ID   LINR3_BPT4              Reviewed;          82 AA.
AC   P17309;
DT   01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1993, sequence version 2.
DT   12-AUG-2020, entry version 66.
DE   RecName: Full=Lysis inhibition accessory protein {ECO:0000255|HAMAP-Rule:MF_04112};
DE   AltName: Full=Protein rIII {ECO:0000303|PubMed:27381920};
GN   Name=rIII; Synonyms=31.-1;
OS   Enterobacteria phage T4 (Bacteriophage T4).
OC   Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC   Caudovirales; Myoviridae; Tevenvirinae; Tequatrovirus.
OX   NCBI_TaxID=10665;
OH   NCBI_TaxID=562; Escherichia coli.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=D;
RX   PubMed=2362813; DOI=10.1093/nar/18.12.3635;
RA   Prilipov A.G., Mesyanzhinov V.V., Aebi U., Kellenberger E.;
RT   "Cloning and sequencing of bacteriophage T4 genes between map positions
RT   128.3-130.3.";
RL   Nucleic Acids Res. 18:3635-3635(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2377483; DOI=10.1093/nar/18.14.4280;
RA   Raudonikiene A., Nivinskas R.;
RT   "Nucleotide sequence of bacteriophage T4 gene 31 region.";
RL   Nucleic Acids Res. 18:4280-4280(1990).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND CHARACTERIZATION.
RX   PubMed=1587487; DOI=10.1016/0378-1119(92)90711-w;
RA   Raudonikiene A., Nivinskas R.;
RT   "Gene rIII is the nearest downstream neighbour of bacteriophage T4 gene
RT   31.";
RL   Gene 114:85-90(1992).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND MUTAGENESIS OF HIS-42 AND LYS-82.
RX   PubMed=8244025; DOI=10.1016/0378-1119(93)90187-8;
RA   Raudonikiene A., Nivinskas R.;
RT   "The sequences of gene rIII of bacteriophage T4 and its mutants.";
RL   Gene 134:135-136(1993).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=12626685; DOI=10.1128/mmbr.67.1.86-156.2003;
RA   Miller E.S., Kutter E., Mosig G., Arisaka F., Kunisawa T., Ruger W.;
RT   "Bacteriophage T4 genome.";
RL   Microbiol. Mol. Biol. Rev. 67:86-156(2003).
RN   [6]
RP   INTERACTION WITH LYSIS HOLIN, MUTAGENESIS OF HIS-42, SUBUNIT, AND FUNCTION.
RX   PubMed=27381920; DOI=10.1128/jb.00294-16;
RA   Chen Y., Young R.;
RT   "The last r locus unveiled: T4 RIII is a cytoplasmic antiholin.";
RL   J. Bacteriol. 198:2448-2457(2016).
CC   -!- FUNCTION: Probably binds to the cytoplasmic part of the holin during
CC       lysis inhibition and stabilizes the holin-antiholin complex thereby
CC       resulting in a robust block of the hole formation. {ECO:0000255|HAMAP-
CC       Rule:MF_04112, ECO:0000269|PubMed:27381920}.
CC   -!- SUBUNIT: Homooligomer. Interacts with holin (via N-terminus).
CC       {ECO:0000255|HAMAP-Rule:MF_04112, ECO:0000269|PubMed:27381920}.
CC   -!- SIMILARITY: Belongs to the T4likevirus lysis inhibition accessory
CC       protein rIII family. {ECO:0000255|HAMAP-Rule:MF_04112}.
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DR   EMBL; X17657; CAA35652.1; -; Genomic_DNA.
DR   EMBL; M37882; AAA32507.1; -; Genomic_DNA.
DR   EMBL; X54536; CAA38406.1; -; Genomic_DNA.
DR   EMBL; AF158101; AAD42650.1; -; Genomic_DNA.
DR   PIR; JT0765; JT0765.
DR   RefSeq; NP_049824.1; NC_000866.4.
DR   GeneID; 1258761; -.
DR   KEGG; vg:1258761; -.
DR   Proteomes; UP000009087; Genome.
DR   GO; GO:1901218; P:negative regulation of holin activity; IDA:UniProtKB.
DR   HAMAP; MF_04112; Linr3_BPT4; 1.
DR   InterPro; IPR034688; Linr3.
PE   1: Evidence at protein level;
KW   Reference proteome.
FT   CHAIN           1..82
FT                   /note="Lysis inhibition accessory protein"
FT                   /id="PRO_0000165072"
FT   SITE            42
FT                   /note="Interaction with holin"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04112,
FT                   ECO:0000269|PubMed:27381920"
FT   MUTAGEN         42
FT                   /note="H->R: In r67; r-type (rapid lysis) plaque
FT                   morphology."
FT                   /evidence="ECO:0000269|PubMed:8244025"
FT   MUTAGEN         82
FT                   /note="K->E: In rES40 and rBB9; r-type (rapid lysis) plaque
FT                   morphology. Loss of interaction with holin. No effect on
FT                   homooligomerization."
FT                   /evidence="ECO:0000269|PubMed:8244025"
FT   CONFLICT        64..82
FT                   /note="KPLASAARKAVRHFVVTLK -> NLWLLLHEKQFVTLW (in Ref. 1;
FT                   CAA35652)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   82 AA;  9325 MW;  CFE8DF45AB9CC2CD CRC64;
     MIKQLQHALE LQRNAWNNGH ENYGASIDVE AEALEILRYF KHLNPAQTAL AAELQEKDEL
     KYAKPLASAA RKAVRHFVVT LK