LIN28_CAEEL
ID LIN28_CAEEL Reviewed; 227 AA.
AC P92186; Q9U3K6;
DT 17-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1997, sequence version 1.
DT 03-AUG-2022, entry version 166.
DE RecName: Full=Protein lin-28 {ECO:0000305};
DE AltName: Full=Abnormal cell lineage protein 28 {ECO:0000312|WormBase:F02E9.2a};
GN Name=lin-28 {ECO:0000312|WormBase:F02E9.2a};
GN ORFNames=F02E9.2 {ECO:0000312|WormBase:F02E9.2a};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM A), FUNCTION, SUBCELLULAR
RP LOCATION, DEVELOPMENTAL STAGE, INDUCTION, AND MUTAGENESIS OF GLY-55;
RP MET-87; ARG-91; GLY-128 AND PRO-133.
RC STRAIN=Bristol N2;
RX PubMed=9054503; DOI=10.1016/s0092-8674(00)81906-6;
RA Moss E.G., Lee R.C., Ambros V.;
RT "The cold shock domain protein LIN-28 controls developmental timing in C.
RT elegans and is regulated by the lin-4 RNA.";
RL Cell 88:637-646(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [3]
RP FUNCTION.
RX PubMed=6494891; DOI=10.1126/science.6494891;
RA Ambros V., Horvitz H.R.;
RT "Heterochronic mutants of the nematode Caenorhabditis elegans.";
RL Science 226:409-416(1984).
RN [4]
RP FUNCTION.
RX PubMed=2702689; DOI=10.1016/0092-8674(89)90171-2;
RA Ambros V.;
RT "A hierarchy of regulatory genes controls a larva-to-adult developmental
RT switch in C. elegans.";
RL Cell 57:49-57(1989).
RN [5]
RP FUNCTION.
RX PubMed=2628162; DOI=10.1101/gad.3.12b.2039;
RA Liu Z.C., Ambros V.;
RT "Heterochronic genes control the stage-specific initiation and expression
RT of the dauer larva developmental program in Caenorhabditis elegans.";
RL Genes Dev. 3:2039-2049(1989).
RN [6]
RP FUNCTION.
RX PubMed=1916265; DOI=10.1101/gad.5.10.1825;
RA Arasu P., Wightman B., Ruvkun G.;
RT "Temporal regulation of lin-14 by the antagonistic action of two other
RT heterochronic genes, lin-4 and lin-28.";
RL Genes Dev. 5:1825-1833(1991).
RN [7]
RP FUNCTION.
RX PubMed=7671811; DOI=10.1242/dev.121.8.2471;
RA Liu Z.C., Kirch S., Ambros V.;
RT "The Caenorhabditis elegans heterochronic gene pathway controls stage-
RT specific transcription of collagen genes.";
RL Development 121:2471-2478(1995).
RN [8]
RP FUNCTION.
RX PubMed=8625405; DOI=10.1016/s0092-8674(00)81045-4;
RA Euling S., Ambros V.;
RT "Heterochronic genes control cell cycle progress and developmental
RT competence of C. elegans vulva precursor cells.";
RL Cell 84:667-676(1996).
RN [9]
RP FUNCTION.
RX PubMed=8756295; DOI=10.1242/dev.122.8.2507;
RA Euling S., Ambros V.;
RT "Reversal of cell fate determination in Caenorhabditis elegans vulval
RT development.";
RL Development 122:2507-2515(1996).
RN [10]
RP FUNCTION.
RX PubMed=8756296; DOI=10.1242/dev.122.8.2517;
RA Bettinger J.C., Lee K., Rougvie A.E.;
RT "Stage-specific accumulation of the terminal differentiation factor LIN-29
RT during Caenorhabditis elegans development.";
RL Development 122:2517-2527(1996).
RN [11]
RP FUNCTION.
RX PubMed=9477318; DOI=10.1242/dev.125.7.1191;
RA Antebi A., Culotti J.G., Hedgecock E.M.;
RT "daf-12 regulates developmental age and the dauer alternative in
RT Caenorhabditis elegans.";
RL Development 125:1191-1205(1998).
