LILI_DROME
ID LILI_DROME Reviewed; 527 AA.
AC Q9VC35; Q86PC4; Q9XZ04;
DT 06-JUL-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 145.
DE RecName: Full=Protein Lilipod {ECO:0000303|PubMed:26512105};
GN Name=lili {ECO:0000303|PubMed:26512105};
GN ORFNames=CG5807 {ECO:0000312|FlyBase:FBgn0027539};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000312|Proteomes:UP000000803};
RN [1] {ECO:0000312|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2] {ECO:0000312|Proteomes:UP000000803}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3] {ECO:0000312|EMBL:AAD34745.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley {ECO:0000312|EMBL:AAD34745.1};
RC TISSUE=Head {ECO:0000312|EMBL:AAD34745.1};
RX PubMed=10731138; DOI=10.1126/science.287.5461.2222;
RA Rubin G.M., Hong L., Brokstein P., Evans-Holm M., Frise E., Stapleton M.,
RA Harvey D.A.;
RT "A Drosophila complementary DNA resource.";
RL Science 287:2222-2224(2000).
RN [4] {ECO:0000312|EMBL:AAO24967.1, ECO:0000312|EMBL:ACM16699.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley {ECO:0000312|EMBL:AAO24967.1};
RC TISSUE=Embryo {ECO:0000312|EMBL:AAO24967.1};
RA Booth B., Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J.,
RA Champe M., Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E.,
RA George R., Gonzalez M., Guarin H., Kronmiller B., Li P., Liao G.,
RA Miranda A., Mungall C.J., Nunoo J., Pacleb J., Paragas V., Park S.,
RA Patel S., Phouanenavong S., Wan K., Yu C., Lewis S.E., Rubin G.M.,
RA Celniker S.;
RL Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases.
RN [5] {ECO:0000305}
RP FUNCTION, TISSUE SPECIFICITY, AND DISRUPTION PHENOTYPE.
RX PubMed=26512105; DOI=10.1073/pnas.1509856112;
RA Dolezal D., Liu Z., Zhou Q., Pignoni F.;
RT "Fly LMBR1/LIMR-type protein Lilipod promotes germ-line stem cell self-
RT renewal by enhancing BMP signaling.";
RL Proc. Natl. Acad. Sci. U.S.A. 112:13928-13933(2015).
CC -!- FUNCTION: Required during oogenesis to promote self-renewal of germline
CC stem cells, probably by enhancing BMP signaling activity.
CC {ECO:0000269|PubMed:26512105}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305|PubMed:26512105};
CC Multi-pass membrane protein {ECO:0000255}.
CC -!- TISSUE SPECIFICITY: In the ovary, detected in germline stem cells and
CC their progeny. Also detected in the somatic follicular epithelium.
CC {ECO:0000269|PubMed:26512105}.
CC -!- DISRUPTION PHENOTYPE: Larval lethal. Conditional knockdown in the ovary
CC results in progressive loss of undifferentiated germline stem cells.
CC {ECO:0000269|PubMed:26512105}.
CC -!- SIMILARITY: Belongs to the LIMR family. {ECO:0000305}.
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DR EMBL; AE014297; AAF56340.1; -; Genomic_DNA.
DR EMBL; AF132157; AAD34745.1; -; mRNA.
DR EMBL; BT003212; AAO24967.1; -; mRNA.
DR EMBL; BT057989; ACM16699.1; -; mRNA.
DR RefSeq; NP_651290.1; NM_143033.3.
DR AlphaFoldDB; Q9VC35; -.
DR IntAct; Q9VC35; 2.
DR STRING; 7227.FBpp0084114; -.
DR PaxDb; Q9VC35; -.
DR PRIDE; Q9VC35; -.
DR DNASU; 42956; -.
DR EnsemblMetazoa; FBtr0084739; FBpp0084114; FBgn0027539.
DR GeneID; 42956; -.
DR KEGG; dme:Dmel_CG5807; -.
