LIG_DROME
ID LIG_DROME Reviewed; 1375 AA.
AC Q86S05; A1Z794; Q86S06; Q8SZZ7;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 03-AUG-2022, entry version 134.
DE RecName: Full=Protein lingerer;
GN Name=lig {ECO:0000312|EMBL:AAO23023.1, ECO:0000312|FlyBase:FBgn0020279};
GN ORFNames=CG8715;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1] {ECO:0000305, ECO:0000312|EMBL:AAO23023.1}
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS B AND D), FUNCTION, SUBCELLULAR
RP LOCATION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE, AND DEVELOPMENTAL
RP STAGE.
RX PubMed=12524348; DOI=10.1093/genetics/162.4.1775;
RA Kuniyoshi H., Baba K., Ueda R., Kondo S., Awano W., Juni N., Yamamoto D.;
RT "Lingerer, a Drosophila gene involved in initiation and termination of
RT copulation, encodes a set of novel cytoplasmic proteins.";
RL Genetics 162:1775-1789(2002).
RN [2] {ECO:0000312|EMBL:AAX52724.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000269|PubMed:10731132};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3] {ECO:0000305, ECO:0000312|EMBL:AAX52724.1}
RP GENOME REANNOTATION, AND ALTERNATIVE SPLICING.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4] {ECO:0000305, ECO:0000312|EMBL:AAL39827.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM B).
RC STRAIN=Berkeley {ECO:0000312|EMBL:AAL39827.1};
RC TISSUE=Embryo {ECO:0000269|PubMed:12537569};
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [5]
RP TISSUE SPECIFICITY.
RX PubMed=14668197; DOI=10.1080/neg.17.2-3.117.137;
RA Kuniyoshi H., Usui-Aoki K., Juni N., Yamamoto D.;
RT "Expression analysis of the lingerer gene in the larval central nervous
RT system of Drosophila melanogaster.";
RL J. Neurogenet. 17:117-137(2003).
RN [6] {ECO:0000305}
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-672; THR-673 AND SER-674, AND
RP IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=17372656; DOI=10.1039/b617545g;
RA Bodenmiller B., Mueller L.N., Pedrioli P.G.A., Pflieger D., Juenger M.A.,
RA Eng J.K., Aebersold R., Tao W.A.;
RT "An integrated chemical, mass spectrometric and computational strategy for
RT (quantitative) phosphoproteomics: application to Drosophila melanogaster
RT Kc167 cells.";
RL Mol. Biosyst. 3:275-286(2007).
RN [7]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-321 AND SER-324, AND
RP IDENTIFICATION BY MASS SPECTROMETRY.
RC TISSUE=Embryo;
RX PubMed=18327897; DOI=10.1021/pr700696a;
RA Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
RT "Phosphoproteome analysis of Drosophila melanogaster embryos.";
RL J. Proteome Res. 7:1675-1682(2008).
CC -!- FUNCTION: Acts in the nervous system to mediate the control of
CC copulatory organs during courtship. {ECO:0000269|PubMed:12524348}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:12524348}.
CC Note=Found in the cytoplasm of many neuronal and glial cells in the
CC brain and ventral ganglion, and in imaginal disks.
CC {ECO:0000269|PubMed:12524348}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=D {ECO:0000269|PubMed:12524348}; Synonyms=Lig-B
CC {ECO:0000269|PubMed:12524348}, type2 {ECO:0000269|PubMed:12524348};
CC IsoId=Q86S05-1; Sequence=Displayed;
CC Name=A {ECO:0000269|PubMed:10731132}; Synonyms=C
CC {ECO:0000269|PubMed:10731132};
CC IsoId=Q86S05-2; Sequence=VSP_052633;
CC Name=B {ECO:0000269|PubMed:12524348}; Synonyms=Lig-A
CC {ECO:0000269|PubMed:12524348}, type1 {ECO:0000269|PubMed:12524348},
CC type3 {ECO:0000269|PubMed:12524348};
CC IsoId=Q86S05-3; Sequence=VSP_052634;
CC -!- TISSUE SPECIFICITY: At stage 11, expression is restricted to the
CC neuroblasts, predominant in the central nervous system (CNS), including
CC the brain and ventral nerve cord, and in the PNS. Later embryonic
CC expression is seen in the gonads. Late third instar larvae show
CC expression in the CNS, imaginal disks (including genital, eye-antennal,
CC leg, wing and haltere disks), and gonads. In the larval brain, it is
CC expressed in all of the glial cells and in clusters of neurons that
CC projected contralaterally. In the larval ventral ganglion, it is
CC expressed in subperineurial glia, peripheral exit glia, and a number of
CC interneurons, but not in motor neurons. Isoform B is abundantly
CC expressed in males and females. Isoform D is male specific and
CC expressed at low levels. {ECO:0000269|PubMed:12524348,
CC ECO:0000269|PubMed:14668197}.
CC -!- DEVELOPMENTAL STAGE: Expressed both maternally and zygotically
CC throughout development. Zygotic expression levels rise after embryonic
CC stage 9. {ECO:0000269|PubMed:12524348}.
