LINES_DROME
ID LINES_DROME Reviewed; 858 AA.
AC Q9V4Z9;
DT 16-AUG-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 127.
DE RecName: Full=Protein lines;
GN Name=lin {ECO:0000312|FlyBase:FBgn0002552}; ORFNames=CG11770;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1] {ECO:0000305, ECO:0000312|EMBL:AAF76228.1}
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, AND TISSUE
RP SPECIFICITY.
RX PubMed=10837029;
RA Hatini V., Bokor P., Goto-Mandeville R., DiNardo S.;
RT "Tissue- and stage-specific modulation of Wingless signaling by the segment
RT polarity gene lines.";
RL Genes Dev. 14:1364-1376(2000).
RN [2] {ECO:0000312|EMBL:AAF59024.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000269|PubMed:10731132};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3] {ECO:0000305, ECO:0000312|EMBL:AAF59024.1}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4] {ECO:0000312|EMBL:AAX33526.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley {ECO:0000312|EMBL:AAX33526.1}; TISSUE=Embryo;
RA Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M.,
RA Park S., Wan K.H., Yu C., Rubin G.M., Celniker S.E.;
RL Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
RN [5] {ECO:0000305}
RP FUNCTION.
RX PubMed=8620835; DOI=10.1242/dev.122.4.1083;
RA Bokor P., DiNardo S.;
RT "The roles of hedgehog, wingless and lines in patterning the dorsal
RT epidermis in Drosophila.";
RL Development 122:1083-1092(1996).
RN [6] {ECO:0000305}
RP FUNCTION.
RX PubMed=9477325; DOI=10.1242/dev.125.7.1269;
RA Castelli-Gair J.;
RT "The lines gene of Drosophila is required for specific functions of the
RT Abdominal-B HOX protein.";
RL Development 125:1269-1274(1998).
RN [7] {ECO:0000305}
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=11784087; DOI=10.1006/dbio.2001.0483;
RA Iwaki D.D., Johansen K.A., Singer J.B., Lengyel J.A.;
RT "Drumstick, bowl, and lines are required for patterning and cell
RT rearrangement in the Drosophila embryonic hindgut.";
RL Dev. Biol. 240:611-626(2001).
RN [8] {ECO:0000305}
RP INTERACTION WITH DRM.
RX PubMed=12117814; DOI=10.1242/dev.129.15.3645;
RA Green R.B., Hatini V., Johansen K.A., Liu X.-J., Lengyel J.A.;
RT "Drumstick is a zinc finger protein that antagonizes Lines to control
RT patterning and morphogenesis of the Drosophila hindgut.";
RL Development 129:3645-3656(2002).
RN [9] {ECO:0000305}
RP FUNCTION.
RX PubMed=14568103; DOI=10.1016/j.mod.2003.08.001;
RA Johansen K.A., Green R.B., Iwaki D.D., Hernandez J.B., Lengyel J.A.;
RT "The Drm-Bowl-Lin relief-of-repression hierarchy controls fore- and hindgut
RT patterning and morphogenesis.";
RL Mech. Dev. 120:1139-1151(2003).
CC -!- FUNCTION: Has a dual role as a segment polarity protein and as a
CC modulator of the Abd-B protein. Required for Abd-B to activate the
CC transcription of genes (including ems, cut and sal) that are involved
CC in posterior spiracle morphogenesis. Also required for Abd-B to form an
CC eighth abdominal denticle belt. Acts in a hierarchy downstream of drm
CC and upstream of bowl during foregut and hindgut patterning and
CC morphogenesis. Involved in cell rearrangement during elongation of the
CC embryonic hindgut. Required to regulate expression of embryonic hindgut
CC patterning genes in order to establish the large intestine and at least
CC some rectum, and to repress small intestine fate. Required for late
CC wingless (wg)-dependent cell fate specification in the dorsal embryonic
CC epidermis. Acts in concert with wg to regulate expression of wg itself
CC and also to regulate wg-target genes. May have a role in ventral
CC epidermal patterning, independent of wg signaling.
CC {ECO:0000269|PubMed:10837029, ECO:0000269|PubMed:11784087,
CC ECO:0000269|PubMed:14568103, ECO:0000269|PubMed:8620835,
CC ECO:0000269|PubMed:9477325}.
CC -!- SUBUNIT: Interacts with drm. {ECO:0000269|PubMed:12117814}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:10837029}. Nucleus
CC {ECO:0000269|PubMed:10837029}. Note=Cytoplasmic in the half of the
CC parasegment, posterior to the engrailed (en) domain, and nuclear in the
CC half of the parasegment anterior to the en domain.
CC -!- TISSUE SPECIFICITY: Expressed throughout the embryo, including the
CC hindgut, posterior midgut and embryonic epidermis.
CC {ECO:0000269|PubMed:10837029, ECO:0000269|PubMed:11784087}.
CC -!- SIMILARITY: Belongs to the protein lines family. {ECO:0000305}.
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DR EMBL; AF272358; AAF76228.1; -; mRNA.
DR EMBL; AE013599; AAF59024.1; -; Genomic_DNA.
DR EMBL; BT021378; AAX33526.1; -; mRNA.
DR RefSeq; NP_524815.1; NM_080076.3.
DR AlphaFoldDB; Q9V4Z9; -.
DR SMR; Q9V4Z9; -.
DR BioGRID; 69592; 7.
DR IntAct; Q9V4Z9; 2.
