ID   LIGF_SPHSK              Reviewed;         257 AA.
AC   P30347;
DT   01-APR-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1993, sequence version 1.
DT   03-AUG-2022, entry version 73.
DE   RecName: Full=Protein LigF;
GN   Name=ligF;
OS   Sphingobium sp. (strain NBRC 103272 / SYK-6).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales;
OC   Sphingomonadaceae; Sphingobium.
OX   NCBI_TaxID=627192;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=NBRC 103272 / SYK-6;
RX   PubMed=1744051; DOI=10.1128/jb.173.24.7950-7955.1991;
RA   Masai E., Katayama Y., Kawai S., Nishikawa S., Yamasaki M., Morohoshi N.;
RT   "Cloning and sequencing of the gene for a Pseudomonas paucimobilis enzyme
RT   that cleaves beta-aryl ether.";
RL   J. Bacteriol. 173:7950-7955(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=NBRC 103272 / SYK-6;
RX   PubMed=8495726; DOI=10.1016/0014-5793(93)81465-c;
RA   Masai E., Katayama Y., Kubota S., Kawai S., Yamasaki M., Morohoshi N.;
RT   "A bacterial enzyme degrading the model lignin compound beta-etherase is a
RT   member of the glutathione-S-transferase superfamily.";
RL   FEBS Lett. 323:135-140(1993).
CC   -!- FUNCTION: Lignin degradation enzyme.
CC   -!- SIMILARITY: Belongs to the GST superfamily. {ECO:0000305}.
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DR   EMBL; D11473; BAA02031.1; -; Genomic_DNA.
DR   PIR; S33314; S33314.
DR   RefSeq; WP_014075191.1; NC_015976.1.
DR   PDB; 4XT0; X-ray; 2.07 A; A=1-243.
DR   PDBsum; 4XT0; -.
DR   AlphaFoldDB; P30347; -.
DR   SMR; P30347; -.
DR   STRING; 627192.SLG_08650; -.
DR   BioCyc; MetaCyc:MON-15129; -.
DR   GO; GO:0006749; P:glutathione metabolic process; IEA:InterPro.
DR   GO; GO:0046274; P:lignin catabolic process; IEA:UniProtKB-KW.
DR   InterPro; IPR010987; Glutathione-S-Trfase_C-like.
DR   InterPro; IPR036282; Glutathione-S-Trfase_C_sf.
DR   InterPro; IPR040079; Glutathione_S-Trfase.
DR   InterPro; IPR004045; Glutathione_S-Trfase_N.
DR   InterPro; IPR004046; GST_C.
DR   InterPro; IPR036249; Thioredoxin-like_sf.
DR   Pfam; PF00043; GST_C; 1.
DR   Pfam; PF02798; GST_N; 1.
DR   SFLD; SFLDS00019; Glutathione_Transferase_(cytos; 1.
DR   SUPFAM; SSF47616; SSF47616; 1.
DR   SUPFAM; SSF52833; SSF52833; 1.
DR   PROSITE; PS50405; GST_CTER; 1.
DR   PROSITE; PS50404; GST_NTER; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Lignin degradation.
FT   CHAIN           1..257
FT                   /note="Protein LigF"
FT                   /id="PRO_0000186042"
FT   DOMAIN          1..82
FT                   /note="GST N-terminal"
FT   DOMAIN          89..257
FT                   /note="GST C-terminal"
FT   STRAND          3..11
FT                   /evidence="ECO:0007829|PDB:4XT0"
FT   HELIX           12..23
FT                   /evidence="ECO:0007829|PDB:4XT0"
FT   STRAND          29..32
FT                   /evidence="ECO:0007829|PDB:4XT0"
FT   HELIX           35..37
FT                   /evidence="ECO:0007829|PDB:4XT0"
FT   HELIX           39..41
FT                   /evidence="ECO:0007829|PDB:4XT0"
FT   HELIX           43..48
FT                   /evidence="ECO:0007829|PDB:4XT0"
FT   STRAND          56..59
FT                   /evidence="ECO:0007829|PDB:4XT0"
FT   STRAND          62..66
FT                   /evidence="ECO:0007829|PDB:4XT0"
FT   HELIX           67..77
FT                   /evidence="ECO:0007829|PDB:4XT0"
FT   STRAND          81..83
FT                   /evidence="ECO:0007829|PDB:4XT0"
FT   HELIX           90..105
FT                   /evidence="ECO:0007829|PDB:4XT0"
FT   HELIX           107..118
FT                   /evidence="ECO:0007829|PDB:4XT0"
FT   HELIX           121..125
FT                   /evidence="ECO:0007829|PDB:4XT0"
FT   HELIX           129..139
FT                   /evidence="ECO:0007829|PDB:4XT0"
FT   HELIX           143..154
FT                   /evidence="ECO:0007829|PDB:4XT0"
FT   HELIX           158..179
FT                   /evidence="ECO:0007829|PDB:4XT0"
FT   STRAND          182..186
FT                   /evidence="ECO:0007829|PDB:4XT0"
FT   HELIX           192..201
FT                   /evidence="ECO:0007829|PDB:4XT0"
FT   TURN            202..205
FT                   /evidence="ECO:0007829|PDB:4XT0"
FT   TURN            210..214
FT                   /evidence="ECO:0007829|PDB:4XT0"
FT   TURN            217..219
FT                   /evidence="ECO:0007829|PDB:4XT0"
FT   HELIX           221..232
FT                   /evidence="ECO:0007829|PDB:4XT0"
FT   HELIX           234..241
FT                   /evidence="ECO:0007829|PDB:4XT0"
SQ   SEQUENCE   257 AA;  29686 MW;  900E30C3BB3AE08B CRC64;
     MTLKLYSFGP GANSLKPLAT LYEKGLEFEQ VFVDPSKFEQ HSDWFKKINP RGQVPALWHD
     GKVVTESTVI CEYLEDVFPE SGNSLRPADP FKRAEMRVWT KWVDEYFCWC VSTIGWAFGI
     KAIAQKMSDE EFEEHINKNV PIPEQQLKWR RARNGFPQEM LDEEFRKVGV SVARLEETLS
     KQDYLVDTGY SLADICNFAI ANGLQRPGGF FGDYVNQEKT PGLCAWLDRI NARPAIKEMF
     EKSKREDLLK RQNEKVA