LIGB_YERPE
ID LIGB_YERPE Reviewed; 567 AA.
AC Q7CLB2; Q74YB0;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 99.
DE RecName: Full=DNA ligase B {ECO:0000255|HAMAP-Rule:MF_01587};
DE EC=6.5.1.2 {ECO:0000255|HAMAP-Rule:MF_01587};
DE AltName: Full=Polydeoxyribonucleotide synthase [NAD(+)] B {ECO:0000255|HAMAP-Rule:MF_01587};
GN Name=ligB {ECO:0000255|HAMAP-Rule:MF_01587};
GN OrderedLocusNames=YPO0041, y0100, YP_0042;
OS Yersinia pestis.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Yersiniaceae; Yersinia.
OX NCBI_TaxID=632;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CO-92 / Biovar Orientalis;
RX PubMed=11586360; DOI=10.1038/35097083;
RA Parkhill J., Wren B.W., Thomson N.R., Titball R.W., Holden M.T.G.,
RA Prentice M.B., Sebaihia M., James K.D., Churcher C.M., Mungall K.L.,
RA Baker S., Basham D., Bentley S.D., Brooks K., Cerdeno-Tarraga A.-M.,
RA Chillingworth T., Cronin A., Davies R.M., Davis P., Dougan G., Feltwell T.,
RA Hamlin N., Holroyd S., Jagels K., Karlyshev A.V., Leather S., Moule S.,
RA Oyston P.C.F., Quail M.A., Rutherford K.M., Simmonds M., Skelton J.,
RA Stevens K., Whitehead S., Barrell B.G.;
RT "Genome sequence of Yersinia pestis, the causative agent of plague.";
RL Nature 413:523-527(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=KIM10+ / Biovar Mediaevalis;
RX PubMed=12142430; DOI=10.1128/jb.184.16.4601-4611.2002;
RA Deng W., Burland V., Plunkett G. III, Boutin A., Mayhew G.F., Liss P.,
RA Perna N.T., Rose D.J., Mau B., Zhou S., Schwartz D.C., Fetherston J.D.,
RA Lindler L.E., Brubaker R.R., Plano G.V., Straley S.C., McDonough K.A.,
RA Nilles M.L., Matson J.S., Blattner F.R., Perry R.D.;
RT "Genome sequence of Yersinia pestis KIM.";
RL J. Bacteriol. 184:4601-4611(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=91001 / Biovar Mediaevalis;
RX PubMed=15368893; DOI=10.1093/dnares/11.3.179;
RA Song Y., Tong Z., Wang J., Wang L., Guo Z., Han Y., Zhang J., Pei D.,
RA Zhou D., Qin H., Pang X., Han Y., Zhai J., Li M., Cui B., Qi Z., Jin L.,
RA Dai R., Chen F., Li S., Ye C., Du Z., Lin W., Wang J., Yu J., Yang H.,
RA Wang J., Huang P., Yang R.;
RT "Complete genome sequence of Yersinia pestis strain 91001, an isolate
RT avirulent to humans.";
RL DNA Res. 11:179-197(2004).
CC -!- FUNCTION: Catalyzes the formation of phosphodiester linkages between
CC 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD
CC as a coenzyme and as the energy source for the reaction.
CC {ECO:0000255|HAMAP-Rule:MF_01587}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=NAD(+) + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-
CC (deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + beta-
CC nicotinamide D-nucleotide.; EC=6.5.1.2; Evidence={ECO:0000255|HAMAP-
CC Rule:MF_01587};
CC -!- SIMILARITY: Belongs to the NAD-dependent DNA ligase family. LigB
CC subfamily. {ECO:0000255|HAMAP-Rule:MF_01587}.
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DR EMBL; AL590842; CAL18731.1; -; Genomic_DNA.
DR EMBL; AE009952; AAM83694.1; -; Genomic_DNA.
DR EMBL; AE017042; AAS60323.1; -; Genomic_DNA.
DR PIR; AB0006; AB0006.