CC -!- DISRUPTION PHENOTYPE: Male flies exhibit abnormal copulation. They fail
CC to successfully withdraw their genitalia upon termination of copulation
CC so end up 'stuck' to the female, tugging to separate. There is also a
CC 'non-copulating' behavioral phenotype. There are no morphological
CC defects in the male genitalia. Females can mate successfully but have
CC reduced fertility. Complete loss of lig function results in lethality
CC during early pupal stages. {ECO:0000269|PubMed:12524348}.
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DR EMBL; AF276963; AAO23022.1; -; mRNA.
DR EMBL; AF276964; AAO23023.1; -; mRNA.
DR EMBL; AE013599; AAF59144.3; -; Genomic_DNA.
DR EMBL; AE013599; AAM68848.2; -; Genomic_DNA.
DR EMBL; AE013599; AAS64895.2; -; Genomic_DNA.
DR EMBL; AE013599; AAX52724.1; -; Genomic_DNA.
DR EMBL; AY069682; AAL39827.1; -; mRNA.
DR RefSeq; NP_001014503.1; NM_001014503.2. [Q86S05-1]
DR RefSeq; NP_001163081.1; NM_001169610.2. [Q86S05-3]
DR RefSeq; NP_001163082.1; NM_001169611.1. [Q86S05-3]
DR RefSeq; NP_001163083.1; NM_001169612.2. [Q86S05-3]
DR RefSeq; NP_610348.3; NM_136504.5. [Q86S05-2]
DR RefSeq; NP_724643.2; NM_165586.5. [Q86S05-3]
DR RefSeq; NP_995778.2; NM_206056.5. [Q86S05-2]
DR AlphaFoldDB; Q86S05; -.
DR SMR; Q86S05; -.
DR BioGRID; 61631; 22.
DR IntAct; Q86S05; 2.
DR MINT; Q86S05; -.
DR STRING; 7227.FBpp0100030; -.
DR iPTMnet; Q86S05; -.
DR PaxDb; Q86S05; -.
DR DNASU; 35771; -.
DR EnsemblMetazoa; FBtr0088774; FBpp0087852; FBgn0020279. [Q86S05-2]
DR EnsemblMetazoa; FBtr0088775; FBpp0087853; FBgn0020279. [Q86S05-3]
DR EnsemblMetazoa; FBtr0088776; FBpp0087854; FBgn0020279. [Q86S05-2]
DR EnsemblMetazoa; FBtr0100575; FBpp0100030; FBgn0020279. [Q86S05-1]
DR EnsemblMetazoa; FBtr0301915; FBpp0291129; FBgn0020279. [Q86S05-3]
DR EnsemblMetazoa; FBtr0301916; FBpp0291130; FBgn0020279. [Q86S05-3]
DR EnsemblMetazoa; FBtr0301917; FBpp0291131; FBgn0020279. [Q86S05-3]
DR GeneID; 35771; -.
DR KEGG; dme:Dmel_CG8715; -.
DR CTD; 35771; -.
DR FlyBase; FBgn0020279; lig.
DR VEuPathDB; VectorBase:FBgn0020279; -.
DR eggNOG; ENOG502QPRH; Eukaryota.
DR GeneTree; ENSGT00390000003453; -.
DR InParanoid; Q86S05; -.
DR PhylomeDB; Q86S05; -.
DR SignaLink; Q86S05; -.
DR BioGRID-ORCS; 35771; 0 hits in 3 CRISPR screens.
DR ChiTaRS; lig; fly.
DR GenomeRNAi; 35771; -.
DR PRO; PR:Q86S05; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0020279; Expressed in wing disc and 22 other tissues.
DR ExpressionAtlas; Q86S05; baseline and differential.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0000932; C:P-body; IDA:FlyBase.
DR GO; GO:0007620; P:copulation; IMP:FlyBase.
DR GO; GO:0008069; P:dorsal/ventral axis specification, ovarian follicular epithelium; IGI:FlyBase.
DR GO; GO:0060179; P:male mating behavior; IMP:FlyBase.
DR GO; GO:0045571; P:negative regulation of imaginal disc growth; IGI:FlyBase.
DR GO; GO:0046426; P:negative regulation of receptor signaling pathway via JAK-STAT; IMP:FlyBase.
DR GO; GO:0035332; P:positive regulation of hippo signaling; IMP:FlyBase.
DR GO; GO:0010468; P:regulation of gene expression; IMP:FlyBase.
DR InterPro; IPR009060; UBA-like_sf.
DR InterPro; IPR022166; UBAP2/Lig.
DR Pfam; PF12478; DUF3697; 1.
DR SUPFAM; SSF46934; SSF46934; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Behavior; Cytoplasm; Phosphoprotein;
KW Reference proteome.
