LIN5_CAEEL
ID LIN5_CAEEL Reviewed; 821 AA.
AC P45970;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 30-MAY-2000, sequence version 2.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=Spindle apparatus protein lin-5;
DE AltName: Full=Abnormal cell lineage protein 5;
GN Name=lin-5; ORFNames=T09A5.10;
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND SUBCELLULAR LOCATION.
RC STRAIN=Bristol N2;
RX PubMed=10629219; DOI=10.1083/jcb.148.1.73;
RA Lorson M.A., Horvitz H.R., van den Heuvel S.;
RT "LIN-5 is a novel component of the spindle apparatus required for
RT chromosome segregation and cleavage plane specification in Caenorhabditis
RT elegans.";
RL J. Cell Biol. 148:73-86(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [3]
RP FUNCTION, AND INTERACTION WITH GPR-1.
RX PubMed=12730122; DOI=10.1101/gad.1081203;
RA Srinivasan D.G., Fisk R.M., Xu H., van den Heuvel S.;
RT "A complex of LIN-5 and GPR proteins regulates G protein signaling and
RT spindle function in C elegans.";
RL Genes Dev. 17:1225-1239(2003).
RN [4]
RP SUBCELLULAR LOCATION.
RX PubMed=18694560; DOI=10.1016/j.devcel.2008.06.002;
RA Panbianco C., Weinkove D., Zanin E., Jones D., Divecha N., Gotta M.,
RA Ahringer J.;
RT "A casein kinase 1 and PAR proteins regulate asymmetry of a PIP(2)
RT synthesis enzyme for asymmetric spindle positioning.";
RL Dev. Cell 15:198-208(2008).
CC -!- FUNCTION: Essential component of the spindle apparatus required for
CC spindle positioning and chromosome movement. Acts to recruit or anchor
CC gpr-1/gpr-2 complex to the spindle and cortex. Also involved, directly
CC or indirectly, in cytokinesis and in the coupling of DNA replication,
CC centrosome duplication and mitotic division.
CC {ECO:0000269|PubMed:10629219, ECO:0000269|PubMed:12730122}.
CC -!- SUBUNIT: Interacts with gpr-1; gpr-1 forms a complex with gpr-2 and
CC GDP-bound goa-1. {ECO:0000269|PubMed:12730122}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cell cortex
CC {ECO:0000269|PubMed:18694560}. Cytoplasm, cytoskeleton, spindle
CC {ECO:0000269|PubMed:10629219}. Chromosome, centromere, kinetochore
CC {ECO:0000269|PubMed:10629219}. Cytoplasm, cytoskeleton, microtubule
CC organizing center, centrosome {ECO:0000269|PubMed:10629219}.
CC Note=Located to the spindle apparatus in a cell cycle- and microtubule-
CC dependent manner. Located at the centrosomes throughout mitosis, at the
CC kinetochore microtubules in metaphase cells, and at the spindle during
CC meiosis (PubMed:10629219). Has enriched expression in the posterior
CC cortex of 1-cell embryos at anaphase, dependent on csnk-1 regulation of
CC pkk-1 (PubMed:18694560). {ECO:0000269|PubMed:10629219,
CC ECO:0000269|PubMed:18694560}.
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DR EMBL; AF240692; AAF61239.1; -; mRNA.
DR EMBL; Z36753; CAA85342.1; -; Genomic_DNA.
DR PIR; T24728; T24728.
DR RefSeq; NP_495654.1; NM_063253.5.
DR AlphaFoldDB; P45970; -.
DR SMR; P45970; -.
DR BioGRID; 39600; 21.
DR ComplexPortal; CPX-4027; gpr-1-gpr-2-lin-5 complex.
DR DIP; DIP-24397N; -.
DR IntAct; P45970; 2.
DR STRING; 6239.T09A5.10.2; -.
DR iPTMnet; P45970; -.
DR EPD; P45970; -.
DR PaxDb; P45970; -.
DR PeptideAtlas; P45970; -.
DR EnsemblMetazoa; T09A5.10.1; T09A5.10.1; WBGene00002994.
DR EnsemblMetazoa; T09A5.10.2; T09A5.10.2; WBGene00002994.
DR GeneID; 174267; -.
DR KEGG; cel:CELE_T09A5.10; -.
DR UCSC; T09A5.10.1; c. elegans.
DR CTD; 174267; -.
DR WormBase; T09A5.10; CE18951; WBGene00002994; lin-5.
DR eggNOG; ENOG502THVS; Eukaryota.
DR GeneTree; ENSGT00970000196590; -.
DR HOGENOM; CLU_022152_0_0_1; -.
DR InParanoid; P45970; -.
