LINC_SPHJU
ID LINC_SPHJU Reviewed; 250 AA.
AC D4YYG1; P50197;
DT 18-JUL-2018, integrated into UniProtKB/Swiss-Prot.
DT 15-JUN-2010, sequence version 1.
DT 03-AUG-2022, entry version 60.
DE RecName: Full=2,5-dichloro-2,5-cyclohexadiene-1,4-diol dehydrogenase {ECO:0000303|PubMed:7515041};
DE Short=2,5-DDOL dehydrogenase {ECO:0000303|PubMed:7515041};
DE EC=1.1.1.- {ECO:0000269|PubMed:7515041};
GN Name=linC {ECO:0000303|PubMed:7515041};
GN OrderedLocusNames=SJA_C1-05590 {ECO:0000312|EMBL:BAI95393.1};
OS Sphingobium japonicum (strain DSM 16413 / CCM 7287 / MTCC 6362 / UT26 /
OS NBRC 101211 / UT26S).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales;
OC Sphingomonadaceae; Sphingobium.
OX NCBI_TaxID=452662;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, PATHWAY,
RP DISRUPTION PHENOTYPE, AND INDUCTION.
RC STRAIN=DSM 16413 / CCM 7287 / MTCC 6362 / UT26 / NBRC 101211 / UT26S;
RX PubMed=7515041; DOI=10.1128/jb.176.11.3117-3125.1994;
RA Nagata Y., Ohtomo R., Miyauchi K., Fukuda M., Yano K., Takagi M.;
RT "Cloning and sequencing of a 2,5-dichloro-2,5-cyclohexadiene-1,4-diol
RT dehydrogenase gene involved in the degradation of gamma-
RT hexachlorocyclohexane in Pseudomonas paucimobilis.";
RL J. Bacteriol. 176:3117-3125(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 16413 / CCM 7287 / MTCC 6362 / UT26 / NBRC 101211 / UT26S;
RX PubMed=20817768; DOI=10.1128/jb.00961-10;
RA Nagata Y., Ohtsubo Y., Endo R., Ichikawa N., Ankai A., Oguchi A., Fukui S.,
RA Fujita N., Tsuda M.;
RT "Complete genome sequence of the representative gamma-
RT hexachlorocyclohexane-degrading bacterium Sphingobium japonicum UT26.";
RL J. Bacteriol. 192:5852-5853(2010).
CC -!- FUNCTION: Catalyzes the dehydrogenation of 2,5-dichloro-2,5-
CC cyclohexadiene-1,4-diol (2,5-DDOL) to 2,5-dichlorohydroquinone (2,5-
CC DCHQ), a step in the degradation of gamma-hexachlorocyclohexane (gamma-
CC HCH or lindane). Has an essential role in this assimilation pathway
CC that allows S.japonicum UT26 to grow on gamma-HCH as the sole source of
CC carbon and energy. {ECO:0000269|PubMed:7515041}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2,5-dichlorocyclohexa-2,5-dien-1,4-diol + NAD(+) = 2,5-
CC dichlorohydroquinone + H(+) + NADH; Xref=Rhea:RHEA:15741,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:27545, ChEBI:CHEBI:28975,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945;
CC Evidence={ECO:0000269|PubMed:7515041};
CC -!- PATHWAY: Xenobiotic degradation; gamma-hexachlorocyclohexane
CC degradation. {ECO:0000269|PubMed:7515041}.
CC -!- INDUCTION: Constitutively expressed. {ECO:0000269|PubMed:7515041}.
CC -!- DISRUPTION PHENOTYPE: Cells lacking this gene lose the ability to grow
CC on gamma-HCH; growth is restored when a plasmid containing the linC
CC gene is introduced. {ECO:0000269|PubMed:7515041}.
CC -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR)
CC family. {ECO:0000305}.
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DR EMBL; D14595; BAA03444.1; -; Genomic_DNA.
DR EMBL; AP010803; BAI95393.1; -; Genomic_DNA.
DR RefSeq; WP_013039118.1; NC_014006.1.
DR AlphaFoldDB; D4YYG1; -.
DR SMR; D4YYG1; -.
DR STRING; 452662.SJA_C1-05590; -.
DR EnsemblBacteria; BAI95393; BAI95393; SJA_C1-05590.
DR KEGG; sjp:SJA_C1-05590; -.
DR eggNOG; COG1028; Bacteria.
DR HOGENOM; CLU_010194_1_0_5; -.
DR OMA; KGVMFGM; -.
DR UniPathway; UPA00689; -.
DR Proteomes; UP000007753; Chromosome 1.
DR GO; GO:0018502; F:2,5-dichloro-2,5-cyclohexadiene-1,4-diol dehydrogenase activity; IEA:RHEA.
DR GO; GO:0009636; P:response to toxic substance; IEA:UniProtKB-KW.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR020904; Sc_DH/Rdtase_CS.
DR InterPro; IPR002347; SDR_fam.
DR PRINTS; PR00081; GDHRDH.
DR PRINTS; PR00080; SDRFAMILY.
DR SUPFAM; SSF51735; SSF51735; 1.
DR PROSITE; PS00061; ADH_SHORT; 1.
PE 1: Evidence at protein level;
KW Detoxification; NAD; Oxidoreductase; Reference proteome.
FT CHAIN 1..250
FT /note="2,5-dichloro-2,5-cyclohexadiene-1,4-diol
FT dehydrogenase"
FT /id="PRO_0000054719"
FT ACT_SITE 154
FT /note="Proton acceptor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10001"
FT BINDING 9..34
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250"
FT BINDING 141
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT CONFLICT 34
FT /note="A -> P (in Ref. 1; BAA03444)"
FT /evidence="ECO:0000305"
FT CONFLICT 49
FT /note="A -> T (in Ref. 1; BAA03444)"
FT /evidence="ECO:0000305"
FT CONFLICT 84
FT /note="A -> S (in Ref. 1; BAA03444)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 250 AA; 25572 MW; 89B24B8AEAC91CA6 CRC64;
MSDLSGKTII VTGGGSGIGR ATVELLVASG ANVAVADIND EAGEAVVAAS GGKAAYFRCD
IAQEEDVKAL VAQTLAAFGG LDGAFNNAAI PQAGLPLAEV SLERFRQSMD INVTGTFLCM
KYQILAMIER GTKGSIVNTA SAAGVVGVPM HGEYVGAKHA VVGLTRVAAA DYGKHGIRVN
ALVPGAVRTP MLQRAMDNDA GLEPYLNSIH PIGRFSEPHE QAQAAVWLLS DAASFVTGSC
LAADGGFTAI