DR UCSC; CG5807-RA; d. melanogaster.
DR CTD; 42956; -.
DR FlyBase; FBgn0027539; lili.
DR VEuPathDB; VectorBase:FBgn0027539; -.
DR eggNOG; KOG3722; Eukaryota.
DR GeneTree; ENSGT00390000007809; -.
DR InParanoid; Q9VC35; -.
DR OrthoDB; 768229at2759; -.
DR PhylomeDB; Q9VC35; -.
DR BioGRID-ORCS; 42956; 0 hits in 3 CRISPR screens.
DR GenomeRNAi; 42956; -.
DR PRO; PR:Q9VC35; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0027539; Expressed in cleaving embryo and 26 other tissues.
DR ExpressionAtlas; Q9VC35; baseline and differential.
DR GO; GO:0005887; C:integral component of plasma membrane; ISS:FlyBase.
DR GO; GO:0004888; F:transmembrane signaling receptor activity; IBA:GO_Central.
DR GO; GO:0036099; P:female germ-line stem cell population maintenance; IMP:FlyBase.
DR GO; GO:0048477; P:oogenesis; IEA:UniProtKB-KW.
DR GO; GO:0030513; P:positive regulation of BMP signaling pathway; IGI:FlyBase.
DR GO; GO:0007165; P:signal transduction; IBA:GO_Central.
DR InterPro; IPR008075; LIMR.
DR InterPro; IPR006876; LMBR1-like_membr_prot.
DR PANTHER; PTHR12625; PTHR12625; 1.
DR Pfam; PF04791; LMBR1; 1.
DR PRINTS; PR01692; LIPOCALINIMR.
PE 2: Evidence at transcript level;
KW Cell membrane; Differentiation; Membrane; Oogenesis; Reference proteome;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..527
FT /note="Protein Lilipod"
FT /id="PRO_0000436844"
FT TOPO_DOM 1..22
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 23..43
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 44..65
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 66..86
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 87..117
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 118..138
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 139..160
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 161..181
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 182..194
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 195..215
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 216..341
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 342..362
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 363..395
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 396..416
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 417..433
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 434..454
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 455..468
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 469..489
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 490..527
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT CONFLICT 199
FT /note="F -> S (in Ref. 4; AAO24967)"
FT /evidence="ECO:0000305"
FT CONFLICT 403
FT /note="C -> R (in Ref. 3; AAD34745)"
FT /evidence="ECO:0000305"
FT CONFLICT 468
FT /note="D -> E (in Ref. 3; AAD34745)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 527 AA; 59993 MW; B8610F990B61F5A7 CRC64;
MDEEEEEEVT DLKLQLFHNT VREHIIFLLL IILLYSSSYV VVSRFRRRDR DDLYSNDEDE
VLVYRISFWL CTFTLAVAEG AAMLLPVSIA SNEVLLLYPN SYYVKWLNSS LIQGLWNHVF
LFSNLSLFIF LPFVYLFSES TGFVGNKKGI LPRVYETFTV FMLMAIIVLV LTAVLSAVFG
IEKLQFFWFL NLGSVHLPFL YSCVSFLGVM LMLICTPYGF VRLFGVVNQV LVRPMLLRDV
NEEFSAFYME EASVKRKLAH IELHNVSIAD AANGGRLGNR IGLGRGRTFY PQPLLQHSQA
HLYQRKAMAS DVDELNDRLR ELDSERKELD KLRTSSTFQR TFVYPLAMLL LLFCTAVTIL
LVVQNTLELL IGIKALPLST RQFALGISSL SKLGPFGAGL EVCLIFYLGA TSVVGFYSMP
FMRKVCPKRR QTSLPQLMLN CGFMLVLSSA LPLLSRIIGI TNFDLLGDFG AIEWLGNFQI
VLLYNLVFGT TTALCLANKF TATVRRELRA RLVENYVLFT NYISFIN