DR STRING; 7227.FBpp0087732; -.
DR PaxDb; Q9V4Z9; -.
DR PRIDE; Q9V4Z9; -.
DR DNASU; 45325; -.
DR EnsemblMetazoa; FBtr0088651; FBpp0087732; FBgn0002552.
DR GeneID; 45325; -.
DR KEGG; dme:Dmel_CG11770; -.
DR UCSC; CG11770-RA; d. melanogaster.
DR CTD; 45325; -.
DR FlyBase; FBgn0002552; lin.
DR VEuPathDB; VectorBase:FBgn0002552; -.
DR eggNOG; ENOG502QT6F; Eukaryota.
DR GeneTree; ENSGT00390000001790; -.
DR HOGENOM; CLU_009492_0_0_1; -.
DR InParanoid; Q9V4Z9; -.
DR OMA; WENIISV; -.
DR OrthoDB; 390872at2759; -.
DR PhylomeDB; Q9V4Z9; -.
DR SignaLink; Q9V4Z9; -.
DR BioGRID-ORCS; 45325; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 45325; -.
DR PRO; PR:Q9V4Z9; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0002552; Expressed in egg cell and 32 other tissues.
DR ExpressionAtlas; Q9V4Z9; baseline and differential.
DR Genevisible; Q9V4Z9; DM.
DR GO; GO:0005737; C:cytoplasm; IDA:FlyBase.
DR GO; GO:0005634; C:nucleus; IDA:FlyBase.
DR GO; GO:0048617; P:embryonic foregut morphogenesis; IMP:UniProtKB.
DR GO; GO:0048619; P:embryonic hindgut morphogenesis; IMP:UniProtKB.
DR GO; GO:0009880; P:embryonic pattern specification; IMP:UniProtKB.
DR GO; GO:0008544; P:epidermis development; IMP:FlyBase.
DR GO; GO:0007440; P:foregut morphogenesis; IMP:FlyBase.
DR GO; GO:0007442; P:hindgut morphogenesis; IMP:FlyBase.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IMP:UniProtKB.
DR GO; GO:0009946; P:proximal/distal axis specification; IMP:FlyBase.
DR GO; GO:0007367; P:segment polarity determination; IEA:UniProtKB-KW.
DR GO; GO:0035277; P:spiracle morphogenesis, open tracheal system; IMP:UniProtKB.
DR GO; GO:0035220; P:wing disc development; IMP:FlyBase.
DR GO; GO:0016055; P:Wnt signaling pathway; IEA:UniProtKB-KW.
DR InterPro; IPR029415; Lines_C.
DR InterPro; IPR032794; LINES_N.
DR InterPro; IPR024875; Protein_Lines.
DR PANTHER; PTHR16057; PTHR16057; 1.
DR Pfam; PF14695; LINES_C; 1.
DR Pfam; PF14694; LINES_N; 1.
PE 1: Evidence at protein level;
KW Activator; Cytoplasm; Developmental protein; Nucleus; Reference proteome;
KW Repressor; Segmentation polarity protein; Transcription;
KW Transcription regulation; Wnt signaling pathway.
FT CHAIN 1..858
FT /note="Protein lines"
FT /id="PRO_0000084437"
FT REGION 1..102
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 18..42
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 68..102
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 858 AA; 96292 MW; 95030ED5FE096139 CRC64;
MDTSSAAGSG AGTGDVCPCS TVATSTTSAS NEQPQTKRQK IEQQIKVGSK ALPPQPPHPP
DILDLDANSN SHLCSSLSSS SSHSLSTPST ANNSPTTTPC SSRAASYAQL LHNILPQNGD
TDLHSQPESA HDEVDRAAKL PNLLTLDTTN GNGSHASSRS NCLQATTATS IDDMLEFEQS
LTRQCLCGVS ERTLRKPFQS HYSQDTNGQK RIAYLREWPT NKLLQFLSNL QLLFDIYLKQ
NAKGFICTRI MDVCDALIRN DHKLIDEIIV LAGYENSYVQ FLAGRVLAAF LVIAKQELND
EWLQKIVDQL FNFEQLDQAA VQKIHFSLDI IKRIVEWKDM EIHPLDDDWM ASANSTSAAS
SVVPLSTEAS VSYMHFPVQE QPLATNYFAL QFREDTEGER ETGQEAPDNR DRHRRHFGED
MNVAYEPHPA PQSTTMPSGC HVVTLTDSES FDTTHLKCIT IQKLEHKWPT LVKNMSELMA
PTHQDAAEHC VLNFLQLWEN IISVKANLSI DETRPYYAQL DKFELLLSHS LSCTVYKQML
CLFNEALCYG STLALQDMLP EETCKLAHQI VCHVRGFRIL ESLPRRQPDN MVSLIGYNGK
PMVYANGTIT LAHAAQSGDS EEDGAPLDLI EMDKTLLQKM VLLVLKSIAV TVKEIRSDSS
DSSIDSTDYD AFQDMMLIER SIRDVLSKLE TFIKQTLEFH PECHFSKILI HLFDDQDDHL
IEAMVCTLDV TSGISFRNNA FPELVAMLNP VYTFLEFLKM TSNSSDLLLD LLVSNETCFL
LYLLRLLKYI RMNWTMFVHS CHTFGMGSAM LDEAMGVLIR LRLQISRLVS RQLYPYDISP
VLRLLESCES LYEGNELS
