LIN32_CAEEL
ID LIN32_CAEEL Reviewed; 142 AA.
AC Q10574;
DT 02-MAY-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2004, sequence version 2.
DT 03-AUG-2022, entry version 135.
DE RecName: Full=Protein lin-32;
DE AltName: Full=Abnormal cell lineage protein 32;
GN Name=lin-32 {ECO:0000312|WormBase:T14F9.5};
GN Synonyms=hlh-7 {ECO:0000312|WormBase:T14F9.5};
GN ORFNames=T14F9.5 {ECO:0000312|WormBase:T14F9.5};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION, AND
RP MUTAGENESIS OF GLU-81 AND LEU-95.
RC STRAIN=Bristol N2;
RX PubMed=7800042; DOI=10.1038/373074a0;
RA Zhao C., Emmons S.W.;
RT "A transcription factor controlling development of peripheral sense organs
RT in C. elegans.";
RL Nature 373:74-78(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [3]
RP FUNCTION, INTERACTION WITH HLH-2, DEVELOPMENTAL STAGE, AND MUTAGENESIS OF
RP GLU-81 AND LEU-95.
RX PubMed=11076762; DOI=10.1242/dev.127.24.5415;
RA Portman D.S., Emmons S.W.;
RT "The basic helix-loop-helix transcription factors LIN-32 and HLH-2 function
RT together in multiple steps of a C. elegans neuronal sublineage.";
RL Development 127:5415-5426(2000).
RN [4]
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND DEVELOPMENTAL
RP STAGE.
RX PubMed=31386623; DOI=10.7554/elife.46703;
RA Lee J., Taylor C.A., Barnes K.M., Shen A., Stewart E.V., Chen A.,
RA Xiang Y.K., Bao Z., Shen K.;
RT "A Myt1 family transcription factor defines neuronal fate by repressing
RT non-neuronal genes.";
RL Elife 8:0-0(2019).
RN [5]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=32665354; DOI=10.1534/g3.120.401547;
RA Zhang A., Noma K., Yan D.;
RT "Regulation of Gliogenesis by lin-32/Atoh1 in Caenorhabditis elegans.";
RL G3 (Bethesda) 10:3271-3278(2020).
CC -!- FUNCTION: Probable transcription factor which binds the E box motif 5'-
CC CA[TC][AG]TG-3' (PubMed:11076762). Essential for the specification of
CC the neuroblast cell fate in the development of peripheral sense organs
CC (PubMed:7800042). Its role in the generation of sensory neurons may be
CC through positively regulating the expression of the zinc finger protein
CC ztf-11 during postdeirid neurogenesis (PubMed:31386623). Required for
CC specification of cell fate, acting in concert with lin-32, in the
CC development of the male-specific genital sensilla (simple sense organs)
CC known as rays (PubMed:11076762). Involved in regulating glial
CC specification, perhaps by suppressing a glial fate in different
CC lineages during early embryogenesis (PubMed:32665354).
CC {ECO:0000269|PubMed:11076762, ECO:0000269|PubMed:31386623,
CC ECO:0000269|PubMed:32665354, ECO:0000269|PubMed:7800042}.
CC -!- SUBUNIT: Forms a heterodimer with hlh-2. {ECO:0000269|PubMed:11076762}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:31386623,
CC ECO:0000269|PubMed:7800042}.
CC -!- TISSUE SPECIFICITY: Expressed in PVD motor neurons.
CC {ECO:0000269|PubMed:31386623}.
CC -!- DEVELOPMENTAL STAGE: Expressed in V5 postdeirid lineage cells in L2
CC larvae (PubMed:31386623). Expressed in the nine ray lineage Rn.a
CC neuroblasts and their progeny, Rn.aa and Rn.ap (PubMed:11076762).
CC {ECO:0000269|PubMed:11076762, ECO:0000269|PubMed:31386623}.
CC -!- DISRUPTION PHENOTYPE: Knockout results in ectopic AMsh glial cells and
CC also ectopic amphid socket (AMso) cells, another type of glia, from a
CC different cell lineage (PubMed:32665354). Some animals are missing
CC dorsal, but not ventral, CEPsh glial cells (PubMed:32665354). Mean
CC number of ectopic AMsh glial cells increases on cnd-1 or ngn-1 mutant
CC backgrounds (PubMed:32665354). {ECO:0000269|PubMed:32665354}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAA67360.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; U15418; AAA67360.1; ALT_SEQ; Genomic_DNA.
