ID   LIN1_NYCCO              Reviewed;        1260 AA.
AC   P08548;
DT   01-AUG-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1988, sequence version 1.
DT   03-AUG-2022, entry version 78.
DE   RecName: Full=LINE-1 reverse transcriptase homolog;
DE            EC=2.7.7.49;
OS   Nycticebus coucang (Slow loris).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Strepsirrhini; Lorisiformes;
OC   Lorisidae; Nycticebus.
OX   NCBI_TaxID=9470;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2423883; DOI=10.1038/321625a0;
RA   Hattori M., Kuhara S., Takenaka O., Sakaki Y.;
RT   "L1 family of repetitive DNA sequences in primates may be derived from a
RT   sequence encoding a reverse transcriptase-related protein.";
RL   Nature 321:625-628(1986).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC         diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC         Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC         ChEBI:CHEBI:173112; EC=2.7.7.49; Evidence={ECO:0000255|PROSITE-
CC         ProRule:PRU00405};
CC   -!- MISCELLANEOUS: This sequence was constructed from an alignment of six
CC       sequences, determined by these authors but not shown, belonging to the
CC       LINE-1 family.
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DR   PIR; B25313; GNLRL1.
DR   AlphaFoldDB; P08548; -.
DR   SMR; P08548; -.
DR   PRIDE; P08548; -.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0003964; F:RNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR   Gene3D; 3.60.10.10; -; 1.
DR   InterPro; IPR043502; DNA/RNA_pol_sf.
DR   InterPro; IPR013544; DUF1725.
DR   InterPro; IPR036691; Endo/exonu/phosph_ase_sf.
DR   InterPro; IPR005135; Endo/exonuclease/phosphatase.
DR   InterPro; IPR000477; RT_dom.
DR   Pfam; PF08333; DUF1725; 1.
DR   Pfam; PF03372; Exo_endo_phos; 1.
DR   Pfam; PF00078; RVT_1; 1.
DR   SUPFAM; SSF56219; SSF56219; 1.
DR   SUPFAM; SSF56672; SSF56672; 1.
DR   PROSITE; PS50878; RT_POL; 1.
PE   4: Predicted;
KW   Magnesium; Metal-binding; Nucleotidyltransferase;
KW   RNA-directed DNA polymerase; Transferase.
FT   CHAIN           1..1260
FT                   /note="LINE-1 reverse transcriptase homolog"
FT                   /id="PRO_0000084427"
FT   DOMAIN          497..772
FT                   /note="Reverse transcriptase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00405"
FT   DOMAIN          1242..1260
FT                   /note="DUF1725"
FT   BINDING         599
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00405"
FT   BINDING         701
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00405"
FT   BINDING         702
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /ligand_note="catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00405"
SQ   SEQUENCE   1260 AA;  147043 MW;  7A6803DF471F7253 CRC64;
     TGLSKGLSIF SINVNGLNCP LKRHRLADWI QKLKPDICCI QESHLTLKDK YRLKVKGWSS
     IFQANGKQKK AGIAILFADA IGFKPTKIRK DKDGHFIFVK GNTQYDEISI INIYAPNHNA
     PQFIRETLTD MSNLISSTSI VVGDFNTPLA VLDRSSKKKL SKEILDLNST IQHLDLTDIY
     RTFHPNKTEY TFFSSAHGTY SKIDHILGHK SNLSKFKKIE IIPCIFSDHH GIKVELNNNR
     NLHTHTKTWK LNNLMLKDTW VIDEIKKEIT KFLEQNNNQD TNYQNLWDTA KAVLRGKFIA
     LQAFLKKTER EEVNNLMGHL KQLEKEEHSN PKPSRRKEIT KIRAELNEIE NKRIIQQINK
     SKSWFFEKIN KIDKPLANLT RKKRVKSLIS SIRNGNDEIT TDPSEIQKIL NEYYKKLYSH
     KYENLKEIDQ YLEACHLPRL SQKEVEMLNR PISSSEIAST IQNLPKKKSP GPDGFTSEFY
     QTFKEELVPI LLNLFQNIEK EGILPNTFYE ANITLIPKPG KDPTRKENYR PISLMNIDAK
     ILNKILTNRI QQHIKKIIHH DQVGFIPGSQ GWFNIRKSIN VIQHINKLKN KDHMILSIDA
     EKAFDNIQHP FMIRTLKKIG IEGTFLKLIE AIYSKPTANI ILNGVKLKSF PLRSGTRQGC
     PLSPLLFNIV MEVLAIAIRE EKAIKGIHIG SEEIKLSLFA DDMIVYLENT RDSTTKLLEV
     IKEYSNVSGY KINTHKSVAF IYTNNNQAEK TVKDSIPFTV VPKKMKYLGV YLTKDVKDLY
     KENYETLRKE IAEDVNKWKN IPCSWLGRIN IVKMSILPKA IYNFNAIPIK APLSYFKDLE
     KIILHFIWNQ KKPQIAKTLL SNKNKAGGIT LPDLRLYYKS IVIKTAWYWH KNREVDVWNR
     IENQEMDPAT YHYLIFDKPI KNIQWGKDSL FNKWCWVNWL AICRRLKLDP HLSPLTKIDS
     HWIKDLNLRH ETIKILEESA GKTLEGISLG EYFMRRTPQA IEAVSKIHYW DLIKLKSFCT
     AKNIVSKASR QPSEWEKIFA GYTSDKGLIT RIHRELKHIN KKRTRDPISG WARDLKRNFS
     KEDRHTIYKH MKKSSSSLII REMQIKTTLR YHLTPVRVAH ITKSPNQRCW RGCGGKGTLL
     HCWWECPLIR SFWKDVWRIL RDLKIDLPFD PIIPLLGLYP EDQKSQYNKD ICTRMFIAAQ
     FIIAKSWKKP KCPSTHEWTS KLWYMYTMEY YAALKKDGDF TSFMFTWMEL EHILLSKVSQ