RN [12]
RP FUNCTION.
RX PubMed=9649524; DOI=10.1093/genetics/149.3.1335;
RA Abrahante J.E., Miller E.A., Rougvie A.E.;
RT "Identification of heterochronic mutants in Caenorhabditis elegans.
RT Temporal misexpression of a collagen::green fluorescent protein fusion
RT gene.";
RL Genetics 149:1335-1351(1998).
RN [13]
RP FUNCTION.
RX PubMed=10706289; DOI=10.1038/35002607;
RA Reinhart B.J., Slack F.J., Basson M., Pasquinelli A.E., Bettinger J.C.,
RA Rougvie A.E., Horvitz H.R., Ruvkun G.;
RT "The 21-nucleotide let-7 RNA regulates developmental timing in
RT Caenorhabditis elegans.";
RL Nature 403:901-906(2000).
RN [14]
RP DEVELOPMENTAL STAGE, AND INDUCTION.
RX PubMed=11884032; DOI=10.1006/dbio.2001.0563;
RA Seggerson K., Tang L., Moss E.G.;
RT "Two genetic circuits repress the Caenorhabditis elegans heterochronic gene
RT lin-28 after translation initiation.";
RL Dev. Biol. 243:215-225(2002).
RN [15]
RP FUNCTION.
RX PubMed=12871707; DOI=10.1016/s0012-1606(03)00202-1;
RA Johnson S.M., Lin S.-Y., Slack F.J.;
RT "The time of appearance of the C. elegans let-7 microRNA is
RT transcriptionally controlled utilizing a temporal regulatory element in its
RT promoter.";
RL Dev. Biol. 259:364-379(2003).
RN [16]
RP INDUCTION.
RX PubMed=15380030; DOI=10.1186/1471-213x-4-11;
RA Liu T., Zimmerman K.K., Patterson G.I.;
RT "Regulation of signaling genes by TGFbeta during entry into dauer diapause
RT in C. elegans.";
RL BMC Dev. Biol. 4:11-11(2004).
RN [17]
RP FUNCTION.
RX PubMed=15073154; DOI=10.1242/dev.01098;
RA Pepper A.-S., McCane J.E., Kemper K., Yeung D.A., Lee R.C., Ambros V.,
RA Moss E.G.;
RT "The C. elegans heterochronic gene lin-46 affects developmental timing at
RT two larval stages and encodes a relative of the scaffolding protein
RT gephyrin.";
RL Development 131:2049-2059(2004).
RN [18]
RP DEVELOPMENTAL STAGE, AND INDUCTION.
RX PubMed=16122423; DOI=10.1016/j.cell.2005.07.031;
RA Bagga S., Bracht J., Hunter S., Massirer K., Holtz J., Eachus R.,
RA Pasquinelli A.E.;
RT "Regulation by let-7 and lin-4 miRNAs results in target mRNA degradation.";
RL Cell 122:553-563(2005).
RN [19]
RP FUNCTION, AND DEVELOPMENTAL STAGE.
RX PubMed=16139228; DOI=10.1016/j.devcel.2005.07.009;
RA Abbott A.L., Alvarez-Saavedra E., Miska E.A., Lau N.C., Bartel D.P.,
RA Horvitz H.R., Ambros V.;
RT "The let-7 MicroRNA family members mir-48, mir-84, and mir-241 function
RT together to regulate developmental timing in Caenorhabditis elegans.";
RL Dev. Cell 9:403-414(2005).
RN [20]
RP FUNCTION, AND DEVELOPMENTAL STAGE.
RX PubMed=21471153; DOI=10.1242/dev.057109;
RA Huang X., Zhang H., Zhang H.;
RT "The zinc-finger protein SEA-2 regulates larval developmental timing and
RT adult lifespan in C. elegans.";
RL Development 138:2059-2068(2011).
RN [21]
RP PROTEOLYTIC CLEAVAGE, DISRUPTION PHENOTYPE, AND MUTAGENESIS OF ASP-11;
RP GLU-23; ASP-24 AND ASP-31.
