LIN25_CAEEL
ID LIN25_CAEEL Reviewed; 1139 AA.
AC Q10573; Q7JLH0;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 03-AUG-2022, entry version 106.
DE RecName: Full=Protein lin-25;
DE AltName: Full=Abnormal cell lineage protein 25;
GN Name=lin-25; ORFNames=F56H9.5;
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND MUTAGENESIS OF THR-840.
RC STRAIN=Bristol N2;
RX PubMed=7867931; DOI=10.1101/gad.9.3.341;
RA Tuck S., Greenwald I.;
RT "lin-25, a gene required for vulval induction in Caenorhabditis elegans.";
RL Genes Dev. 9:341-357(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [3]
RP FUNCTION, AND MUTAGENESIS OF PRO-751.
RX PubMed=3996896; DOI=10.1093/genetics/110.1.17;
RA Ferguson E.L., Horvitz H.R.;
RT "Identification and characterization of 22 genes that affect the vulval
RT cell lineages of the nematode Caenorhabditis elegans.";
RL Genetics 110:17-72(1985).
RN [4]
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND DEVELOPMENTAL
RP STAGE.
RX PubMed=9806929; DOI=10.1242/dev.125.23.4809;
RA Nilsson L., Li X., Tiensuu T., Auty R., Greenwald I., Tuck S.;
RT "Caenorhabditis elegans lin-25: cellular focus, protein expression and
RT requirement for sur-2 during induction of vulval fates.";
RL Development 125:4809-4819(1998).
RN [5]
RP FUNCTION, AND MUTAGENESIS OF CYS-103 AND 695-ILE--PRO-701.
RX PubMed=11063686; DOI=10.1093/genetics/156.3.1083;
RA Nilsson L., Tiensuu T., Tuck S.;
RT "Caenorhabditis elegans lin-25: a study of its role in multiple cell fate
RT specification events involving ras and the identification and
RT characterization of evolutionarily conserved domains.";
RL Genetics 156:1083-1096(2000).
RN [6]
RP FUNCTION.
RX PubMed=15268855; DOI=10.1016/j.cub.2004.07.022;
RA Nicholas H.R., Hodgkin J.;
RT "The ERK MAP kinase cascade mediates tail swelling and a protective
RT response to rectal infection in C. elegans.";
RL Curr. Biol. 14:1256-1261(2004).
CC -!- FUNCTION: Participates in the inductive signaling pathway downstream of
CC let-60 Ras and the RAF/MAP kinase cascade to regulate specification and
CC differentiation of many cell types (PubMed:11063686). Positively
CC regulates the fate of vulval precursor cells (PubMed:3996896,
CC PubMed:7867931, PubMed:9806929). Required for induction of the P12 and
CC excretory duct cell fates. In males, it is also required for proper
CC formation of spicules. Does not function in the signaling pathway that
CC promotes exit from pachytene (PubMed:11063686). Plays a role in
CC responses to M.nematophilum-mediated bacterial infection by promoting
CC tail swelling and preventing constipation (PubMed:15268855).
CC {ECO:0000269|PubMed:11063686, ECO:0000269|PubMed:15268855,
CC ECO:0000269|PubMed:3996896, ECO:0000269|PubMed:7867931,
CC ECO:0000269|PubMed:9806929}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:9806929}. Cytoplasm
CC {ECO:0000269|PubMed:9806929}.
CC -!- TISSUE SPECIFICITY: Expressed in seam cells and all six vulva precursor
CC cells (VPC). After VPC division, expression is restricted to
CC descendants of the VPC cell lineages P5.p, P6.p and P7.p (at protein
CC level). {ECO:0000269|PubMed:9806929}.
CC -!- DEVELOPMENTAL STAGE: Expressed in all stages but highest during L1, L2
CC and L3 stages (at protein level). {ECO:0000269|PubMed:9806929}.
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DR EMBL; U20168; AAA66364.1; -; mRNA.
DR EMBL; Z74473; CAA98951.1; -; Genomic_DNA.
DR EMBL; Z74476; CAA98951.1; JOINED; Genomic_DNA.
DR PIR; T22811; T22811.
DR RefSeq; NP_506162.1; NM_073761.6.
DR AlphaFoldDB; Q10573; -.
DR BioGRID; 44752; 11.
DR IntAct; Q10573; 1.
DR STRING; 6239.F56H9.5; -.
DR iPTMnet; Q10573; -.
DR EPD; Q10573; -.
DR PaxDb; Q10573; -.
DR PeptideAtlas; Q10573; -.
DR EnsemblMetazoa; F56H9.5.1; F56H9.5.1; WBGene00003011.
DR GeneID; 179732; -.
DR KEGG; cel:CELE_F56H9.5; -.
DR UCSC; F56H9.5; c. elegans.
