ID   LIMC_DICDI              Reviewed;         182 AA.
AC   Q9BIW5; Q54YR2;
DT   08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2001, sequence version 1.
DT   25-MAY-2022, entry version 94.
DE   RecName: Full=LIM domain-containing protein C;
GN   Name=limC; ORFNames=DDB_G0277881;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION,
RP   DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RC   STRAIN=AX2;
RX   PubMed=12374734; DOI=10.1093/emboj/cdf550;
RA   Khurana B., Khurana T., Khaire N., Noegel A.A.;
RT   "Functions of LIM proteins in cell polarity and chemotactic motility.";
RL   EMBO J. 21:5331-5342(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
CC   -!- FUNCTION: Binds to F-actin and may modulate the chemotactic response
CC       during early development and contribute to the maintenance of the
CC       strength of the actin cytoskeleton. {ECO:0000269|PubMed:12374734}.
CC   -!- SUBCELLULAR LOCATION: Cell projection, pseudopodium
CC       {ECO:0000269|PubMed:12374734}. Cytoplasm, cell cortex
CC       {ECO:0000269|PubMed:12374734}. Cytoplasm, cytoskeleton
CC       {ECO:0000269|PubMed:12374734}.
CC   -!- DEVELOPMENTAL STAGE: Expressed during the growth phase and early
CC       development. Expression starts to decrease strongly after tight
CC       aggregate formation. {ECO:0000269|PubMed:12374734}.
CC   -!- DISRUPTION PHENOTYPE: Cells exhibit normal growth, but display altered
CC       morphology and F-actin distribution. They show changes in chemotactic
CC       motility associated with increased pseudopod formation, are
CC       significantly impaired in growth under stress conditions and highly
CC       sensitive to osmotic shock. {ECO:0000269|PubMed:12374734}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AF348466; AAK30152.1; -; Genomic_DNA.
DR   EMBL; AAFI02000023; EAL68113.1; -; Genomic_DNA.
DR   RefSeq; XP_642140.1; XM_637048.1.
DR   AlphaFoldDB; Q9BIW5; -.
DR   STRING; 44689.DDB0214931; -.
DR   PaxDb; Q9BIW5; -.
DR   EnsemblProtists; EAL68113; EAL68113; DDB_G0277881.
DR   GeneID; 8621348; -.
DR   KEGG; ddi:DDB_G0277881; -.
DR   dictyBase; DDB_G0277881; limC.
DR   eggNOG; KOG1700; Eukaryota.
DR   HOGENOM; CLU_054591_2_0_1; -.
DR   InParanoid; Q9BIW5; -.
DR   OMA; VQCEGHS; -.
DR   PhylomeDB; Q9BIW5; -.
DR   PRO; PR:Q9BIW5; -.
DR   Proteomes; UP000002195; Chromosome 3.
DR   GO; GO:0005938; C:cell cortex; IDA:dictyBase.
DR   GO; GO:0070382; C:exocytic vesicle; IDA:dictyBase.
DR   GO; GO:0031941; C:filamentous actin; IDA:dictyBase.
DR   GO; GO:0044354; C:macropinosome; IDA:dictyBase.
DR   GO; GO:0001891; C:phagocytic cup; IDA:dictyBase.
DR   GO; GO:0031143; C:pseudopodium; IDA:dictyBase.
DR   GO; GO:0051015; F:actin filament binding; IDA:dictyBase.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0006935; P:chemotaxis; IEA:UniProtKB-KW.
DR   GO; GO:0030866; P:cortical actin cytoskeleton organization; TAS:dictyBase.
DR   GO; GO:0006970; P:response to osmotic stress; IDA:dictyBase.
DR   InterPro; IPR001781; Znf_LIM.
DR   Pfam; PF00412; LIM; 2.
DR   SMART; SM00132; LIM; 2.
DR   PROSITE; PS00478; LIM_DOMAIN_1; 2.
DR   PROSITE; PS50023; LIM_DOMAIN_2; 2.
PE   2: Evidence at transcript level;
KW   Actin-binding; Cell projection; Chemotaxis; Cytoplasm; Cytoskeleton;
KW   LIM domain; Metal-binding; Reference proteome; Repeat; Zinc.
FT   CHAIN           1..182
FT                   /note="LIM domain-containing protein C"
FT                   /id="PRO_0000328166"
FT   DOMAIN          3..63
FT                   /note="LIM zinc-binding 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00125"
FT   DOMAIN          110..170
FT                   /note="LIM zinc-binding 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00125"
SQ   SEQUENCE   182 AA;  20555 MW;  00AF793E49EBDD9A CRC64;
     MSSICPTCTK RVYAAEAVKA CEKQYHKLCL QCFHCHKILQ LGQYSERDGQ PYCKTDYDRL
     FRQAGYRGGG VVADSFEPAP KVETTTPVEP TPPPTFLTPT EEVKVQLFPT NCPKCGKKAY
     FNELKVYNSR DWHKTCFACF SCNKNLVSGQ YSEKEGLIYC PRCYQSKFGP SGYTNTGALV
     LH