RX PubMed=25432023; DOI=10.7554/elife.04265;
RA Weaver B.P., Zabinsky R., Weaver Y.M., Lee E.S., Xue D., Han M.;
RT "CED-3 caspase acts with miRNAs to regulate non-apoptotic gene expression
RT dynamics for robust development in C. elegans.";
RL Elife 3:E04265-E04265(2014).
RN [22]
RP FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=26811380; DOI=10.1242/dev.132738;
RA Herrera R.A., Kiontke K., Fitch D.H.;
RT "Makorin ortholog LEP-2 regulates LIN-28 stability to promote the juvenile-
RT to-adult transition in Caenorhabditis elegans.";
RL Development 143:799-809(2016).
RN [23]
RP FUNCTION, PROTEOLYTIC CLEAVAGE, AND MUTAGENESIS OF 2-SER--ASN-32; ASP-31
RP AND ASN-32.
RX PubMed=28602583; DOI=10.1016/j.devcel.2017.05.013;
RA Weaver B.P., Weaver Y.M., Mitani S., Han M.;
RT "Coupled Caspase and N-End Rule Ligase Activities Allow Recognition and
RT Degradation of Pluripotency Factor LIN-28 during Non-Apoptotic
RT Development.";
RL Dev. Cell 41:665-673(2017).
RN [24]
RP FUNCTION, AND DEVELOPMENTAL STAGE.
RX PubMed=31264582; DOI=10.7554/elife.43660;
RA Lawson H., Vuong E., Miller R.M., Kiontke K., Fitch D.H., Portman D.S.;
RT "The Makorin lep-2 and the lncRNA lep-5 regulate lin-28 to schedule sexual
RT maturation of the C. elegans nervous system.";
RL Elife 8:0-0(2019).
RN [25]
RP FUNCTION, IDENTIFICATION IN COMPLEX WITH LEP-2, DEVELOPMENTAL STAGE, AND
RP DISRUPTION PHENOTYPE.
RX PubMed=30956008; DOI=10.1016/j.devcel.2019.03.003;
RA Kiontke K.C., Herrera R.A., Vuong E., Luo J., Schwarz E.M., Fitch D.H.A.,
RA Portman D.S.;
RT "The Long Non-Coding RNA lep-5 Promotes the Juvenile-to-Adult Transition by
RT Destabilizing LIN-28.";
RL Dev. Cell 49:542-555(2019).
CC -!- FUNCTION: Heterochronic protein which controls the choice of stage
CC specific cell fates (PubMed:9054503, PubMed:6494891, PubMed:2702689,
CC PubMed:2628162, PubMed:1916265, PubMed:7671811, PubMed:8625405,
CC PubMed:8756295, PubMed:8756296, PubMed:9477318, PubMed:9649524,
CC PubMed:10706289, PubMed:12871707, PubMed:15073154, PubMed:16139228).
CC Regulates the timing of the second larval stage events (L2 events) in
CC the hypodermis (PubMed:2702689, PubMed:2628162). May negatively
CC regulate the larval to adult transition via the suppression of the
CC microRNA (miRNA) let-7 during L3 (PubMed:10706289, PubMed:12871707,
CC PubMed:16139228). Through this regulatory role, controls the timing of
CC the sexual maturation of the nervous system (PubMed:31264582). Also has
CC a role in the fox-1-sex-1-mediated determination of sexual fate
CC (PubMed:21471153). Plays a role in governing the developmental timing
CC of male tail tip morphogenesis (PubMed:26811380, PubMed:30956008).
CC Plays a role in controlling the seam cell number during larval stages
CC (PubMed:21471153, PubMed:28602583). Plays a role in vulval development
CC (PubMed:28602583). {ECO:0000269|PubMed:10706289,
CC ECO:0000269|PubMed:12871707, ECO:0000269|PubMed:15073154,
CC ECO:0000269|PubMed:16139228, ECO:0000269|PubMed:1916265,
CC ECO:0000269|PubMed:21471153, ECO:0000269|PubMed:2628162,
CC ECO:0000269|PubMed:26811380, ECO:0000269|PubMed:2702689,
CC ECO:0000269|PubMed:28602583, ECO:0000269|PubMed:30956008,
CC ECO:0000269|PubMed:31264582, ECO:0000269|PubMed:6494891,
CC ECO:0000269|PubMed:7671811, ECO:0000269|PubMed:8625405,
CC ECO:0000269|PubMed:8756295, ECO:0000269|PubMed:8756296,
CC ECO:0000269|PubMed:9054503, ECO:0000269|PubMed:9477318,
CC ECO:0000269|PubMed:9649524}.