DR EMBL; BX284606; CCD68944.1; -; Genomic_DNA.
DR PIR; T29378; T29378.
DR RefSeq; NP_508410.2; NM_076009.2.
DR AlphaFoldDB; Q10574; -.
DR SMR; Q10574; -.
DR BioGRID; 56200; 2.
DR IntAct; Q10574; 2.
DR STRING; 6239.T14F9.5; -.
DR PaxDb; Q10574; -.
DR EnsemblMetazoa; T14F9.5.1; T14F9.5.1; WBGene00003018.
DR GeneID; 191703; -.
DR KEGG; cel:CELE_T14F9.5; -.
DR UCSC; T14F9.5; c. elegans.
DR CTD; 191703; -.
DR WormBase; T14F9.5; CE33318; WBGene00003018; lin-32.
DR eggNOG; KOG4395; Eukaryota.
DR GeneTree; ENSGT00940000168200; -.
DR HOGENOM; CLU_1798134_0_0_1; -.
DR InParanoid; Q10574; -.
DR OMA; KKKCRRY; -.
DR OrthoDB; 1642317at2759; -.
DR PRO; PR:Q10574; -.
DR Proteomes; UP000001940; Chromosome X.
DR Bgee; WBGene00003018; Expressed in embryo and 3 other tissues.
DR GO; GO:0005634; C:nucleus; IDA:WormBase.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046982; F:protein heterodimerization activity; IPI:WormBase.
DR GO; GO:0042803; F:protein homodimerization activity; IPI:WormBase.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:WormBase.
DR GO; GO:0007568; P:aging; IMP:UniProtKB.
DR GO; GO:0009792; P:embryo development ending in birth or egg hatching; IMP:WormBase.
DR GO; GO:0003398; P:glial cell differentiation involved in amphid sensory organ development; IMP:UniProtKB.
DR GO; GO:0021780; P:glial cell fate specification; IMP:UniProtKB.
DR GO; GO:0051179; P:localization; IMP:WormBase.
DR GO; GO:0014014; P:negative regulation of gliogenesis; IMP:UniProtKB.
DR GO; GO:0045138; P:nematode male tail tip morphogenesis; IMP:WormBase.
DR GO; GO:0048666; P:neuron development; IMP:UniProtKB.
DR GO; GO:0030182; P:neuron differentiation; IBA:GO_Central.
DR GO; GO:0048665; P:neuron fate specification; IMP:UniProtKB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR Gene3D; 4.10.280.10; -; 1.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR036638; HLH_DNA-bd_sf.
DR Pfam; PF00010; HLH; 1.
DR SMART; SM00353; HLH; 1.
DR SUPFAM; SSF47459; SSF47459; 1.
DR PROSITE; PS50888; BHLH; 1.
PE 1: Evidence at protein level;
KW Developmental protein; Differentiation; DNA-binding; Neurogenesis; Nucleus;
KW Reference proteome.
FT CHAIN 1..142
FT /note="Protein lin-32"
FT /id="PRO_0000127261"
FT DOMAIN 72..124
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 30..66
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 30..47
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 81
FT /note="E->K: In u282; 99% loss of the male-specific genital
FT sensilla (simple sense organs) known as rays, and tail
FT touch insensitivity."
FT /evidence="ECO:0000269|PubMed:11076762,
FT ECO:0000269|PubMed:7800042"
FT MUTAGEN 95
FT /note="L->F: In e1926; aberrant development of all three
FT cell types forming the male-specific genital sensilla
FT (simple sense organs) known as rays. 81% ray loss. Ray loss
FT phenotype exacerbated on hlh-2 mutant background.
FT Drastically reduces binding to DNA as a heterodimer with
FT hlh-2."
FT /evidence="ECO:0000269|PubMed:11076762,
FT ECO:0000269|PubMed:7800042"
SQ SEQUENCE 142 AA; 16480 MW; DA20245666C65E8D CRC64;
MSWEQYQMYV PQCHPSFMYQ GSIQSTMTTP LQSPNFSLDS PNYPDSLSNG GGKDDKKKCR
RYKTPSPQLL RMRRSAANER ERRRMNTLNV AYDELREVLP EIDSGKKLSK FETLQMAQKY
IECLSQILKQ DSKNENLKSK SG
