LIM7_CAEEL
ID LIM7_CAEEL Reviewed; 452 AA.
AC G5EC36;
DT 07-OCT-2020, integrated into UniProtKB/Swiss-Prot.
DT 14-DEC-2011, sequence version 1.
DT 03-AUG-2022, entry version 91.
DE RecName: Full=LIM/homeobox protein lim-7 {ECO:0000305};
GN Name=lim-7 {ECO:0000312|WormBase:C04F1.3};
GN ORFNames=C04F1.3 {ECO:0000312|WormBase:C04F1.3};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN [1] {ECO:0000312|EMBL:AAB18328.1}
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=Bristol N2 {ECO:0000312|EMBL:AAB18328.1};
RA Hobert O., Ruvkun G.;
RT "CeLIM-7, a novel LIM homeobox gene from C. elegans.";
RL Submitted (OCT-1996) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000312|Proteomes:UP000001940}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [3] {ECO:0000305}
RP TISSUE SPECIFICITY, AND MUTAGENESIS OF 84-CYS--GLN-161.
RX PubMed=19116151; DOI=10.1016/j.febslet.2008.12.046;
RA Voutev R., Keating R., Hubbard E.J., Vallier L.G.;
RT "Characterization of the Caenorhabditis elegans Islet LIM-homeodomain
RT ortholog, lim-7.";
RL FEBS Lett. 583:456-464(2009).
RN [4] {ECO:0000305}
RP INTERACTION WITH CEH-14, AND TISSUE SPECIFICITY.
RX PubMed=28676648; DOI=10.1038/s41598-017-04587-8;
RA Bhati M., Llamosas E., Jacques D.A., Jeffries C.M., Dastmalchi S.,
RA Ripin N., Nicholas H.R., Matthews J.M.;
RT "Interactions between LHX3- and ISL1-family LIM-homeodomain transcription
RT factors are conserved in Caenorhabditis elegans.";
RL Sci. Rep. 7:4579-4579(2017).
CC -!- FUNCTION: Probable DNA-binding transcriptional activator.
CC {ECO:0000250|UniProtKB:P61372}.
CC -!- SUBUNIT: Interacts (via LID domain) with ceh-14 (via LIM zinc-binding
CC domains 1 and 2). {ECO:0000269|PubMed:28676648}.
CC -!- INTERACTION:
CC G5EC36; P20271: ceh-14; NbExp=3; IntAct=EBI-324631, EBI-2411837;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00108,
CC ECO:0000255|RuleBase:RU000682}.
CC -!- TISSUE SPECIFICITY: Expressed in gonadal sheath cells, URA motoneurons,
CC and 10 additional cells near the isthmus and terminal bulb of the
CC pharynx (PubMed:19116151). Expressed in the ALA and BDU cells
CC (PubMed:19116151). {ECO:0000269|PubMed:19116151}.
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DR EMBL; U73946; AAB18328.1; -; mRNA.
DR EMBL; BX284601; CCD62964.1; -; Genomic_DNA.
DR PIR; T33049; T33049.
DR RefSeq; NP_491668.1; NM_059267.1.
DR AlphaFoldDB; G5EC36; -.
DR SASBDB; G5EC36; -.
DR SMR; G5EC36; -.
DR IntAct; G5EC36; 20.
DR STRING; 6239.C04F1.3; -.
DR EPD; G5EC36; -.
DR PaxDb; G5EC36; -.
DR EnsemblMetazoa; C04F1.3.1; C04F1.3.1; WBGene00002989.
DR GeneID; 172236; -.
DR KEGG; cel:CELE_C04F1.3; -.
DR CTD; 172236; -.
DR WormBase; C04F1.3; CE17366; WBGene00002989; lim-7.
DR eggNOG; KOG0490; Eukaryota.
DR GeneTree; ENSGT00940000153731; -.
DR HOGENOM; CLU_027802_2_2_1; -.
DR InParanoid; G5EC36; -.
DR OMA; WDEERST; -.
DR OrthoDB; 948890at2759; -.
DR PhylomeDB; G5EC36; -.
DR Proteomes; UP000001940; Chromosome I.
DR Bgee; WBGene00002989; Expressed in pharyngeal muscle cell (C elegans) and 3 other tissues.
DR GO; GO:0005634; C:nucleus; IDA:WormBase.
DR GO; GO:0000987; F:cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0007409; P:axonogenesis; IBA:GO_Central.
DR GO; GO:0010171; P:body morphogenesis; IMP:WormBase.
DR GO; GO:0035262; P:gonad morphogenesis; IMP:WormBase.
DR GO; GO:0060323; P:head morphogenesis; IMP:WormBase.
DR GO; GO:0040011; P:locomotion; IMP:WormBase.
DR GO; GO:0002119; P:nematode larval development; IMP:WormBase.
DR GO; GO:0048665; P:neuron fate specification; IBA:GO_Central.
DR GO; GO:0060465; P:pharynx development; IMP:WormBase.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR001781; Znf_LIM.
DR Pfam; PF00046; Homeodomain; 1.
DR Pfam; PF00412; LIM; 2.
DR SMART; SM00389; HOX; 1.
DR SMART; SM00132; LIM; 2.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
DR PROSITE; PS00478; LIM_DOMAIN_1; 1.
DR PROSITE; PS50023; LIM_DOMAIN_2; 2.
PE 1: Evidence at protein level;
KW DNA-binding; Homeobox; LIM domain; Metal-binding; Nucleus;
KW Reference proteome; Repeat; Transcription; Transcription regulation; Zinc.
FT CHAIN 1..452
FT /note="LIM/homeobox protein lim-7"
FT /id="PRO_0000451292"
FT DOMAIN 54..116
FT /note="LIM zinc-binding 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00125"
FT DOMAIN 117..179
FT /note="LIM zinc-binding 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00125"
FT DNA_BIND 265..324
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 184..268
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 347..376
FT /note="LIM interaction domain (LID)"
FT /evidence="ECO:0000269|PubMed:28676648"
FT COMPBIAS 184..204
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 218..233
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 84..161
FT /note="Missing: In tm674; larval (L1 stage) lethality."
FT /evidence="ECO:0000269|PubMed:19116151"
SQ SEQUENCE 452 AA; 50486 MW; 1F12FF1A03BE1171 CRC64;
MNICMRNGYE QFSLTSPGTS DLEIGGSFWK DEPDTKYLCL DSPVEQRQHQ PPMAVCAGCR
LEISDRYFLR VNPNLEFHAQ CLKCVQCSRP LDENQTAFVK NGQTYCRDDY RRLFTTRCSR
CHGDFDKTDL VMRAGPQNVF HLNCFACVAC EKRLQTGEEF QIKNNSLYCR SDCRGLDNPD
TSASVPDYSK LNNNNNNDNN NSSSNFDEDE WDEERSTLTS LDNNTSSPLG SPKSDGVRTP
LFGHHNSGSG GSTSSCGKKK KDKQATRVRT VLNENQLKIL RDCYSINSRP DATLKERLVE
MTGLSARVIR VWFQNKRCKD KKRQIQITEN RLNSEREEVL NRVRVNGIGP LMVQPATPHI
DNTLGGPIDI QHFAQWNGTP PPPPPQYGNP MMFNSPSTFD VSVILAPVAP NVTSPSEALG
PLGASVFPHF SPQHAPFTAT SHDISSPAPC GE
