ID   LIN50_BACLI             Reviewed;          15 AA.
AC   B3EWP7;
DT   09-DEC-2015, integrated into UniProtKB/Swiss-Prot.
DT   09-DEC-2015, sequence version 1.
DT   11-DEC-2019, entry version 3.
DE   RecName: Full=Bacteriocin licheniocin 50.2 {ECO:0000303|PubMed:24238327};
DE   Flags: Fragment;
OS   Bacillus licheniformis.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=1402 {ECO:0000303|PubMed:24238327};
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR
RP   LOCATION, AND MASS SPECTROMETRY.
RC   STRAIN=VPS50.2 {ECO:0000303|PubMed:24238327};
RX   PubMed=24238327; DOI=10.1111/jam.12393;
RA   Beric T., Stankovic S., Draganic V., Kojic M., Lozo J., Fira D.;
RT   "Novel antilisterial bacteriocin licheniocin 50.2 from Bacillus
RT   licheniformis VPS50.2 isolated from soil sample.";
RL   J. Appl. Microbiol. 116:502-510(2014).
CC   -!- FUNCTION: Bacteriocin active against species of Gram-positive bacterial
CC       genera Bacillus, Enterococcus, Lactobacillus, Lactococcus, Micrococcus,
CC       Staphylococcus and Streptococcus. Active against L.monocytogenes
CC       (MIC=0.53 mg/ml). Has no activity against various Gram-negative
CC       species. Has no activity against strains of fungi C.albicans and
CC       A.brasiliensis. {ECO:0000269|PubMed:24238327}.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       pH dependence:
CC         Stable from pH 2 to 12. {ECO:0000269|PubMed:24238327};
CC       Temperature dependence:
CC         Thermostable. Retains 100% activity after incubaction at 100 degrees
CC         Celsius for 30 minutes. Retains 70% activity after incubation at 121
CC         degrees Celsius at high pressure for 30 minutes.
CC         {ECO:0000269|PubMed:24238327};
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:24238327}.
CC   -!- MASS SPECTROMETRY: Mass=3253.209; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:24238327};
CC   -!- MISCELLANEOUS: Antibacterial activity is lost upon treatment with
CC       pronase E and partially with trypsin but not when treated with lysozyme
CC       or proteinase K. {ECO:0000269|PubMed:24238327}.
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DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0019835; P:cytolysis; IEA:UniProtKB-KW.
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Antibiotic; Antimicrobial; Bacteriocin; Direct protein sequencing;
KW   Secreted.
FT   PEPTIDE         1..>15
FT                   /note="Bacteriocin licheniocin 50.2"
FT                   /id="PRO_0000435029"
FT   NON_TER         15
FT                   /evidence="ECO:0000269|PubMed:24238327"
SQ   SEQUENCE   15 AA;  1803 MW;  F4AF028C60336401 CRC64;
     WEEYNIIXQL GNKGQ