CC -!- SUBUNIT: Component of a complex at least containing lep-2, lin-28 and
CC the long non-coding RNA lep-5, which mediates the degradation of lin-
CC 28. {ECO:0000269|PubMed:30956008}.
CC -!- INTERACTION:
CC P92186-2; Q09408: pup-2; NbExp=2; IntAct=EBI-15801711, EBI-15801698;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:26811380,
CC ECO:0000269|PubMed:9054503}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=a {ECO:0000312|WormBase:F02E9.2a};
CC IsoId=P92186-1; Sequence=Displayed;
CC Name=b {ECO:0000312|WormBase:F02E9.2b};
CC IsoId=P92186-2; Sequence=VSP_021122, VSP_021123;
CC -!- DEVELOPMENTAL STAGE: Expressed at the first larval stage (L1) in
CC several cell types including hypodermis, muscle, neurons and seam cells
CC (PubMed:9054503, PubMed:11884032, PubMed:16122423, PubMed:16139228,
CC PubMed:26811380, PubMed:31264582, PubMed:30956008, PubMed:21471153).
CC Also expressed in the tail tip and pharynx at the L1 stage
CC (PubMed:26811380). Down-regulated at L2, and absent from L3 and L4
CC (PubMed:9054503, PubMed:11884032, PubMed:16122423, PubMed:16139228,
CC PubMed:26811380, PubMed:31264582, PubMed:30956008, PubMed:21471153).
CC {ECO:0000269|PubMed:11884032, ECO:0000269|PubMed:16122423,
CC ECO:0000269|PubMed:16139228, ECO:0000269|PubMed:21471153,
CC ECO:0000269|PubMed:26811380, ECO:0000269|PubMed:30956008,
CC ECO:0000269|PubMed:31264582, ECO:0000269|PubMed:9054503}.
CC -!- INDUCTION: Negatively regulated by the miRNA lin-4 which causes
CC degradation of the mRNA encoding this protein. This requires a lin-4
CC complementary element (LCE) in the 3'-UTR of the mRNA encoding this
CC protein. Also negatively regulated independent of lin-4 and this is
CC counteracted by the action of lin-14. Positively regulated by TGF-beta
CC signaling. {ECO:0000269|PubMed:11884032, ECO:0000269|PubMed:15380030,
CC ECO:0000269|PubMed:16122423, ECO:0000269|PubMed:9054503}.
CC -!- PTM: Cleavage by caspase ced-3 during larval development probably
CC induces lin-28 degradation. {ECO:0000269|PubMed:25432023,
CC ECO:0000269|PubMed:28602583}.
CC -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown in a ced-3 and ain-1
CC double mutant background reduces the percentage of animals with
CC developmental defects including impaired egg-laying and production of
CC ectopic seam cells. RNAi-mediated knockdown results in the precocious
CC onset of tail tip retraction at the L3 larval stage resulting in over-
CC retracted and shortened adult male tails (also known as the Ore
CC phenotype) (PubMed:26811380, PubMed:30956008). RNAi-mediated knockdown
CC suppresses the male tail tip morphogenesis defects of mutants for the
CC long non-coding RNA lep-5 (PubMed:30956008).
CC {ECO:0000269|PubMed:25432023, ECO:0000269|PubMed:26811380,
CC ECO:0000269|PubMed:30956008}.
CC -!- SIMILARITY: Belongs to the lin-28 family. {ECO:0000305}.
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DR EMBL; U75912; AAC47476.1; -; mRNA.
DR EMBL; U75915; AAB49759.1; -; Genomic_DNA.
DR EMBL; BX284601; CAB61008.1; -; Genomic_DNA.
DR EMBL; BX284601; CAB61009.1; -; Genomic_DNA.