DR OMA; FMRSERE; -.
DR OrthoDB; 1049164at2759; -.
DR PhylomeDB; P45970; -.
DR PRO; PR:P45970; -.
DR Proteomes; UP000001940; Chromosome II.
DR Bgee; WBGene00002994; Expressed in germ line (C elegans) and 4 other tissues.
DR GO; GO:0005938; C:cell cortex; IDA:WormBase.
DR GO; GO:0000793; C:condensed chromosome; IDA:WormBase.
DR GO; GO:0005737; C:cytoplasm; IDA:WormBase.
DR GO; GO:0000776; C:kinetochore; IEA:UniProtKB-KW.
DR GO; GO:0005828; C:kinetochore microtubule; IDA:WormBase.
DR GO; GO:0005815; C:microtubule organizing center; IEA:UniProtKB-SubCell.
DR GO; GO:0072686; C:mitotic spindle; IDA:ComplexPortal.
DR GO; GO:0005819; C:spindle; IDA:WormBase.
DR GO; GO:0051233; C:spindle midzone; IDA:WormBase.
DR GO; GO:0000922; C:spindle pole; IDA:WormBase.
DR GO; GO:0070840; F:dynein complex binding; IDA:WormBase.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0051296; P:establishment of meiotic spindle orientation; IMP:WormBase.
DR GO; GO:0040001; P:establishment of mitotic spindle localization; IMP:WormBase.
DR GO; GO:0008406; P:gonad development; IMP:WormBase.
DR GO; GO:0040011; P:locomotion; IMP:WormBase.
DR GO; GO:0000022; P:mitotic spindle elongation; IMP:WormBase.
DR GO; GO:0007399; P:nervous system development; IMP:WormBase.
DR GO; GO:0007097; P:nuclear migration; IC:ComplexPortal.
DR GO; GO:0008277; P:regulation of G protein-coupled receptor signaling pathway; IC:ComplexPortal.
DR GO; GO:0032880; P:regulation of protein localization; IMP:WormBase.
DR GO; GO:0000003; P:reproduction; IMP:WormBase.
PE 1: Evidence at protein level;
KW Cell cycle; Cell division; Centromere; Chromosome; Coiled coil; Cytoplasm;
KW Cytoskeleton; Kinetochore; Mitosis; Reference proteome.
FT CHAIN 1..821
FT /note="Spindle apparatus protein lin-5"
FT /id="PRO_0000084432"
FT REGION 161..199
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 736..758
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 779..821
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 211..634
FT /evidence="ECO:0000255"
FT COMPBIAS 166..199
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 736..754
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 797..821
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 821 AA; 93440 MW; 1059BC1BE2CEFFC9 CRC64;
MSVSTSVVKS MLQLTNYFGS NFTPKTETIS SLAELWERHG SMIASMMSCD TREFGTADKF
YEVIFRKIAT TSLSFMTDSL EPKLAASGDS LEICKFYALF LEYMRKDHSG MLTRALEQAS
SENRVDTAVL LDMFLFFDNV YSESTADDWW ACLRRDDEDQ EASSGFRTPK RNNYSLTSLQ
TPTATARRLR TASSTARRSP IAEAVDSPTM KFMRSERELK QSKARIFGLE QMLGDLEDDK
TKLSAENRTL KLSNSDLKSD IAKLKGIAEE AQFRAEELSC DLEAKKDEVH NILQQLNESR
MTLRSEQRSL EEADIKKENL TAKLKTVTED NGKLMKQARE LRDLNDYEFA RFRQQEQELT
ETLRATQDQM ADLQEQLTGV EKIRASLKSE NESLSASVEE LSVASLRNKQ DADNSKTMLS
EELARFEETV DKLRQEKLEA LSMANSRIDA LRLEHSEREK MMKSTNARLQ ADLDEERNEK
KRLMNLWNEL NEKSLNVDKA VHKSNEEFQS MGSCLQNAKR QIEQLEVACK SKDDIIKFQE
EQNKRAANLI KNEKAIRDQA SAQFAEKLAI LKNSLAEKEN ETVTLKENFA SVVMKHKAEL
EEKELFLQSR VDLIQRLEHE VADLREKESA EIKKSTWMGE RIKIFQQAPM RGSRIRCGSP
DSLPDFLADN GPTITEEELR AQLATFPRKS IAPSVDDNEF DKGTPIGFKS NMYDGRESIC
SLDFFDRSSL QRSSMRSESI QLASPSSAGE FKQPFTPSGV TKERVGVLTA RNEKVKPHLK
CSYASEVGST NSPSADEENI KKSKKKNRRD SIFSAFSSKK Q
