LIN26_CAEEL
ID LIN26_CAEEL Reviewed; 490 AA.
AC Q27355; A0A2C9C321; Q9U5Z4; U4PC59; U4PFD0;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 26-FEB-2020, sequence version 3.
DT 03-AUG-2022, entry version 147.
DE RecName: Full=Transcription factor lin-26;
DE AltName: Full=Abnormal cell lineage protein 26;
GN Name=lin-26 {ECO:0000312|WormBase:F18A1.2d};
GN ORFNames=F18A1.2 {ECO:0000312|WormBase:F18A1.2d};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM C), FUNCTION, TISSUE SPECIFICITY, AND
RP MUTAGENESIS OF GLY-368; SER-369 AND LEU-373.
RC STRAIN=Bristol N2;
RX PubMed=7956818; DOI=10.1242/dev.120.9.2359;
RA Labouesse M., Sookhareea S., Horvitz H.R.;
RT "The Caenorhabditis elegans gene lin-26 is required to specify the fates of
RT hypodermal cells and encodes a presumptive zinc-finger transcription
RT factor.";
RL Development 120:2359-2368(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), TISSUE SPECIFICITY, AND
RP DEVELOPMENTAL STAGE.
RC STRAIN=Bristol N2;
RX PubMed=10224256; DOI=10.1093/genetics/152.1.221;
RA Dufourcq P., Chanal P., Vicaire S., Camut E., Quintin S., den Boer B.B.W.,
RA Bosher J.M., Labouesse M.;
RT "lir-2, lir-1 and lin-26 encode a new class of zinc-finger proteins and are
RT organized in two overlapping operons both in Caenorhabditis elegans and in
RT Caenorhabditis briggsae.";
RL Genetics 152:221-235(1999).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [4]
RP FUNCTION.
RX PubMed=11063687; DOI=10.1093/genetics/156.3.1097;
RA Eisenmann D.M., Kim S.K.;
RT "Protruding vulva mutants identify novel loci and Wnt signaling factors
RT that function during Caenorhabditis elegans vulva development.";
RL Genetics 156:1097-1116(2000).
RN [5] {ECO:0000305}
RP FUNCTION, DISRUPTION PHENOTYPE, AND MUTAGENESIS OF LEU-373.
RX PubMed=24885717; DOI=10.1186/1471-213x-14-17;
RA Liu W.J., Reece-Hoyes J.S., Walhout A.J., Eisenmann D.M.;
RT "Multiple transcription factors directly regulate Hox gene lin-39
RT expression in ventral hypodermal cells of the C. elegans embryo and larva,
RT including the hypodermal fate regulators LIN-26 and ELT-6.";
RL BMC Dev. Biol. 14:17-17(2014).
RN [6]
RP DISRUPTION PHENOTYPE.
RX PubMed=30921322; DOI=10.1371/journal.pgen.1008004;
RA Billmyre K.K., Doebley A.L., Spichal M., Heestand B., Belicard T.,
RA Sato-Carlton A., Flibotte S., Simon M., Gnazzo M., Skop A., Moerman D.,
RA Carlton P.M., Sarkies P., Ahmed S.;
RT "The meiotic phosphatase GSP-2/PP1 promotes germline immortality and small
RT RNA-mediated genome silencing.";
RL PLoS Genet. 15:E1008004-E1008004(2019).
CC -!- FUNCTION: Probable transcription factor (PubMed:7956818,
CC PubMed:24885717). Required to specify the fates of hypodermal and
CC neuron-associated support cells (PubMed:7956818). Functions during
CC vulval development, playing a role in vulval precursor cell fate
CC specification (PubMed:11063687, PubMed:24885717). Positively modulates
CC expression of homeobox protein lin-39, perhaps by binding to regulatory
CC regions of the lin-39 gene, acting in the vulval lineage
CC (PubMed:24885717). {ECO:0000269|PubMed:11063687,
CC ECO:0000269|PubMed:24885717, ECO:0000269|PubMed:7956818}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=4;
CC Name=d {ECO:0000312|WormBase:F18A1.2d};
CC IsoId=Q27355-1; Sequence=Displayed;
CC Name=a {ECO:0000312|WormBase:F18A1.2a}; Synonyms=lin-26B
CC {ECO:0000303|PubMed:10224256};
CC IsoId=Q27355-2; Sequence=VSP_060480;
CC Name=b {ECO:0000312|WormBase:F18A1.2b}; Synonyms=lin-26C
CC {ECO:0000303|PubMed:10224256};
CC IsoId=Q27355-3; Sequence=VSP_060480, VSP_060482, VSP_060483;
CC Name=c {ECO:0000312|WormBase:F18A1.2c}; Synonyms=lin-26C
CC {ECO:0000303|PubMed:10224256, ECO:0000303|PubMed:7956818};
CC IsoId=Q27355-4; Sequence=VSP_060481;
CC -!- TISSUE SPECIFICITY: Expressed in somatic gonads and germline precursors
CC until the 50-cell stage. After the 100-cell stage, expression is seen
CC in differentiating hypodermal and support cells (at protein level).
