LIGC1_MYCS2
ID LIGC1_MYCS2 Reviewed; 348 AA.
AC A0R5T2;
DT 19-MAR-2014, integrated into UniProtKB/Swiss-Prot.
DT 09-JAN-2007, sequence version 1.
DT 03-AUG-2022, entry version 94.
DE RecName: Full=DNA ligase C1;
DE EC=6.5.1.1 {ECO:0000255|PROSITE-ProRule:PRU10135};
DE AltName: Full=Polydeoxyribonucleotide synthase [ATP];
GN Name=ligC; OrderedLocusNames=MSMEG_6302, MSMEI_6137;
OS Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium
OS smegmatis).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycolicibacterium.
OX NCBI_TaxID=246196;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RA Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C.,
RA Fraser C.M.;
RL Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=17295914; DOI=10.1186/gb-2007-8-2-r20;
RA Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C.,
RA Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.;
RT "Interrupted coding sequences in Mycobacterium smegmatis: authentic
RT mutations or sequencing errors?";
RL Genome Biol. 8:R20.1-R20.9(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=18955433; DOI=10.1101/gr.081901.108;
RA Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M.,
RA Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.;
RT "Ortho-proteogenomics: multiple proteomes investigation through orthology
RT and a new MS-based protocol.";
RL Genome Res. 19:128-135(2009).
RN [4]
RP FUNCTION, COFACTOR, AND SUBUNIT.
RX PubMed=14985346; DOI=10.1074/jbc.m401841200;
RA Gong C., Martins A., Bongiorno P., Glickman M., Shuman S.;
RT "Biochemical and genetic analysis of the four DNA ligases of
RT mycobacteria.";
RL J. Biol. Chem. 279:20594-20606(2004).
RN [5]
RP DISRUPTION PHENOTYPE.
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=15778718; DOI=10.1038/nsmb915;
RA Gong C., Bongiorno P., Martins A., Stephanou N.C., Zhu H., Shuman S.,
RA Glickman M.S.;
RT "Mechanism of nonhomologous end-joining in mycobacteria: a low-fidelity
RT repair system driven by Ku, ligase D and ligase C.";
RL Nat. Struct. Mol. Biol. 12:304-312(2005).
RN [6]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=18281464; DOI=10.1101/gad.1631908;
RA Aniukwu J., Glickman M.S., Shuman S.;
RT "The pathways and outcomes of mycobacterial NHEJ depend on the structure of
RT the broken DNA ends.";
RL Genes Dev. 22:512-527(2008).
CC -!- FUNCTION: DNA ligase that seals nicks in double-stranded DNA during DNA
CC replication, DNA recombination and DNA repair (By similarity). Has weak
CC intrinsic nick joining activities and accumulates DNA-adenylate. Acts
CC as a backup for LigD in the Ku-LigD-dependent NHEJ pathway.
CC {ECO:0000250, ECO:0000269|PubMed:14985346,
CC ECO:0000269|PubMed:18281464}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-
CC (deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP +
CC diphosphate.; EC=6.5.1.1; Evidence={ECO:0000255|PROSITE-
CC ProRule:PRU10135};
CC -!- COFACTOR:
CC Name=a divalent metal cation; Xref=ChEBI:CHEBI:60240;
CC Evidence={ECO:0000250};
CC -!- DISRUPTION PHENOTYPE: Not essential; a double ligC1-ligC2 mutant grows
CC normally, no effect on NHEJ. In a triple deletion with ligD NHEJ on
CC blunt-ended plasmid is 90-fold impaired. In quadruple ligB-ligC1-ligC2-
CC ligD deletions NHEJ on blunt and 5'-overhangs is 0.22 and 0.12% of
CC wild-type respectively, only 4-fold decrease in 3'-overhang NHEJ.
CC {ECO:0000269|PubMed:15778718, ECO:0000269|PubMed:18281464}.
CC -!- SIMILARITY: Belongs to the ATP-dependent DNA ligase family.
CC {ECO:0000305}.
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DR EMBL; CP000480; ABK76125.1; -; Genomic_DNA.
DR EMBL; CP001663; AFP42567.1; -; Genomic_DNA.
DR RefSeq; YP_890520.1; NC_008596.1.
DR AlphaFoldDB; A0R5T2; -.
DR SMR; A0R5T2; -.
DR STRING; 246196.MSMEI_6137; -.
DR EnsemblBacteria; ABK76125; ABK76125; MSMEG_6302.
DR EnsemblBacteria; AFP42567; AFP42567; MSMEI_6137.
DR KEGG; msg:MSMEI_6137; -.
DR KEGG; msm:MSMEG_6302; -.
DR PATRIC; fig|246196.19.peg.6138; -.
DR eggNOG; COG1793; Bacteria.
DR OMA; YRPHKSQ; -.
DR Proteomes; UP000000757; Chromosome.
DR Proteomes; UP000006158; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR GO; GO:0003910; F:DNA ligase (ATP) activity; IEA:UniProtKB-EC.
DR GO; GO:0006310; P:DNA recombination; IEA:InterPro.
DR GO; GO:0006281; P:DNA repair; IEA:InterPro.
DR CDD; cd07905; Adenylation_DNA_ligase_LigC; 1.
DR CDD; cd07970; OBF_DNA_ligase_LigC; 1.
DR Gene3D; 2.40.50.140; -; 1.
DR InterPro; IPR044119; Adenylation_LigC-like.
DR InterPro; IPR012309; DNA_ligase_ATP-dep_C.
DR InterPro; IPR012310; DNA_ligase_ATP-dep_cent.
DR InterPro; IPR016059; DNA_ligase_ATP-dep_CS.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR044117; OBF_LigC-like.
DR Pfam; PF04679; DNA_ligase_A_C; 1.
DR Pfam; PF01068; DNA_ligase_A_M; 1.
DR SUPFAM; SSF50249; SSF50249; 1.
DR PROSITE; PS00697; DNA_LIGASE_A1; 1.
PE 1: Evidence at protein level;
KW Ligase; Reference proteome.
FT CHAIN 1..348
FT /note="DNA ligase C1"
FT /id="PRO_0000425951"
FT ACT_SITE 32
FT /note="N6-AMP-lysine intermediate"
FT /evidence="ECO:0000305"
SQ SEQUENCE 348 AA; 39495 MW; 84664C237963598C CRC64;
MDLPVQPPIE PMLAKAQVKV PDEAGVWSYE PKWDGFRALV FRDGDDVVLQ SRNGKDLGRY
FPELLDALRD ELVEKCVLDG EVVVPRDIAG RVRLDWESLS QRIHPAASRI KMLAEQTPAH
FIGFDALALG DRSLLKEPFR VRREALAEAV DNKRWCHVTR TSEDPALGTE WLKTFEGAGL
DGVIAKRLDG PYLPGKREMV KVKHHRDADC VAMGYRIHKS GDGIGSILLG LYRDDGELQM
VGGAASFTAK DRIKLLAELE PLREGDEMRE GDPSRWNSAA DKRWTPLRPE KVCEVAYDQM
EGNSVEGRRF RHAVKFLRWR PDREPSSCTF DQLDTPLNYD LYDVLEEQ
