ID   LIMS2_BOVIN             Reviewed;         341 AA.
AC   Q2KJ33;
DT   12-DEC-2006, integrated into UniProtKB/Swiss-Prot.
DT   12-DEC-2006, sequence version 2.
DT   03-AUG-2022, entry version 102.
DE   RecName: Full=LIM and senescent cell antigen-like-containing domain protein 2;
DE   AltName: Full=Particularly interesting new Cys-His protein 2;
DE            Short=PINCH-2;
GN   Name=LIMS2; Synonyms=PINCH2;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Hereford; TISSUE=Ascending colon;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Adapter protein in a cytoplasmic complex linking beta-
CC       integrins to the actin cytoskeleton, bridges the complex to cell
CC       surface receptor tyrosine kinases and growth factor receptors.
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Interacts with integrin-linked protein kinase 1 (ILK) via the
CC       first LIM domain, and in competition with LIMS1. Part of the
CC       heterotrimeric IPP complex composed of integrin-linked kinase (ILK),
CC       LIMS1 or LIMS2, and PARVA. Interacts with TGFB1I1 (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell junction, focal adhesion {ECO:0000250}. Cell
CC       membrane {ECO:0000250}; Peripheral membrane protein {ECO:0000250};
CC       Cytoplasmic side {ECO:0000250}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; BC105544; AAI05545.2; -; mRNA.
DR   RefSeq; NP_001124223.1; NM_001130751.1.
DR   AlphaFoldDB; Q2KJ33; -.
DR   SMR; Q2KJ33; -.
DR   STRING; 9913.ENSBTAP00000008216; -.
DR   PaxDb; Q2KJ33; -.
DR   Ensembl; ENSBTAT00000008216; ENSBTAP00000008216; ENSBTAG00000006262.
DR   GeneID; 515401; -.
DR   KEGG; bta:515401; -.
DR   CTD; 55679; -.
DR   VEuPathDB; HostDB:ENSBTAG00000006262; -.
DR   VGNC; VGNC:30891; LIMS2.
DR   eggNOG; KOG2272; Eukaryota.
DR   GeneTree; ENSGT00940000153518; -.
DR   HOGENOM; CLU_001357_0_0_1; -.
DR   InParanoid; Q2KJ33; -.
DR   OMA; FHEHCFV; -.
DR   OrthoDB; 1593918at2759; -.
DR   TreeFam; TF314113; -.
DR   Proteomes; UP000009136; Chromosome 2.
DR   Bgee; ENSBTAG00000006262; Expressed in trachea and 104 other tissues.
DR   ExpressionAtlas; Q2KJ33; baseline and differential.
DR   GO; GO:0005911; C:cell-cell junction; IBA:GO_Central.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0005925; C:focal adhesion; IBA:GO_Central.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0098609; P:cell-cell adhesion; IBA:GO_Central.
DR   GO; GO:0045216; P:cell-cell junction organization; IBA:GO_Central.
DR   GO; GO:2001046; P:positive regulation of integrin-mediated signaling pathway; IBA:GO_Central.
DR   GO; GO:1900026; P:positive regulation of substrate adhesion-dependent cell spreading; IBA:GO_Central.
DR   InterPro; IPR017351; PINCH_1-like.
DR   InterPro; IPR001781; Znf_LIM.
DR   PANTHER; PTHR24210; PTHR24210; 1.
DR   Pfam; PF00412; LIM; 5.
DR   PIRSF; PIRSF038003; PINCH; 1.
DR   SMART; SM00132; LIM; 5.
DR   PROSITE; PS00478; LIM_DOMAIN_1; 4.
DR   PROSITE; PS50023; LIM_DOMAIN_2; 5.
PE   2: Evidence at transcript level;
KW   Cell junction; Cell membrane; LIM domain; Membrane; Metal-binding;
KW   Reference proteome; Repeat; Zinc.
FT   CHAIN           1..341
FT                   /note="LIM and senescent cell antigen-like-containing
FT                   domain protein 2"
FT                   /id="PRO_0000266010"
FT   DOMAIN          13..74
FT                   /note="LIM zinc-binding 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00125"
FT   DOMAIN          76..133
FT                   /note="LIM zinc-binding 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00125"
FT   DOMAIN          138..195
FT                   /note="LIM zinc-binding 3"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00125"
FT   DOMAIN          196..255
FT                   /note="LIM zinc-binding 4"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00125"
FT   DOMAIN          256..315
FT                   /note="LIM zinc-binding 5"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00125"
SQ   SEQUENCE   341 AA;  38895 MW;  D7B217FCC8D6792B CRC64;
     MTGSNMSNAL ANAVCQRCQA RFAPAERIVN SNGELYHEHC FVCAQCFRPF PEGLFYEFEG
     RKYCEHDFQM LFAPCCGSCG EFIIGRVIKA MNNNWHPGCF RCELCDVELA DLGFVKNAGR
     HLCRPCHNRE KAKGLGKYIC QRCHLVIDEQ PLMFKNDAYH PDHFSCTHCG KELTAEAREL
     KGELYCLPCH DKMGVPICGA CRRPIEGRVV NALGKQWHVE HFVCAKCEKP FLGHRHYEKK
     GLAYCETHYN QLFGDVCYTC SHVIEGDVVS ALNKAWCVHC FSCSTCNSRL TLKNKFVEFD
     MKPVCKRCYE KFPLELKKRL KKLSELAARR AQPKSSGLHP A