ID   LIMB_RHOER              Reviewed;         387 AA.
AC   Q9EUT9;
DT   10-MAY-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 65.
DE   RecName: Full=Limonene 1,2-monooxygenase;
DE            EC=1.14.13.107;
GN   Name=limB;
OS   Rhodococcus erythropolis (Arthrobacter picolinophilus).
OC   Bacteria; Actinobacteria; Corynebacteriales; Nocardiaceae; Rhodococcus;
OC   Rhodococcus erythropolis group.
OX   NCBI_TaxID=1833;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=DCL 14;
RA   Van der Vlugt-Bergmans C.J.B., van der Werf M.J.;
RT   "Genetic analysis of the lim operon of Rhodococcus erythropolis DCL14.";
RL   Submitted (JUL-2000) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   CHARACTERIZATION.
RX   PubMed=10224006; DOI=10.1128/aem.65.5.2092-2102.1999;
RA   van der Werf M.J., Swarts H.J., de Bont J.A.M.;
RT   "Rhodococcus erythropolis DCL14 contains a novel degradation pathway for
RT   limonene.";
RL   Appl. Environ. Microbiol. 65:2092-2102(1999).
CC   -!- FUNCTION: Acts on both enantiomers of limonene by their NAD-dependent
CC       epoxidation at the 1,2 double bond forming limonene-1,2-epoxide.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(4S)-limonene + H(+) + NADPH + O2 = H2O + limonene 1,2-epoxide
CC         + NADP(+); Xref=Rhea:RHEA:26085, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:15383,
CC         ChEBI:CHEBI:16431, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349;
CC         EC=1.14.13.107;
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(4S)-limonene + H(+) + NADH + O2 = H2O + limonene 1,2-epoxide
CC         + NAD(+); Xref=Rhea:RHEA:26089, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:15379, ChEBI:CHEBI:15383, ChEBI:CHEBI:16431,
CC         ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.14.13.107;
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(4R)-limonene + H(+) + NADH + O2 = H2O + limonene 1,2-epoxide
CC         + NAD(+); Xref=Rhea:RHEA:26093, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:15379, ChEBI:CHEBI:15382, ChEBI:CHEBI:16431,
CC         ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.14.13.107;
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(4R)-limonene + H(+) + NADPH + O2 = H2O + limonene 1,2-epoxide
CC         + NADP(+); Xref=Rhea:RHEA:26097, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:15382,
CC         ChEBI:CHEBI:16431, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349;
CC         EC=1.14.13.107;
CC   -!- COFACTOR:
CC       Name=FAD; Xref=ChEBI:CHEBI:57692;
CC   -!- PATHWAY: Terpene metabolism; (4R)-limonene degradation; (1S,4R)-1-
CC       hydroxylimonen-2-one from (4R)-limonene: step 1/3.
CC   -!- SIMILARITY: Belongs to the bacterial luciferase oxidoreductase family.
CC       {ECO:0000305}.
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DR   EMBL; AJ272366; CAC20855.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q9EUT9; -.
DR   SMR; Q9EUT9; -.
DR   KEGG; ag:CAC20855; -.
DR   BioCyc; MetaCyc:MON-13976; -.
DR   BRENDA; 1.14.13.107; 5389.
DR   UniPathway; UPA00987; UER00864.
DR   GO; GO:0106319; F:(R)-limonene 1,2-monooxygenase NADH activity; IEA:UniProtKB-EC.
DR   GO; GO:0106320; F:(R)-limonene 1,2-monooxygenase NADPH activity; IEA:UniProtKB-EC.
DR   GO; GO:0106324; F:(S)-limonene 1,2-monooxygenase NADH activity; IEA:RHEA.
DR   GO; GO:0106323; F:(S)-limonene 1,2-monooxygenase NADPH activity; IEA:RHEA.
DR   Gene3D; 3.20.20.30; -; 1.
DR   InterPro; IPR011251; Luciferase-like_dom.
DR   InterPro; IPR036661; Luciferase-like_sf.
DR   Pfam; PF00296; Bac_luciferase; 1.
DR   SUPFAM; SSF51679; SSF51679; 1.
PE   1: Evidence at protein level;
KW   FAD; Flavoprotein; Monooxygenase; NAD; NADP; Oxidoreductase.
FT   CHAIN           1..387
FT                   /note="Limonene 1,2-monooxygenase"
FT                   /id="PRO_0000220184"
SQ   SEQUENCE   387 AA;  42371 MW;  C3BD6B5849F2F08C CRC64;
     MTDDFGRVDF GAFLAPWHRA DSDANFAIHQ DLELVEHLDR LGFAEFWLGE HHSGGVEIVA
     SPEMFMAAAA QRTQRIKLGL GVVSLPYHHP FLVADRLVLL DHLSRGRMIF GAGPGQLADD
     AKMLGIDPID SRRKMEEAFD VIHRLLAGET VTQKTDWFTC QDAYLHVAPY SNIQKAVTAT
     VSPTGPKLAG KYGSGILSLA ATNPVGVEKL AEHWKIAEDI AAENGQTVDR ADWRLSGIMH
     VAETEEQARA DVRHGLLYLM NYLSNITPGF AAAPDVDSLI DGINDAGLAV IGTPEMAVTQ
     IRRLQEKSGG FGKFLVLHGE WASTTAALHS FELIAQQVAP HFNGDLGPRL RGYNQTMNSN
     RSAADITQAA QEEAQKRFEA ERAIRTN