSI AEWRLMPGIG QVRAKQINHF
     LHHPEVQMMA DFLSQQGIAG FSPEE