LIN61_CAEEL
ID LIN61_CAEEL Reviewed; 612 AA.
AC B2D6M2; Q21769;
DT 16-MAY-2012, integrated into UniProtKB/Swiss-Prot.
DT 20-MAY-2008, sequence version 1.
DT 03-AUG-2022, entry version 84.
DE RecName: Full=Protein lin-61;
DE AltName: Full=Abnormal cell lineage protein 61;
GN Name=lin-61; ORFNames=R06C7.7;
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND ALTERNATIVE SPLICING.
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2]
RP FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE,
RP AND MUTAGENESIS OF PRO-253; PHE-368; GLY-371 AND SER-475.
RX PubMed=17409073; DOI=10.1534/genetics.106.069633;
RA Harrison M.M., Lu X., Horvitz H.R.;
RT "LIN-61, one of two Caenorhabditis elegans malignant-brain-tumor-repeat-
RT containing proteins, acts with the DRM and NuRD-like protein complexes in
RT vulval development but not in certain other biological processes.";
RL Genetics 176:255-271(2007).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY, FUNCTION, INTERACTION WITH HISTONE H3,
RP DISRUPTION PHENOTYPE, DOMAIN MBT, AND MUTAGENESIS OF ASP-549; PHE-573;
RP TRP-576 AND PHE-580.
RC STRAIN=Bristol N2;
RX PubMed=21437264; DOI=10.1371/journal.pgen.1002017;
RA Koester-Eiserfunke N., Fischle W.;
RT "H3K9me2/3 binding of the MBT domain protein LIN-61 is essential for
RT Caenorhabditis elegans vulva development.";
RL PLoS Genet. 7:E1002017-E1002017(2011).
CC -!- FUNCTION: Synthetic multivulva class B (synMuvB) protein required to
CC repress the induction of vulval development by Ras signaling. Unlike
CC other synMuv proteins it does not associate with the multiprotein DRM
CC complex and the NuRD-like complex. Interaction with methylated histone
CC H3 is essential for vulva development. It has a role in maintaining
CC genome stability. {ECO:0000269|PubMed:17409073,
CC ECO:0000269|PubMed:21437264}.
CC -!- SUBUNIT: Interacts preferentially with histone H3 that is dimethylated
CC or trimethylated at 'Lys-9'. {ECO:0000269|PubMed:21437264}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:17409073}. Chromosome
CC {ECO:0000269|PubMed:17409073}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=b;
CC IsoId=B2D6M2-1; Sequence=Displayed;
CC Name=a;
CC IsoId=B2D6M2-2; Sequence=VSP_043612;
CC -!- DEVELOPMENTAL STAGE: Expressed from the one-cell embryo to the adult
CC (at protein level). {ECO:0000269|PubMed:17409073}.
CC -!- DOMAIN: The MBT repeats specifically recognize and bind histone H3
CC di- and tri-methylated at 'Lys-9' (H3K9me2/3).
CC {ECO:0000269|PubMed:21437264}.
CC -!- DISRUPTION PHENOTYPE: Multivulva phenotype in combination with loss of
CC function of class A synMuv genes lin-8, lin-38, lin-56 and lin15A and
CC with the class B synMuv gene hpl-2. Reduction of brood size and defects
CC in the development of germ cells. Loss of function results in
CC suppression of mat-3(ku233) mutant vulval phenotype.
CC {ECO:0000269|PubMed:17409073, ECO:0000269|PubMed:21437264}.
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DR EMBL; Z71266; CAA95838.3; -; Genomic_DNA.
DR EMBL; Z71266; CAQ35053.1; -; Genomic_DNA.
DR PIR; T23964; T23964.
DR RefSeq; NP_001122501.1; NM_001129029.1. [B2D6M2-1]
DR RefSeq; NP_492050.3; NM_059649.4. [B2D6M2-2]
DR AlphaFoldDB; B2D6M2; -.
DR SMR; B2D6M2; -.
DR BioGRID; 37910; 3.
DR STRING; 6239.R06C7.7b; -.
DR EPD; B2D6M2; -.
DR PaxDb; B2D6M2; -.
DR PeptideAtlas; B2D6M2; -.
DR PRIDE; B2D6M2; -.
DR EnsemblMetazoa; R06C7.7a.1; R06C7.7a.1; WBGene00003041. [B2D6M2-2]
DR EnsemblMetazoa; R06C7.7b.1; R06C7.7b.1; WBGene00003041. [B2D6M2-1]
DR GeneID; 172467; -.
DR KEGG; cel:CELE_R06C7.7; -.
DR UCSC; R06C7.7a.1; c. elegans.
DR CTD; 172467; -.
DR WormBase; R06C7.7a; CE37542; WBGene00003041; lin-61. [B2D6M2-2]
DR WormBase; R06C7.7b; CE31573; WBGene00003041; lin-61. [B2D6M2-1]
DR eggNOG; KOG3766; Eukaryota.
DR GeneTree; ENSGT00940000169237; -.
DR InParanoid; B2D6M2; -.
DR OMA; ISECFAT; -.
DR OrthoDB; 1334498at2759; -.