DR RefSeq; NP_001021085.1; NM_001025914.4. [P92186-1]
DR RefSeq; NP_492281.2; NM_059880.5. [P92186-2]
DR AlphaFoldDB; P92186; -.
DR SMR; P92186; -.
DR BioGRID; 38059; 6.
DR ComplexPortal; CPX-5081; lep-2-lep-5-lin-28 ubiquitin ligase complex.
DR DIP; DIP-40151N; -.
DR IntAct; P92186; 4.
DR STRING; 6239.F02E9.2a; -.
DR iPTMnet; P92186; -.
DR EPD; P92186; -.
DR PaxDb; P92186; -.
DR PeptideAtlas; P92186; -.
DR EnsemblMetazoa; F02E9.2a.1; F02E9.2a.1; WBGene00003014. [P92186-1]
DR EnsemblMetazoa; F02E9.2b.1; F02E9.2b.1; WBGene00003014. [P92186-2]
DR GeneID; 172626; -.
DR KEGG; cel:CELE_F02E9.2; -.
DR UCSC; F02E9.2a; c. elegans. [P92186-1]
DR CTD; 38639; -.
DR WormBase; F02E9.2a; CE24879; WBGene00003014; lin-28. [P92186-1]
DR WormBase; F02E9.2b; CE24880; WBGene00003014; lin-28. [P92186-2]
DR eggNOG; KOG3070; Eukaryota.
DR InParanoid; P92186; -.
DR OMA; EPPQSHC; -.
DR OrthoDB; 1604809at2759; -.
DR PhylomeDB; P92186; -.
DR PRO; PR:P92186; -.
DR Proteomes; UP000001940; Chromosome I.
DR Bgee; WBGene00003014; Expressed in pharyngeal muscle cell (C elegans) and 4 other tissues.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0005634; C:nucleus; IDA:WormBase.
DR GO; GO:0000151; C:ubiquitin ligase complex; IC:ComplexPortal.
DR GO; GO:0019899; F:enzyme binding; IPI:WormBase.
DR GO; GO:0003730; F:mRNA 3'-UTR binding; IDA:WormBase.
DR GO; GO:0003729; F:mRNA binding; IDA:WormBase.
DR GO; GO:1990715; F:mRNA CDS binding; IDA:WormBase.
DR GO; GO:0070883; F:pre-miRNA binding; IPI:WormBase.
DR GO; GO:0070878; F:primary miRNA binding; IDA:WormBase.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0001708; P:cell fate specification; IMP:UniProtKB.
DR GO; GO:0007549; P:dosage compensation; IGI:WormBase.
DR GO; GO:0010629; P:negative regulation of gene expression; IMP:UniProtKB.
DR GO; GO:2000635; P:negative regulation of primary miRNA processing; IMP:WormBase.
DR GO; GO:0002119; P:nematode larval development; IC:ComplexPortal.
DR GO; GO:0042551; P:neuron maturation; IC:ComplexPortal.
DR GO; GO:0031054; P:pre-miRNA processing; IDA:WormBase.
DR GO; GO:0042659; P:regulation of cell fate specification; IGI:UniProtKB.
DR GO; GO:0040034; P:regulation of development, heterochronic; IMP:WormBase.
DR GO; GO:0006511; P:ubiquitin-dependent protein catabolic process; IC:ComplexPortal.
DR CDD; cd04458; CSP_CDS; 1.
DR Gene3D; 2.40.50.140; -; 1.
DR InterPro; IPR011129; CSD.
DR InterPro; IPR002059; CSP_DNA-bd.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR001878; Znf_CCHC.
DR InterPro; IPR036875; Znf_CCHC_sf.
DR Pfam; PF00313; CSD; 1.
DR Pfam; PF00098; zf-CCHC; 1.
DR PRINTS; PR00050; COLDSHOCK.
DR SMART; SM00357; CSP; 1.
DR SMART; SM00343; ZnF_C2HC; 2.
DR SUPFAM; SSF50249; SSF50249; 1.
DR SUPFAM; SSF57756; SSF57756; 1.
DR PROSITE; PS51857; CSD_2; 1.