CC {ECO:0000269|PubMed:10224256, ECO:0000269|PubMed:7956818}.
CC -!- DEVELOPMENTAL STAGE: Expressed throughout development from embryos to
CC adults. {ECO:0000269|PubMed:10224256}.
CC -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown results in embryonic
CC lethality (PubMed:30921322). This is rescued by RNAi-mediated knockdown
CC of rsd-6, and nrde-2 (PubMed:30921322). RNAi-mediated knockdown at the
CC larval L1 stage causes a weak but significant decrease in expression of
CC lin-39 (PubMed:24885717). Knockdown in mothers caused lower levels of
CC lin-39 expression in the P blastomere of embryos (PubMed:24885717).
CC Knockdown in L1 stage larvae, in a lin-39 mutant background, causes
CC abnormal fusion of vulval precursor cells at larval stage L2
CC (PubMed:24885717). {ECO:0000269|PubMed:24885717,
CC ECO:0000269|PubMed:30921322}.
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DR EMBL; Z32673; CAA83585.1; -; mRNA.
DR EMBL; AJ130957; CAA10262.1; -; mRNA.
DR EMBL; BX284602; CCD62034.1; -; Genomic_DNA.
DR EMBL; BX284602; CDH93451.1; -; Genomic_DNA.
DR EMBL; BX284602; CDH93452.1; -; Genomic_DNA.
DR EMBL; BX284602; SOF58752.1; -; Genomic_DNA.
DR PIR; T34222; T34222.
DR RefSeq; NP_001022092.1; NM_001026921.2. [Q27355-2]
DR RefSeq; NP_001293586.1; NM_001306657.1. [Q27355-3]
DR RefSeq; NP_001293587.1; NM_001306658.1. [Q27355-4]
DR AlphaFoldDB; Q27355; -.
DR BioGRID; 532369; 2.
DR IntAct; Q27355; 5.
DR STRING; 6239.F18A1.2c; -.
DR EPD; Q27355; -.
DR PaxDb; Q27355; -.
DR PeptideAtlas; Q27355; -.
DR PRIDE; Q27355; -.
DR EnsemblMetazoa; F18A1.2a.1; F18A1.2a.1; WBGene00003012. [Q27355-2]
DR EnsemblMetazoa; F18A1.2b.1; F18A1.2b.1; WBGene00003012. [Q27355-3]
DR EnsemblMetazoa; F18A1.2c.1; F18A1.2c.1; WBGene00003012. [Q27355-4]
DR EnsemblMetazoa; F18A1.2d.1; F18A1.2d.1; WBGene00003012. [Q27355-1]
DR GeneID; 3565051; -.
DR UCSC; F18A1.2.2; c. elegans. [Q27355-1]
DR CTD; 3565051; -.
DR WormBase; F18A1.2a; CE27972; WBGene00003012; lin-26. [Q27355-2]
DR WormBase; F18A1.2b; CE48778; WBGene00003012; lin-26. [Q27355-3]
DR WormBase; F18A1.2c; CE04402; WBGene00003012; lin-26. [Q27355-4]
DR WormBase; F18A1.2d; CE52355; WBGene00003012; lin-26. [Q27355-1]
DR eggNOG; ENOG502TGSS; Eukaryota.
DR GeneTree; ENSGT00940000174779; -.
DR HOGENOM; CLU_046043_0_0_1; -.
DR InParanoid; Q27355; -.
DR OMA; DAHDITT; -.
DR OrthoDB; 1006660at2759; -.