DR PhylomeDB; B2D6M2; -.
DR PRO; PR:B2D6M2; -.
DR Proteomes; UP000001940; Chromosome I.
DR Bgee; WBGene00003041; Expressed in germ line (C elegans) and 4 other tissues.
DR GO; GO:0000785; C:chromatin; IDA:WormBase.
DR GO; GO:0005634; C:nucleus; IDA:WormBase.
DR GO; GO:0003682; F:chromatin binding; IBA:GO_Central.
DR GO; GO:0042393; F:histone binding; IBA:GO_Central.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IBA:GO_Central.
DR GO; GO:0040025; P:vulval development; IGI:WormBase.
DR InterPro; IPR004092; Mbt.
DR Pfam; PF02820; MBT; 3.
DR SMART; SM00561; MBT; 3.
DR PROSITE; PS51079; MBT; 4.
PE 1: Evidence at protein level;
KW Alternative splicing; Chromosome; Nucleus; Reference proteome; Repeat.
FT CHAIN 1..612
FT /note="Protein lin-61"
FT /id="PRO_0000417369"
FT REPEAT 143..249
FT /note="MBT 1"
FT REPEAT 263..380
FT /note="MBT 2"
FT REPEAT 381..501
FT /note="MBT 3"
FT REPEAT 508..607
FT /note="MBT 4"
FT VAR_SEQ 1..121
FT /note="Missing (in isoform a)"
FT /evidence="ECO:0000305"
FT /id="VSP_043612"
FT MUTAGEN 253
FT /note="P->S: In allele lin-61(n3624); does not affect
FT binding to histone H3 trimethylated at 'Lys-9'; multivulva
FT phenotype in combination with lin56(n2728)."
FT /evidence="ECO:0000269|PubMed:17409073"
FT MUTAGEN 368
FT /note="F->S: In allele lin-61(n3736); reduces binding to
FT histone H3 trimethylated at 'Lys-9'; multivulva phenotype
FT in combination with lin56(n2728)."
FT /evidence="ECO:0000269|PubMed:17409073"
FT MUTAGEN 371
FT /note="G->E: In allele lin-61(n3807); abolishes binding to
FT histone H3 trimethylated at 'Lys-9'; multivulva phenotype
FT in combination with lin56(n2728)."
FT /evidence="ECO:0000269|PubMed:17409073"
FT MUTAGEN 475
FT /note="S->N: In allele lin-61(n3447); reduces binding to
FT histone H3 trimethylated at 'Lys-9'; multivulva phenotype
FT in combination with lin56(n2728)."
FT /evidence="ECO:0000269|PubMed:17409073"
FT MUTAGEN 549
FT /note="D->A: Reduces binding to histone H3 trimethylated at
FT 'Lys-9'; in isoform a."
FT /evidence="ECO:0000269|PubMed:21437264"
FT MUTAGEN 573
FT /note="F->A: Abolishes binding to histone H3 trimethylated
FT at 'Lys-9'; when associated A-576 and A-580, in isoform a."
FT /evidence="ECO:0000269|PubMed:21437264"
FT MUTAGEN 576
FT /note="W->A: Abolishes binding to histone H3 trimethylated
FT at 'Lys-9'; in isoform a."
FT /evidence="ECO:0000269|PubMed:21437264"
FT MUTAGEN 580
FT /note="F->A: Abolishes binding to histone H3 trimethylated
FT at 'Lys-9'; in isoform a."
FT /evidence="ECO:0000269|PubMed:21437264"
SQ SEQUENCE 612 AA; 70910 MW; D0A1B0156C73C254 CRC64;
MLKLVILCFA LFYNTVSSTR FLFGVEVKCD FDEVFQLTVS HWEDDGNTFW DRDEDITGRM
TMFARKKIFF YQDGHHGFEF GKLEPYGWFL HNCTKNGNFR EYRHGLSSTS GSNGLEYIEY
TMSEFLKIVR ANKKSDRKLD KTYLWESYLH QFEKGKTSFI PVEAFNRNLT VNFNECVKEG
VIFETVVHDY DKNCDSIQVR WFARIEKVCG YRVLAQFIGA DTKFWLNILS DDMFGLANAA
MSDPNMDKIV YAPPLAINEE YQNDMVNYVN NCIDGEIVGQ TSLSPKFDEG KALLSKHRFK
VGQRLELLNY SNSTEIRVAR IQEICGRRMN VSITKKDFPE SLPDADDDRQ VFSSGSQYWI
DEGSFFIFPV GFAAVNGYQL NAKKEYIEHT NKIAQAIKNG ENPRYDSDDV TFDQLAKDPI
DPMIWRKVKV GQKFELIDPL AQQFNNLHVA SILKFCKTEG YLIVGMDGPD ALEDSFPIHI
NNTFMFPVGY AEKYNLELVP PDEFKGTFRW DEYLEKESAE TLPLDLFKPM PSQERLDKFK
VGLRLEAADM CENQFICPAT VKSVHGRLIN VNFDGWDEEF DELYDVDSHD ILPIGWCEAH
SYVLQPPKKY NY