DR PROSITE; PS50158; ZF_CCHC; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cytoplasm; Developmental protein; Metal-binding;
KW Reference proteome; Repeat; Zinc; Zinc-finger.
FT CHAIN 1..227
FT /note="Protein lin-28"
FT /id="PRO_0000253799"
FT DOMAIN 52..120
FT /note="CSD"
FT ZN_FING 143..160
FT /note="CCHC-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00047"
FT ZN_FING 166..183
FT /note="CCHC-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00047"
FT REGION 1..27
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 181..227
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 144
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 147
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 153
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 158
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 168
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 171
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 176
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 181
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT SITE 31..32
FT /note="Cleavage; by ced-3"
FT /evidence="ECO:0000269|PubMed:25432023,
FT ECO:0000269|PubMed:28602583"
FT VAR_SEQ 1..16
FT /note="MSTVVSEGRNDGNNRY -> MIEAALENPVPIKSQL (in isoform b)"
FT /evidence="ECO:0000305"
FT /id="VSP_021122"
FT VAR_SEQ 17..47
FT /note="Missing (in isoform b)"
FT /evidence="ECO:0000305"
FT /id="VSP_021123"
FT MUTAGEN 2..32
FT /note="Missing: Constitutively active and results in
FT reduced lin-28 protein levels. Rescues the seam cell number
FT and vulval developmental defects of the lin-28 n719 loss of
FT function mutant."
FT /evidence="ECO:0000269|PubMed:28602583"
FT MUTAGEN 11
FT /note="D->A: Reduced ced-3-mediated cleavage in vitro."
FT /evidence="ECO:0000269|PubMed:25432023"
FT MUTAGEN 23
FT /note="E->A: Reduced ced-3-mediated cleavage in vitro."
FT /evidence="ECO:0000269|PubMed:25432023"
FT MUTAGEN 24
FT /note="D->A: Reduced ced-3-mediated cleavage in vitro."
FT /evidence="ECO:0000269|PubMed:25432023"
FT MUTAGEN 31
FT /note="D->A: Loss of ced-3-mediated cleavage in vitro.
FT Partially prevents reduction of lin-28 protein levels in
FT vivo. About 20 percent of mutants fail to reach adulthood.
FT Defects in alae morphology."
FT /evidence="ECO:0000269|PubMed:25432023,
FT ECO:0000269|PubMed:28602583"
FT MUTAGEN 32
FT /note="N->M: Loss of ced-3-mediated cleavage in vitro.
FT Prevents reduction of lin-28 protein levels in vitro."
FT /evidence="ECO:0000269|PubMed:28602583"
FT MUTAGEN 55
FT /note="G->S: In n1119; loss of function."
FT /evidence="ECO:0000269|PubMed:9054503"
FT MUTAGEN 87
FT /note="M->I: In ga73; when associated with Q-91."
FT /evidence="ECO:0000269|PubMed:9054503"
FT MUTAGEN 91
FT /note="R->Q: In ga73; when associated with I-87."
FT /evidence="ECO:0000269|PubMed:9054503"
FT MUTAGEN 128
FT /note="G->E: In ma157."
FT /evidence="ECO:0000269|PubMed:9054503"
FT MUTAGEN 128
FT /note="G->R: In sy283."
FT /evidence="ECO:0000269|PubMed:9054503"
FT MUTAGEN 133
FT /note="P->S: In ve9."
FT /evidence="ECO:0000269|PubMed:9054503"
SQ SEQUENCE 227 AA; 25465 MW; 5E4AE901AD8BAEBC CRC64;
MSTVVSEGRN DGNNRYSPQD EVEDRLPDVV DNRLTENMRV PSFERLPSPT PRYFGSCKWF
NVSKGYGFVI DDITGEDLFV HQSNLNMQGF RSLDEGERVS YYIQERSNGK GREAYAVSGE
VEGQGLKGSR IHPLGRKKAV SLRCFRCGKF ATHKAKSCPN VKTDAKVCYT CGSEEHVSSI
CPERRRKHRP EQVAAEEAEA ARMAAEKSSP TTSDDDIREK NSNSSDE
