ID   LINC_SPHIB              Reviewed;         250 AA.
AC   A0A1L5BUG8; P50197;
DT   18-JUL-2018, integrated into UniProtKB/Swiss-Prot.
DT   15-MAR-2017, sequence version 1.
DT   03-AUG-2022, entry version 21.
DE   RecName: Full=2,5-dichloro-2,5-cyclohexadiene-1,4-diol dehydrogenase {ECO:0000250|UniProtKB:D4YYG1};
DE            Short=2,5-DDOL dehydrogenase {ECO:0000250|UniProtKB:D4YYG1};
DE            EC=1.1.1.- {ECO:0000250|UniProtKB:D4YYG1};
GN   Name=linB {ECO:0000303|PubMed:12450824};
GN   ORFNames=SIDU_17850 {ECO:0000312|EMBL:APL96511.1};
OS   Sphingobium indicum (strain DSM 16412 / CCM 7286 / MTCC 6364 / B90A).
OG   Plasmid pSRL1 {ECO:0000312|Proteomes:UP000004550}.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales;
OC   Sphingomonadaceae; Sphingobium.
OX   NCBI_TaxID=861109;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND PATHWAY.
RC   STRAIN=B90;
RX   PubMed=12450824; DOI=10.1128/aem.68.12.6021-6028.2002;
RA   Kumari R., Subudhi S., Suar M., Dhingra G., Raina V., Dogra C., Lal S.,
RA   van der Meer J.R., Holliger C., Lal R.;
RT   "Cloning and characterization of lin genes responsible for the degradation
RT   of hexachlorocyclohexane isomers by Sphingomonas paucimobilis strain B90.";
RL   Appl. Environ. Microbiol. 68:6021-6028(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 16412 / CCM 7286 / MTCC 6364 / B90A;
RX   PubMed=22843598; DOI=10.1128/jb.00901-12;
RA   Anand S., Sangwan N., Lata P., Kaur J., Dua A., Singh A.K., Verma M.,
RA   Kaur J., Khurana J.P., Khurana P., Mathur S., Lal R.;
RT   "Genome sequence of Sphingobium indicum B90A, a hexachlorocyclohexane-
RT   degrading bacterium.";
RL   J. Bacteriol. 194:4471-4472(2012).
CC   -!- FUNCTION: Catalyzes the dehydrogenation of 2,5-dichloro-2,5-
CC       cyclohexadiene-1,4-diol (2,5-DDOL) to 2,5-dichlorohydroquinone (2,5-
CC       DCHQ), a step in the degradation of gamma-hexachlorocyclohexane (gamma-
CC       HCH or lindane). {ECO:0000250|UniProtKB:D4YYG1,
CC       ECO:0000305|PubMed:12450824}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2,5-dichlorocyclohexa-2,5-dien-1,4-diol + NAD(+) = 2,5-
CC         dichlorohydroquinone + H(+) + NADH; Xref=Rhea:RHEA:15741,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:27545, ChEBI:CHEBI:28975,
CC         ChEBI:CHEBI:57540, ChEBI:CHEBI:57945;
CC         Evidence={ECO:0000250|UniProtKB:D4YYG1};
CC   -!- PATHWAY: Xenobiotic degradation; gamma-hexachlorocyclohexane
CC       degradation. {ECO:0000305|PubMed:12450824}.
CC   -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR)
CC       family. {ECO:0000305}.
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DR   EMBL; AY150582; AAN64242.1; -; Genomic_DNA.
DR   EMBL; CP013071; APL96511.1; -; Genomic_DNA.
DR   RefSeq; WP_015460611.1; NZ_CP013071.1.
DR   AlphaFoldDB; A0A1L5BUG8; -.
DR   SMR; A0A1L5BUG8; -.
DR   EnsemblBacteria; APL96511; APL96511; SIDU_17850.
DR   KEGG; sinb:SIDU_17850; -.
DR   OrthoDB; 1356861at2; -.
DR   UniPathway; UPA00689; -.
DR   Proteomes; UP000004550; Plasmid pSRL1.
DR   GO; GO:0018502; F:2,5-dichloro-2,5-cyclohexadiene-1,4-diol dehydrogenase activity; IEA:RHEA.
DR   GO; GO:0009636; P:response to toxic substance; IEA:UniProtKB-KW.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR020904; Sc_DH/Rdtase_CS.
DR   InterPro; IPR002347; SDR_fam.
DR   PRINTS; PR00081; GDHRDH.
DR   PRINTS; PR00080; SDRFAMILY.
DR   SUPFAM; SSF51735; SSF51735; 1.
DR   PROSITE; PS00061; ADH_SHORT; 1.
PE   3: Inferred from homology;
KW   Detoxification; NAD; Oxidoreductase; Plasmid.
FT   CHAIN           1..250
FT                   /note="2,5-dichloro-2,5-cyclohexadiene-1,4-diol
FT                   dehydrogenase"
FT                   /id="PRO_0000444941"
FT   ACT_SITE        154
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10001"
FT   BINDING         9..34
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250"
FT   BINDING         141
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   CONFLICT        54
FT                   /note="A -> P (in Ref. 1; AAN64242)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   250 AA;  25600 MW;  5F693F18A9783404 CRC64;
     MSDLSGKTII VTGGGSGIGR ATVELLVASG ANVAVADIND EAGEAVVAAS GGKAAYFRCD
     IAQEEDVKAL VAQTLAAFGG LDGAFNNAAI PQAGLPLAEV SLERFRQSMD INVTGTFLCM
     KYQILAMIER GTKGSIVNTA SVAGVVGVPM HGEYVGAKHA VVGLTRVAAA DYGKHGIRVN
     ALVPGAVRTP MLQRAMDNDA GLEPYLNSIH PIGRFSEPHE QAQAAVWLLS DAASFVTGSC
     LAADGGFTAI