LINA1_SPHIB
ID LINA1_SPHIB Reviewed; 154 AA.
AC P59766; A0A1L5BUI1;
DT 03-JUL-2003, integrated into UniProtKB/Swiss-Prot.
DT 18-JUL-2018, sequence version 2.
DT 25-MAY-2022, entry version 52.
DE RecName: Full=Hexachlorocyclohexane dehydrochlorinase 1 {ECO:0000303|PubMed:12450824};
DE Short=HCH dehydrochlorinase 1 {ECO:0000303|PubMed:12450824};
DE EC=4.5.1.- {ECO:0000305|PubMed:12450824};
GN Name=linA1 {ECO:0000303|PubMed:12450824};
GN ORFNames=SIDU_17865 {ECO:0000312|EMBL:APL96514.1};
OS Sphingobium indicum (strain DSM 16412 / CCM 7286 / MTCC 6364 / B90A).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales;
OC Sphingomonadaceae; Sphingobium.
OX NCBI_TaxID=861109;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, AND
RP PATHWAY.
RC STRAIN=B90;
RX PubMed=12450824; DOI=10.1128/aem.68.12.6021-6028.2002;
RA Kumari R., Subudhi S., Suar M., Dhingra G., Raina V., Dogra C., Lal S.,
RA van der Meer J.R., Holliger C., Lal R.;
RT "Cloning and characterization of lin genes responsible for the degradation
RT of hexachlorocyclohexane isomers by Sphingomonas paucimobilis strain B90.";
RL Appl. Environ. Microbiol. 68:6021-6028(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 16412 / CCM 7286 / MTCC 6364 / B90A;
RX PubMed=22843598; DOI=10.1128/jb.00901-12;
RA Anand S., Sangwan N., Lata P., Kaur J., Dua A., Singh A.K., Verma M.,
RA Kaur J., Khurana J.P., Khurana P., Mathur S., Lal R.;
RT "Genome sequence of Sphingobium indicum B90A, a hexachlorocyclohexane-
RT degrading bacterium.";
RL J. Bacteriol. 194:4471-4472(2012).
CC -!- FUNCTION: Catalyzes the conversion of the important environmental
CC pollutant gamma-hexachlorocyclohexane (gamma-HCH or lindane) to
CC 1,3,4,6-tetrachloro-1,4-cyclohexadiene (1,4-TCDN) via gamma-
CC pentachlorocyclohexene (gamma-PCCH) (PubMed:12450824). Proceeds by two
CC successive 1,2-anti conformationally dependent dehydrochlorinations (By
CC similarity). Also shows activity with alpha- and delta-HCH, giving
CC alpha- and delta-PCCH respectively, but not with the beta isomer
CC (PubMed:12450824). {ECO:0000250|UniProtKB:P51697,
CC ECO:0000269|PubMed:12450824}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=gamma-hexachlorocyclohexane = (3R,4S,5S,6R)-
CC pentachlorocyclohexene + chloride + H(+); Xref=Rhea:RHEA:45480,
CC ChEBI:CHEBI:10576, ChEBI:CHEBI:15378, ChEBI:CHEBI:17996,
CC ChEBI:CHEBI:32888; Evidence={ECO:0000250|UniProtKB:P51697,
CC ECO:0000305|PubMed:12450824};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(3R,4S,5S,6R)-pentachlorocyclohexene = (3R,6R)-1,3,4,6-
CC tetrachlorocyclohexa-1,4-diene + chloride + H(+);
CC Xref=Rhea:RHEA:12152, ChEBI:CHEBI:10576, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:17996, ChEBI:CHEBI:18904;
CC Evidence={ECO:0000250|UniProtKB:P51697};
CC -!- PATHWAY: Xenobiotic degradation; hexachlorocyclohexane degradation.
CC {ECO:0000269|PubMed:12450824}.
CC -!- SUBUNIT: Homotrimer. {ECO:0000250|UniProtKB:P51697}.
CC -!- SUBCELLULAR LOCATION: Periplasm {ECO:0000250|UniProtKB:P51697}.
CC -!- MISCELLANEOUS: Is not N-terminally processed during export, so it may
CC be secreted into the periplasmic space via a hitherto unknown
CC mechanism. {ECO:0000250|UniProtKB:P51697}.
CC -!- MISCELLANEOUS: Gamma-hexachlorocyclohexane (lindane) is an
CC organochlorine insecticide which has been used worldwide since the
CC 1940s. Because of its toxicity and long persistence in soil, most
CC countries have prohibited the use of gamma-HCH. However, many
CC contaminated sites still remain throughout the world. {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the HCH dehydrochlorinase family. {ECO:0000305}.
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DR EMBL; AY150579; AAN64239.1; -; Genomic_DNA.
DR EMBL; CP013071; APL96514.1; -; Genomic_DNA.
DR RefSeq; WP_073507206.1; NZ_CP013071.1.
DR AlphaFoldDB; P59766; -.
DR SMR; P59766; -.
DR EnsemblBacteria; APL96514; APL96514; SIDU_17865.
DR KEGG; sinb:SIDU_17865; -.
DR OrthoDB; 1736098at2; -.
DR BRENDA; 4.5.1.B1; 8831.
DR UniPathway; UPA00690; -.
DR Proteomes; UP000004550; Plasmid pSRL1.
DR GO; GO:0042597; C:periplasmic space; IEA:UniProtKB-SubCell.
DR GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW.
DR GO; GO:0009636; P:response to toxic substance; IEA:UniProtKB-KW.
DR InterPro; IPR032710; NTF2-like_dom_sf.
DR InterPro; IPR037401; SnoaL-like.
DR Pfam; PF13577; SnoaL_4; 1.
DR SUPFAM; SSF54427; SSF54427; 1.
PE 1: Evidence at protein level;
KW Detoxification; Lyase; Periplasm.
FT CHAIN 1..154
FT /note="Hexachlorocyclohexane dehydrochlorinase 1"
FT /id="PRO_0000084434"
FT ACT_SITE 25
FT /evidence="ECO:0000250|UniProtKB:P51697"
FT ACT_SITE 73
FT /note="Proton acceptor"
FT /evidence="ECO:0000250|UniProtKB:P51697"
FT CONFLICT 68
FT /note="Y -> F (in Ref. 1; AAN64239)"
SQ SEQUENCE 154 AA; 17145 MW; 339D51B8A1C01481 CRC64;
MSDLDRLASR AAIQDLYSDQ LIGVDKRQEG RLASIWWDDA EWTIEGIGTY KGPEGALDLA
NNVLWPMYHE TIHYGTNLRL EFVSADKVNG IGDVLCLGNL VEGNQSILIA AVYTNEYERR
DGVWKLSKLN GCMNYFTPLA GIHFAPPGAL LQKS
