ID   LIGB_YERPS              Reviewed;         567 AA.
AC   Q66GE4;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   11-OCT-2004, sequence version 1.
DT   03-AUG-2022, entry version 95.
DE   RecName: Full=DNA ligase B {ECO:0000255|HAMAP-Rule:MF_01587};
DE            EC=6.5.1.2 {ECO:0000255|HAMAP-Rule:MF_01587};
DE   AltName: Full=Polydeoxyribonucleotide synthase [NAD(+)] B {ECO:0000255|HAMAP-Rule:MF_01587};
GN   Name=ligB {ECO:0000255|HAMAP-Rule:MF_01587}; OrderedLocusNames=YPTB0038;
OS   Yersinia pseudotuberculosis serotype I (strain IP32953).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Yersiniaceae; Yersinia.
OX   NCBI_TaxID=273123;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=IP32953;
RX   PubMed=15358858; DOI=10.1073/pnas.0404012101;
RA   Chain P.S.G., Carniel E., Larimer F.W., Lamerdin J., Stoutland P.O.,
RA   Regala W.M., Georgescu A.M., Vergez L.M., Land M.L., Motin V.L.,
RA   Brubaker R.R., Fowler J., Hinnebusch J., Marceau M., Medigue C.,
RA   Simonet M., Chenal-Francisque V., Souza B., Dacheux D., Elliott J.M.,
RA   Derbise A., Hauser L.J., Garcia E.;
RT   "Insights into the evolution of Yersinia pestis through whole-genome
RT   comparison with Yersinia pseudotuberculosis.";
RL   Proc. Natl. Acad. Sci. U.S.A. 101:13826-13831(2004).
CC   -!- FUNCTION: Catalyzes the formation of phosphodiester linkages between
CC       5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD
CC       as a coenzyme and as the energy source for the reaction.
CC       {ECO:0000255|HAMAP-Rule:MF_01587}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=NAD(+) + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-
CC         (deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + beta-
CC         nicotinamide D-nucleotide.; EC=6.5.1.2; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_01587};
CC   -!- SIMILARITY: Belongs to the NAD-dependent DNA ligase family. LigB
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_01587}.
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DR   EMBL; BX936398; CAH19278.1; -; Genomic_DNA.
DR   RefSeq; WP_011191447.1; NZ_CP009712.1.
DR   AlphaFoldDB; Q66GE4; -.
DR   SMR; Q66GE4; -.
DR   EnsemblBacteria; CAH19278; CAH19278; YPTB0038.
DR   GeneID; 66843566; -.
DR   KEGG; ypo:BZ17_2557; -.
DR   KEGG; yps:YPTB0038; -.
DR   PATRIC; fig|273123.14.peg.2682; -.
DR   OMA; DLWIQPK; -.
DR   Proteomes; UP000001011; Chromosome.
DR   GO; GO:0003911; F:DNA ligase (NAD+) activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   CDD; cd00114; LIGANc; 1.
DR   Gene3D; 2.40.50.140; -; 1.
DR   HAMAP; MF_01587; DNA_ligase_B; 1.
DR   InterPro; IPR020923; DNA_ligase_B.
DR   InterPro; IPR013839; DNAligase_adenylation.
DR   InterPro; IPR013840; DNAligase_N.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR004150; NAD_DNA_ligase_OB.
DR   InterPro; IPR010994; RuvA_2-like.
DR   Pfam; PF01653; DNA_ligase_aden; 1.
DR   Pfam; PF03120; DNA_ligase_OB; 1.
DR   SMART; SM00532; LIGANc; 1.
DR   SUPFAM; SSF47781; SSF47781; 1.
DR   SUPFAM; SSF50249; SSF50249; 1.
PE   3: Inferred from homology;
KW   DNA damage; DNA repair; DNA replication; Ligase; NAD.
FT   CHAIN           1..567
FT                   /note="DNA ligase B"
FT                   /id="PRO_0000313562"
FT   ACT_SITE        132
FT                   /note="N6-AMP-lysine intermediate"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01587"
SQ   SEQUENCE   567 AA;  63623 MW;  EFE6A7D5BCE2951F CRC64;
     MNILNLKIIM FLLISNIIVV GGAWATSTCP DWPATRIAVE INALEQQLNK WSAAYHQQGH
     SPVTDDIYDQ LQDKLRVWQS CRGLPDKTES QPIPGKGQFL HPVAHTGLKK LKDETALTRW
     MAGRKNLWVQ PKVDGVAVTL VYHGGKLVQL LSRGNGVKGQ NWTEKAPFIS AIPQYIANAP
     ALLTLQGELF LLMDGHQQAK SGGVNARSTV AGALMRKSPS PLLAQVGVFI WAWPDGPTTM
     KEKVALLQVM GFPFTAKYSE PVMSHLDVVQ WRQFWFQAPL PFVTDGVVVR QEEEPAGRYW
     QATPGQWSMA WKYPPLQHIA EVKDIHFTLG RTGKGTVVLE VLPIKIDDKW IRRVNIGSVT
     RWKQWDIAPG DHITLALAGH GIPRLDNVVW RVHQRNTITA PNWDKFHQLS CFQRLPHGCE
     PQFLSRLIWL SGPGGLDIGG IGGGFWQKLI HHELINDLVG WLLLTPEQIA SIPGIGNARA
     EKIYQQFQRA KQQPFSRWLL ALGFPQVVSV DAQWQVVLRR SLSEWATMAG IGQMRAKQIK
     HFLDHPDVQA LADFLSTQKV VGFELTE