DR RefSeq; WP_002215674.1; NZ_WUCM01000015.1.
DR RefSeq; YP_002345137.1; NC_003143.1.
DR AlphaFoldDB; Q7CLB2; -.
DR SMR; Q7CLB2; -.
DR IntAct; Q7CLB2; 2.
DR STRING; 214092.YPO0041; -.
DR PaxDb; Q7CLB2; -.
DR DNASU; 1145047; -.
DR EnsemblBacteria; AAM83694; AAM83694; y0100.
DR EnsemblBacteria; AAS60323; AAS60323; YP_0042.
DR GeneID; 57974549; -.
DR KEGG; ype:YPO0041; -.
DR KEGG; ypk:y0100; -.
DR KEGG; ypm:YP_0042; -.
DR PATRIC; fig|214092.21.peg.264; -.
DR eggNOG; COG0272; Bacteria.
DR HOGENOM; CLU_489786_0_0_6; -.
DR OMA; DLWIQPK; -.
DR Proteomes; UP000000815; Chromosome.
DR Proteomes; UP000001019; Chromosome.
DR Proteomes; UP000002490; Chromosome.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0003911; F:DNA ligase (NAD+) activity; IBA:GO_Central.
DR GO; GO:0006288; P:base-excision repair, DNA ligation; IBA:GO_Central.
DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR CDD; cd00114; LIGANc; 1.
DR Gene3D; 2.40.50.140; -; 1.
DR HAMAP; MF_01587; DNA_ligase_B; 1.
DR InterPro; IPR020923; DNA_ligase_B.
DR InterPro; IPR013839; DNAligase_adenylation.
DR InterPro; IPR013840; DNAligase_N.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR004150; NAD_DNA_ligase_OB.
DR InterPro; IPR010994; RuvA_2-like.
DR Pfam; PF01653; DNA_ligase_aden; 1.
DR Pfam; PF03120; DNA_ligase_OB; 1.
DR SMART; SM00532; LIGANc; 1.
DR SUPFAM; SSF47781; SSF47781; 1.
DR SUPFAM; SSF50249; SSF50249; 1.
PE 3: Inferred from homology;
KW DNA damage; DNA repair; DNA replication; Ligase; NAD; Reference proteome.
FT CHAIN 1..567
FT /note="DNA ligase B"
FT /id="PRO_0000313558"
FT ACT_SITE 132
FT /note="N6-AMP-lysine intermediate"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01587"
SQ SEQUENCE 567 AA; 63612 MW; A523450CBCEC9283 CRC64;
MNILNLKIIM FLLISNTIVV GGAWATSTCP DWPATRIAVE INALEQQLNK WSAAYHQQGH
SPVTDDIYDQ LQDKLRVWQS CRGLPDKTES QPIPGKGQFL HPVAHTGLKK LKDETALTRW
MAGRKNLWVQ PKVDGVAVTL VYHGGKLVQL LSRGNGVKGQ NWTEKAPFIS AIPQYIANAP
ALLTLQGELF LLMDGHQQAK SGGVNARSTV AGALMRKSPS PLLAQVGVFI WAWPDGPTTM
KEKVALLQVM GFPFTAKYSE PVMSHLDVVQ WRQFWFQAPL PFVTDGVVVR QEEEPAGRYW
QATPGQWSMA WKYPPLQHIA EVKDIHFTLG RTGKGTVVLE VLPIKIDDKW IRRVNIGSVT
RWKQWDIAPG DHITLALAGH GIPRLDNVVW RVHQRNTITA PNWDKFHQLS CFQRLPHGCE
PQFLSRLIWL SGPGGLDIGG IGGGFWQELI HHELINDLVG WLLLTPEQIA SIPGIGNARA
EKIYQQFQRA KQQPFSRWLL ALGFPQVVSV DAQWQVVLRR SLSEWATMAG IGQMRAKQIK
HFLDHPDVQA LADFLSTQKV VGFELTE