FT CHAIN 1..1375
FT /note="Protein lingerer"
FT /id="PRO_0000314621"
FT DOMAIN 84..124
FT /note="UBA"
FT /evidence="ECO:0000255"
FT REGION 1..69
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 142..286
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 374..453
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 613..646
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 750..801
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 869..894
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 987..1036
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1203..1234
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1251..1277
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 18..32
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 50..64
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 158..173
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 184..203
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 376..431
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 439..453
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 624..646
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1251..1276
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 321
FT /note="Phosphotyrosine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 324
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 672
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:17372656"
FT MOD_RES 673
FT /note="Phosphothreonine"
FT /evidence="ECO:0000269|PubMed:17372656"
FT MOD_RES 674
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:17372656"
FT VAR_SEQ 1301..1375
FT /note="VHSELPLQVVVGGGTESHVLIHGNVNGPQVTYHRIWQTEPVQCQNQLVAAHC
FT NNFEPNIDCCVHDNNNDLSFFAD -> MDGRIHSSSRRDSNSAGQRQQSTSQSKSAGKQ
FT GYSPSYWAGQN (in isoform A)"
FT /evidence="ECO:0000303|PubMed:10731132"
FT /id="VSP_052633"
FT VAR_SEQ 1301..1375
FT /note="VHSELPLQVVVGGGTESHVLIHGNVNGPQVTYHRIWQTEPVQCQNQLVAAHC
FT NNFEPNIDCCVHDNNNDLSFFAD -> DSNSAGQRQQSTSQSKSAGKQGYSPSYWAGQN
FT (in isoform B)"
FT /evidence="ECO:0000303|PubMed:12524348,
FT ECO:0000303|PubMed:12537569"
FT /id="VSP_052634"
FT CONFLICT 185
FT /note="R -> C (in Ref. 1; AAO23022)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1375 AA; 139491 MW; 5FE3204D18FDE70A CRC64;
MSTQTRSGGG GGGGHARNQK KSNASNSGGG GTGHHDGVSH AAAAGKKGGQ DASKTDKPEK
AQPKATTEQL RIAQITNSTT EDPQINEKVL LLLTMTQRSE EEVCCALNEC DYDLEAAANF
LIEELPQGAF AKYEKKRKNK AANNTADGAA GDGDWADGNG NADRREKSRN RSSNRGGTRG
SSDSRGWRGR ETRENERNQR ESREPWSGQN AGQDRGDDRA NDNYRGQRNG GGRSGPGGGG
RGGGFVSRSG RGGGRMGGRT GGPRGDRGSG GPGGAYGSGR GGNANEDHHE VELWDNTIAQ
NAEKQQQAHD DAWGDWNNEE YEGSLKDSKV FTTSNLATQS AANVVSGTGA SVTAVPAAAG
TEISAPPGLE HQLVQQGSHL EESSSSGPAA VTPPATLSGS ATTPLLQYSA AVSNPPPQLQ
SQGTQSGAGT GASAAAGGGA GSTPSSFVSA SPDTFSSAAS AAATLVHQAQ KQQQLQQQTT
PIKPSATLSV EQSQYFNSLA SQGVSPGSVP VQSAPAGYAQ NPVAAYSQTS TSVGVSQYPN
TYANVFASGT AAGAGTAEQS QQQPQIRRAR VKLPPPSKIP ASAVEMPGDN ALNNIGYLDV
QFGALDFGTD DGFEPLPEKV GSGFSIDGQQ QQQQPDDYQS KSQQQQQVTL AAGLQSSQIS
DALNAAGYTS RSTSQQQQGV SSAVNATIDQ LTKSDPYGQT GGSGNAYQNA YQSSGASKTA
SGFPTTAPGG YSSSTYANVQ SSVANSYQQQ GYGSYQPSSY QQQAGSGAQS GTGAVSGGGG
TATQNIPVGG SSSQNSTSGN ASSAYLTSGY STPQSAYQSS QSVYGNTGLS NSSGFSGSAS
NASSQYANFS ASAKLKDATT ASSAAHYDSV STSSGVSSNS GSTGNGGVVS GQTGANQAAV
SNNNSVSGSS SVSNVTAGVA SGNVAGVGGG VSQSGVSSGV GVPGGSASSV GVNVNNNSSS
ASSVGAATVA QTATGTTAAV LASLTNKNTS SSNSSGSGGS AATTTGNASG QGAGASTGGV
GSSSGAGGAG SGGGSGSGLV PTNIQMVSQY IQTGLPYYQQ PVYSYEELQM MQQRVPHVQG
YYDLNYPPAS LGAGRDNLGS VTYSAMNDGR FARTDNNSSP VGNVSSTMSQ QAGSSAPMLN
VPYAYFYGGN VMPGSFQYGT PAIYPQIPAA NTASGQQFPK PSYSAGYGST SYDTLSQTTQ
DYSKGGYSSS VNQQSKTQTV SNQSQAGTGS DLTSSMYGKG HVALNKVNSY EKQSFHSGTP
PPFNMPNTQT AGGTSAQPYG MYLPMPAAGH HNMIHQPIHQ VHSELPLQVV VGGGTESHVL
IHGNVNGPQV TYHRIWQTEP VQCQNQLVAA HCNNFEPNID CCVHDNNNDL SFFAD