DR SignaLink; Q27355; -.
DR PRO; PR:Q27355; -.
DR Proteomes; UP000001940; Chromosome II.
DR Bgee; WBGene00003012; Expressed in embryo and 4 other tissues.
DR GO; GO:0005634; C:nucleus; IDA:WormBase.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:WormBase.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IDA:WormBase.
DR GO; GO:0007398; P:ectoderm development; IMP:WormBase.
DR GO; GO:0008544; P:epidermis development; IMP:WormBase.
DR GO; GO:0045197; P:establishment or maintenance of epithelial cell apical/basal polarity; IMP:WormBase.
DR GO; GO:0008406; P:gonad development; IMP:WormBase.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IMP:WormBase.
DR GO; GO:0042659; P:regulation of cell fate specification; IMP:WormBase.
DR GO; GO:0000003; P:reproduction; IMP:WormBase.
DR GO; GO:0040025; P:vulval development; IMP:WormBase.
PE 1: Evidence at protein level;
KW Alternative splicing; DNA-binding; Metal-binding; Nucleus;
KW Reference proteome; Repeat; Transcription; Transcription regulation; Zinc;
KW Zinc-finger.
FT CHAIN 1..490
FT /note="Transcription factor lin-26"
FT /id="PRO_0000084429"
FT ZN_FING 353..381
FT /note="C2H2-type; degenerate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT REGION 96..176
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 101..110
FT /note="PEST"
FT /evidence="ECO:0000303|PubMed:7956818"
FT REGION 236..262
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 302..326
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 99..176
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 236..261
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..52
FT /note="Missing (in isoform a and isoform b)"
FT /evidence="ECO:0000305"
FT /id="VSP_060480"
FT VAR_SEQ 35..52
FT /note="Missing (in isoform c)"
FT /evidence="ECO:0000305"
FT /id="VSP_060481"
FT VAR_SEQ 389..410
FT /note="CWDCDFTGIKSNVISHARQCRH -> YRRDACRMESPPSRMLPRLCAC (in
FT isoform b)"
FT /evidence="ECO:0000305"
FT /id="VSP_060482"
FT VAR_SEQ 411..490
FT /note="Missing (in isoform b)"
FT /evidence="ECO:0000305"
FT /id="VSP_060483"
FT MUTAGEN 368
FT /note="G->E: In lin-26(mc1); embryonic lethality and the
FT death of many hypodermal cells."
FT /evidence="ECO:0000269|PubMed:7956818"
FT MUTAGEN 369
FT /note="S->F: In lin-26(mc2); embryonic lethality due to a
FT failure of hypodermal cells to enclose the embryo."
FT /evidence="ECO:0000269|PubMed:7956818"
FT MUTAGEN 373
FT /note="L->F: In lin-26(n156); hypodermal Pn.p cells express
FT a neural fate; animals lack a vulva. Shows a small but
FT significant decrease in lin-39 at the early larval L3
FT stage."
FT /evidence="ECO:0000269|PubMed:7956818"
SQ SEQUENCE 490 AA; 55271 MW; 2B063C96EDF4CACC CRC64;
MILHGFHLST SSHCLPFIFR HLFIFHSITA LCDHVISNYC NSLKLSNNKF NQMLSKFVVV
EVSNSNNTMT LVEYLVTHGF DKFAVVTDLV DHPSFKYKDG SSSPESPSTT ASTAAQHTPP
RTAVSTPTSI NTPVPPHQNK QRHSIDKIAA NLSTKKVSPS SIEKQLQRTS HNPLHQLSTP
HALSQLQKLL EEQHKINQMN IQKKEQERQQ AEIQRILLQQ ANAAQINHSF GLERLTPEYD
DNHHSETISK ASSEDLKTEP DSTDFGLGTS DDQVRASMLH LLHPVFAPAF GMLDAENIFG
AASKPTTPAS KRRNTDSNGA PSKKHRWLPV NELEESRSSR GKNCGRVHCK ATYKCALCGK
PTTLNSTGSR WNLLRHVIMI HSDCKPYKCW DCDFTGIKSN VISHARQCRH NAEDAHDITT
DEMRAEWNLR LHECFPDYVR AKERGWQPEE VTVKKEEVEE SPTLVKQELT LVKQEPTFAE
QLEPMAQPLV
