LIN8_CAEEL
ID LIN8_CAEEL Reviewed; 386 AA.
AC G5EDW7;
DT 30-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT 14-DEC-2011, sequence version 1.
DT 03-AUG-2022, entry version 68.
DE RecName: Full=Protein lin-8 {ECO:0000305};
DE AltName: Full=Abnormal cell lineage protein 8 {ECO:0000312|WormBase:B0454.1};
GN Name=lin-8 {ECO:0000312|WormBase:B0454.1};
GN ORFNames=B0454.1 {ECO:0000312|WormBase:B0454.1};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN [1] {ECO:0000312|EMBL:AAZ77787.1}
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH LIN-35, SUBCELLULAR
RP LOCATION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE, AND MUTAGENESIS OF
RP LEU-20; VAL-68; GLU-148; ARG-154 AND GLU-164.
RX PubMed=16020796; DOI=10.1534/genetics.104.034173;
RA Davison E.M., Harrison M.M., Walhout A.J., Vidal M., Horvitz H.R.;
RT "lin-8, which antagonizes Caenorhabditis elegans Ras-mediated vulval
RT induction, encodes a novel nuclear protein that interacts with the LIN-35
RT Rb protein.";
RL Genetics 171:1017-1031(2005).
RN [2] {ECO:0000312|Proteomes:UP000001940}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [3] {ECO:0000305}
RP FUNCTION, AND MUTAGENESIS OF LEU-20 AND ARG-154.
RX PubMed=16678779; DOI=10.1016/j.devcel.2006.04.001;
RA Cui M., Chen J., Myers T.R., Hwang B.J., Sternberg P.W., Greenwald I.,
RA Han M.;
RT "SynMuv genes redundantly inhibit lin-3/EGF expression to prevent
RT inappropriate vulval induction in C. elegans.";
RL Dev. Cell 10:667-672(2006).
CC -!- FUNCTION: Acts as a synthetic multivulva class A (synMuvA) protein and
CC redundantly inhibits lin-3/EGF expression to prevent inappropriate
CC vulva induction. {ECO:0000269|PubMed:16020796,
CC ECO:0000269|PubMed:16678779}.
CC -!- SUBUNIT: Interacts with lin-35 (via C-terminus).
CC {ECO:0000269|PubMed:16020796}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:16020796}.
CC -!- TISSUE SPECIFICITY: Widely expressed throughout development, with
CC particularly prominent expression in the germline and in neuronal
CC nuclei of the head (at protein level). {ECO:0000269|PubMed:16020796}.
CC -!- DISRUPTION PHENOTYPE: Temperature-sensitive multivulva phenotype in a
CC lin-15B n2245 or lin-52 n771 mutant background.
CC {ECO:0000269|PubMed:16020796}.
CC -!- SIMILARITY: Belongs to the lin-8 family. {ECO:0000305}.
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DR EMBL; DQ150101; AAZ77787.1; -; mRNA.
DR EMBL; BX284602; CCD61967.1; -; Genomic_DNA.
DR RefSeq; NP_494436.1; NM_062035.4.
DR AlphaFoldDB; G5EDW7; -.
DR IntAct; G5EDW7; 2.
DR STRING; 6239.B0454.1; -.
DR EPD; G5EDW7; -.
DR PaxDb; G5EDW7; -.
DR PeptideAtlas; G5EDW7; -.
DR EnsemblMetazoa; B0454.1.1; B0454.1.1; WBGene00002997.
DR GeneID; 173652; -.
DR KEGG; cel:CELE_B0454.1; -.
DR CTD; 173652; -.
DR WormBase; B0454.1; CE20455; WBGene00002997; lin-8.
DR eggNOG; ENOG502THHU; Eukaryota.
DR GeneTree; ENSGT00390000006206; -.
DR HOGENOM; CLU_055340_0_0_1; -.
DR InParanoid; G5EDW7; -.
DR OMA; REHTQHW; -.
DR OrthoDB; 1514757at2759; -.
DR PhylomeDB; G5EDW7; -.
DR PRO; PR:G5EDW7; -.
DR Proteomes; UP000001940; Chromosome II.
DR Bgee; WBGene00002997; Expressed in embryo and 4 other tissues.
DR GO; GO:0005634; C:nucleus; IDA:WormBase.
DR GO; GO:0040027; P:negative regulation of vulval development; IGI:WormBase.
DR InterPro; IPR005020; LIN-8.
DR PANTHER; PTHR32020; PTHR32020; 1.
DR Pfam; PF03353; Lin-8; 1.
PE 1: Evidence at protein level;
KW Developmental protein; Nucleus; Reference proteome.
FT CHAIN 1..386
FT /note="Protein lin-8"
FT /id="PRO_0000438591"
FT REGION 175..285
FT /note="Sufficient for interaction with lin-35"
FT /evidence="ECO:0000269|PubMed:16020796"
FT REGION 212..240
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 223..239
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 20
FT /note="L->P: In n111; multivulva phenotype in a lin-15B
FT n2245, lin-52 n771, lin-9 n112, lin-35 n745 or lin-37 n758
FT mutant background. Increased lin-3 mRNA levels in a lin-35
FT n745 mutant background as compared to wild-type."
FT /evidence="ECO:0000269|PubMed:16020796,
FT ECO:0000269|PubMed:16678779"
FT MUTAGEN 68
FT /note="V->M: In n2741; temperature-sensitive multivulva
FT phenotype in a lin-15B n2245 or lin-52 n771 mutant
FT background."
FT /evidence="ECO:0000269|PubMed:16020796"
FT MUTAGEN 148
FT /note="E->K: In n2376; temperature-sensitive multivulva
FT phenotype in a lin-15B n2245 or lin-52 n771 mutant
FT background."
FT /evidence="ECO:0000269|PubMed:16020796"
FT MUTAGEN 154
FT /note="R->C: In n2378; multivulva phenotype in a lin-15B
FT n2245, lin-52 n771 or lin-36 n766 mutant background."
FT /evidence="ECO:0000269|PubMed:16020796,
FT ECO:0000269|PubMed:16678779"
FT MUTAGEN 164
FT /note="E->K: In n2403; temperature-sensitive multivulva
FT phenotype in a lin-15B n2245 or lin-52 n771 mutant
FT background."
FT /evidence="ECO:0000269|PubMed:16020796"
SQ SEQUENCE 386 AA; 44180 MW; 753922A5B6246FD9 CRC64;
MSKIKTHSTG SKRTVPFYKL PPPVPLPPLP PPDPTRYFST EKYIALSKDE KFKFDDYDVN
DETLKKVVLN EIGKCPDIWS SRSQAAIMEH YPIVATETYR RTGLLLSIKS LKQIYKCGKD
NLRNRLRVAI VSKRLTPAQV EAYMWRWEFY GFIRYYRDYT QRWEADLLKD LDVVLGLEAR
RASKNMEKVD SGELMEPMEP MDSTMDEMCV EEEPYEETGS NWSDPAPEPS QSKSQSPEAK
YPQAYLLPEA DEVYNPDDFY QEEHESASNA MYRIAFSQQY GGGGSPAVQK PVTFSAQPAP
APVREAPSPV VENVSSSSFT PKPPAMINNF GEEMNQITYQ AIRIAREQPE RLKLLRKALF
DVVLAFDQKE YADVGDLYRD LAQKNS
