LIN22_CAEEL
ID LIN22_CAEEL Reviewed; 173 AA.
AC G5EF76;
DT 07-APR-2021, integrated into UniProtKB/Swiss-Prot.
DT 14-DEC-2011, sequence version 1.
DT 03-AUG-2022, entry version 92.
DE RecName: Full=Helix-loop-helix protein lin-22 {ECO:0000305};
DE AltName: Full=Lineage abnormal protein 22 {ECO:0000312|WormBase:Y54G2A.1};
GN Name=lin-22 {ECO:0000312|WormBase:Y54G2A.1};
GN Synonyms=him-5 {ECO:0000312|EMBL:AAB68848.1},
GN hlh-9 {ECO:0000312|WormBase:Y54G2A.1};
GN ORFNames=Y54G2A.1 {ECO:0000312|WormBase:Y54G2A.1};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN [1] {ECO:0000312|EMBL:AAB68848.1}
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND MUTAGENESIS OF GLU-30.
RX PubMed=9247331; DOI=10.1242/dev.124.15.2875;
RA Wrischnik L.A., Kenyon C.J.;
RT "The role of lin-22, a hairy/enhancer of split homolog, in patterning the
RT peripheral nervous system of C. elegans.";
RL Development 124:2875-2888(1997).
RN [2] {ECO:0000312|Proteomes:UP000001940}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [3] {ECO:0000305}
RP FUNCTION, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=29108019; DOI=10.1371/journal.pbio.2002429;
RA Katsanos D., Koneru S.L., Mestek Boukhibar L., Gritti N., Ghose R.,
RA Appleford P.J., Doitsidou M., Woollard A., van Zon J.S., Poole R.J.,
RA Barkoulas M.;
RT "Stochastic loss and gain of symmetric divisions in the C. elegans
RT epidermis perturbs robustness of stem cell number.";
RL PLoS Biol. 15:e2002429-e2002429(2017).
CC -!- FUNCTION: Probable transcription factor (PubMed:9247331,
CC PubMed:29108019). During development, required for cell fate
CC specification, probably by promoting or repressing expression of genes
CC involved in specific cell fate (PubMed:9247331, PubMed:29108019).
CC Involved in specifying lineages derived from the epidermal stem cells
CC of the lateral ectoderm, known as seam cells (PubMed:9247331,
CC PubMed:29108019). Modulates symmetric divisions of seam cells, perhaps
CC in concert with the Wnt signaling pathway (PubMed:29108019). May
CC repress expression of homeobox genes mab-5, egl-5 and lin-39
CC (PubMed:9247331). {ECO:0000269|PubMed:29108019,
CC ECO:0000269|PubMed:9247331}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00981}.
CC -!- TISSUE SPECIFICITY: Expressed mostly in the seam (stem) cells and
CC hypodermis (hyp7), but also to a lesser extent in the intestine.
CC {ECO:0000269|PubMed:29108019}.
CC -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown leads to an increase in
CC seam (stem) cell number variability. {ECO:0000269|PubMed:29108019}.
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DR EMBL; AF020555; AAB68848.1; -; mRNA.
DR EMBL; BX284604; CCD83486.1; -; Genomic_DNA.
DR PIR; T42235; T42235.
DR RefSeq; NP_500281.1; NM_067880.3.
DR AlphaFoldDB; G5EF76; -.
DR SMR; G5EF76; -.
DR STRING; 6239.Y54G2A.1; -.
DR PaxDb; G5EF76; -.
DR EnsemblMetazoa; Y54G2A.1.1; Y54G2A.1.1; WBGene00003008.
DR GeneID; 177082; -.
DR KEGG; cel:CELE_Y54G2A.1; -.
DR CTD; 177082; -.
DR WormBase; Y54G2A.1; CE26703; WBGene00003008; lin-22.
DR eggNOG; KOG4304; Eukaryota.
DR HOGENOM; CLU_1588040_0_0_1; -.
DR InParanoid; G5EF76; -.
DR OMA; RSAQPCS; -.
DR OrthoDB; 1600748at2759; -.
DR Proteomes; UP000001940; Chromosome IV.
DR Bgee; WBGene00003008; Expressed in epithelium and 25 other tissues.
DR GO; GO:0005634; C:nucleus; ISS:WormBase.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; ISS:WormBase.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0009952; P:anterior/posterior pattern specification; IMP:WormBase.
DR GO; GO:0009957; P:epidermal cell fate specification; IMP:UniProtKB.
DR GO; GO:0010629; P:negative regulation of gene expression; IMP:WormBase.
DR GO; GO:0045138; P:nematode male tail tip morphogenesis; IMP:WormBase.
DR GO; GO:0050767; P:regulation of neurogenesis; IBA:GO_Central.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR Gene3D; 4.10.280.10; -; 1.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR036638; HLH_DNA-bd_sf.
DR Pfam; PF00010; HLH; 1.
DR SMART; SM00353; HLH; 1.
DR SUPFAM; SSF47459; SSF47459; 1.
DR PROSITE; PS50888; BHLH; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..173
FT /note="Helix-loop-helix protein lin-22"
FT /id="PRO_0000452407"
FT DOMAIN 21..78
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 21..34
FT /note="Basic motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 35..78
FT /note="Helix-loop-helix motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 83..102
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 83..99
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 30
FT /note="E->K: In n372; alters cell fate specification within
FT the V5 lineage, and also causes anterior V cells to
FT generate V5-like lineages, including ectopic formation of
FT neuronal structures called postdeirids. Ectopic postdeirids
FT are not formed on a lin-32 mutant background."
FT /evidence="ECO:0000269|PubMed:9247331"
SQ SEQUENCE 173 AA; 19543 MW; B0CAE3F3760FFAED CRC64;
MTSFLCSDTE IESDGGISRC KKIKNKPLME KKRRARINKS LSQLKQILIQ DEHKNSIQHS
KWEKADILEM AVEYLQQLRS AQPCSLSPST SSISTPPTPK EEIRNIKVPL NPIASFLNPM
MQQYVAYQQL AQLSMYTQLF NNPAGVPLRA DAGVTAQSPE LAEKLKIEDR SRV