DR CTD; 179732; -.
DR WormBase; F56H9.5; CE05975; WBGene00003011; lin-25.
DR eggNOG; ENOG502S9A0; Eukaryota.
DR HOGENOM; CLU_301410_0_0_1; -.
DR InParanoid; Q10573; -.
DR OMA; HCPLPRI; -.
DR OrthoDB; 739187at2759; -.
DR SignaLink; Q10573; -.
DR PRO; PR:Q10573; -.
DR Proteomes; UP000001940; Chromosome V.
DR Bgee; WBGene00003011; Expressed in embryo and 4 other tissues.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0001708; P:cell fate specification; IMP:UniProtKB.
DR GO; GO:0050830; P:defense response to Gram-positive bacterium; IMP:UniProtKB.
DR GO; GO:0002119; P:nematode larval development; IMP:WormBase.
DR GO; GO:0040025; P:vulval development; IMP:WormBase.
PE 1: Evidence at protein level;
KW Cytoplasm; Developmental protein; Differentiation; Nucleus;
KW Reference proteome.
FT CHAIN 1..1139
FT /note="Protein lin-25"
FT /id="PRO_0000084428"
FT MOTIF 695..701
FT /note="Nuclear localization signal"
FT MUTAGEN 103
FT /note="C->Y: In sy29; egg-laying defect."
FT /evidence="ECO:0000269|PubMed:11063686"
FT MUTAGEN 695..701
FT /note="Missing: Disrupts nuclear translocation."
FT /evidence="ECO:0000269|PubMed:11063686"
FT MUTAGEN 751
FT /note="P->L: In n545; many defects in reproductive organs;
FT egg-laying defect, sterile, protruding vulva, vulvaless and
FT male mating defect."
FT /evidence="ECO:0000269|PubMed:3996896"
FT MUTAGEN 840
FT /note="T->I: In n1772; temperature-sensitive."
FT /evidence="ECO:0000269|PubMed:7867931"
SQ SEQUENCE 1139 AA; 131217 MW; 74180F4ACEC7584E CRC64;
MNSIKIDSST KIVIEYIDKL RNCSNEKRQK VSSVIRPVDF PGISTQQLCD ILIRFAYNGT
YIDDVFRDHL LKLVVDKSIT WNQVIHSVIN TKTDRIYSKC LQCELIREMV NYVQIKSFSD
KLHADELISI MKPTVFFLAQ LIQDVLSDED ELEMIQIEYG DVRKAYYKPL EALSAFIHDD
LCSSLLAFSE ASEITHQLEL CRDSFSKLRS PDKSATTLVE MLIERHIEKC KPVKFQYIPE
GIALFDLKNP SIRILIPIFA CFKNHESSKL MAETIQTFAE IMRFYGPDVI FDILHGAILL
KCEESMDLLH FPKQHRADFR WQSTTFFYKK LPQIIEYLIR SEKLTVAEVQ FGLEKALTDL
SMMFDAADVA WQNASFLTLL NELEPVLGKT ITDPLRLRRR EHMKTNDQLL PFADTDNQLI
ENTDIDKLLN AVKEVMNLQF GQTEEFSKLF VDKVKTGQTD NFDAITSILI TEGRLMEVGK
AFVLKNVEAQ RSSAVGVNER IQIFDDTFIL LSRILIKNPS VSINMFVNGG PGKSDAEQTM
FYKWSMRYVK RVAKHRDPEP KDETEYIALR KEVEMLMRLA NAEVGIEDDL ENDVEEEMPE
VQEPNIETQN VDEISIPDPL HLLIENDPTV TAESKETEEV KFNPEGVEQM DTNEKPAEIS
DSLIEPTLLE EPKDILENII VPIIKVEAPK PDSPIKKKKD PDVTWHEVHC PLPRISRRTA
RAYLAQMKEG IPFWKTDDPN LNIGSILAAI PRLGQLLVDE HEEKRNRIDR KSAEENMTNI
LHAIESMPSL FLCLLEWLDC EPESGARTSL AFTINLSLER YLAVSTHGIN YMKWIFIKST
VQQMIDELVD KSPAFPEVTC TAFSTARRFC PFVGRNENPD QLKLKHAWYY MRQQAWASPH
ALRLLEHANT AREYDVWSHI YISKTIKSGC GDIMTSSVDM IFSFLMLDDL NSIIRIHESL
MAFWLSEDAG QCQADGRFDP LSIRVVIRLM TQVLLIAEWT LDRLLNEDPA FVERLNLKEV
KAPEPEDPDK REKWIFLLRS MLERTINRFF KIVRKGLLSN VVNTIIQLLK SIAGSADCKA
KRLLVKRIPP DLIFQLAYIE PSSVDYSLMN IYCDPDNKEH TQTKIMFLCA LRRSQSFSF
