LIMCH_DICDI
ID LIMCH_DICDI Reviewed; 686 AA.
AC Q55GV9;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 1.
DT 25-MAY-2022, entry version 95.
DE RecName: Full=Calponin homology and LIM domain-containing protein;
DE Short=CH-LIM;
GN Name=ChLim; ORFNames=DDB_G0267490;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [2]
RP FUNCTION, INDUCTION, AND INTERACTION WITH LIMF AND RAB21.
RX PubMed=15962002; DOI=10.1038/sj.emboj.7600716;
RA Khurana T., Brzostowski J.A., Kimmel A.R.;
RT "A Rab21/LIM-only/CH-LIM complex regulates phagocytosis via both activating
RT and inhibitory mechanisms.";
RL EMBO J. 24:2254-2264(2005).
CC -!- FUNCTION: Involved in the regulation of phagocytosis. May repress
CC rab21. {ECO:0000269|PubMed:15962002}.
CC -!- SUBUNIT: Interacts with limF and rab21. {ECO:0000269|PubMed:15962002}.
CC -!- INTERACTION:
CC Q55GV9; Q86I44: limF; NbExp=3; IntAct=EBI-1808948, EBI-1808928;
CC -!- INDUCTION: Repressed by limF. {ECO:0000269|PubMed:15962002}.
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DR EMBL; AAFI02000003; EAL73198.1; -; Genomic_DNA.
DR RefSeq; XP_647075.1; XM_641983.1.
DR AlphaFoldDB; Q55GV9; -.
DR SMR; Q55GV9; -.
DR IntAct; Q55GV9; 2.
DR STRING; 44689.DDB0231986; -.
DR PaxDb; Q55GV9; -.
DR EnsemblProtists; EAL73198; EAL73198; DDB_G0267490.
DR GeneID; 8615879; -.
DR KEGG; ddi:DDB_G0267490; -.
DR dictyBase; DDB_G0267490; ChLim.
DR eggNOG; KOG1703; Eukaryota.
DR eggNOG; KOG1704; Eukaryota.
DR HOGENOM; CLU_401396_0_0_1; -.
DR InParanoid; Q55GV9; -.
DR OMA; CEVISYV; -.
DR Reactome; R-DDI-6798695; Neutrophil degranulation.
DR PRO; PR:Q55GV9; -.
DR Proteomes; UP000002195; Chromosome 1.
DR GO; GO:0005938; C:cell cortex; IDA:dictyBase.
DR GO; GO:0030863; C:cortical cytoskeleton; IDA:dictyBase.
DR GO; GO:0005856; C:cytoskeleton; IBA:GO_Central.
DR GO; GO:0005829; C:cytosol; IDA:dictyBase.
DR GO; GO:0001891; C:phagocytic cup; IDA:dictyBase.
DR GO; GO:0045335; C:phagocytic vesicle; IDA:dictyBase.
DR GO; GO:0008270; F:zinc ion binding; ISS:dictyBase.
DR GO; GO:0046847; P:filopodium assembly; IMP:dictyBase.
DR GO; GO:0050765; P:negative regulation of phagocytosis; IMP:dictyBase.
DR GO; GO:0006909; P:phagocytosis; IEA:UniProtKB-KW.
DR CDD; cd00014; CH; 1.
DR Gene3D; 1.10.418.10; -; 1.
DR InterPro; IPR001715; CH-domain.
DR InterPro; IPR036872; CH_dom_sf.
DR InterPro; IPR037987; FHL2/3/5.
DR InterPro; IPR003096; SM22_calponin.
DR InterPro; IPR001781; Znf_LIM.
DR PANTHER; PTHR24205; PTHR24205; 3.
DR Pfam; PF00307; CH; 1.
DR Pfam; PF00412; LIM; 4.
DR PRINTS; PR00888; SM22CALPONIN.
DR SMART; SM00033; CH; 1.
DR SMART; SM00132; LIM; 6.
DR SUPFAM; SSF47576; SSF47576; 1.
DR PROSITE; PS50021; CH; 1.
DR PROSITE; PS00478; LIM_DOMAIN_1; 3.
DR PROSITE; PS50023; LIM_DOMAIN_2; 6.
PE 1: Evidence at protein level;
KW LIM domain; Metal-binding; Phagocytosis; Reference proteome; Repeat; Zinc.
FT CHAIN 1..686
FT /note="Calponin homology and LIM domain-containing protein"
FT /id="PRO_0000328170"
FT DOMAIN 15..120
FT /note="Calponin-homology (CH)"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00044"
FT DOMAIN 139..200
FT /note="LIM zinc-binding 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00125"
FT DOMAIN 219..279
FT /note="LIM zinc-binding 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00125"
FT DOMAIN 373..435
FT /note="LIM zinc-binding 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00125"
FT DOMAIN 437..495
FT /note="LIM zinc-binding 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00125"
FT DOMAIN 519..579
FT /note="LIM zinc-binding 5"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00125"
FT DOMAIN 583..658
FT /note="LIM zinc-binding 6"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00125"
FT REGION 305..345
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 323..339
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 686 AA; 76480 MW; 0EFAAD648D5D5AEC CRC64;
MIKANWTSTS AFNLELALDE SRDWIERVIN QKFPSDFQSS LRDGIFLCKL INQIQPNSVP
KYNQSPSTDF AKRENIQLFI KSAKHSMGLR DTQLFESQDL FESIRIRNIA ITLYWLGRAA
RASQTYKGPQ LDLLKFQGMN CSACKKAITN NDYLTTMTQQ FHTSCAVCCS CSCKLDPKKK
FYQESNNFWC ENCMLGATNL GGSNNSSGGK NKSNNNNNNK CSGCFGSLEK GYVPDENDKE
KKYCTSCICD LCHDPLIGNF QVKDGKKVCD SCSCKSCGKS LEDGYYEEGI SKYCEPCAKD
RNKPKQVMDK DGHDHHHHNH NKPTTTTTTT NSNSPLAKKK SDSCKMCDKP VDNKTKKYGD
DRDKYCTPHE KDGTCGKCNG ELVGSAISVM DKNFHPQCFK CDSCNKNLNQ NDQIKKSPTT
GNPLCGPCSS NNNKSSKNCH DCKKPISGSS VEALDRPYHP NCLKCYSCSK NLKEDFTEVD
NEPFCNPCAS QLNQYTSGNQ KQPKQGGSPF ITSGWLDSDR CVVCVKPLNG EVAKIFDSFY
HKGCFKCTDK SCNAPLLTGY FPHDKKPYCQ KCSIKIQQST TTDHCAKCSK PIIEGSILKV
AGKVYHKSCY DNEKHTSSSS SSSSVNCFKC KSQITGTQFV RLDQKDYCMK CSPSASSSTT
VTHGERLNYG MTVDPRSGKR VFNTSK
