LINS_ARATH
ID LINS_ARATH Reviewed; 569 AA.
AC Q84UV0; Q9SYA3;
DT 02-SEP-2008, integrated into UniProtKB/Swiss-Prot.
DT 11-JAN-2011, sequence version 2.
DT 03-AUG-2022, entry version 117.
DE RecName: Full=S-(+)-linalool synthase, chloroplastic;
DE EC=4.2.3.25 {ECO:0000269|PubMed:12566586};
DE AltName: Full=Terpenoid synthase 14;
DE Short=AtTPS14;
DE Flags: Precursor;
GN Name=TPS14; OrderedLocusNames=At1g61680; ORFNames=T13M11.3;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, AND TISSUE
RP SPECIFICITY.
RC STRAIN=cv. Landsberg erecta;
RX PubMed=12566586; DOI=10.1105/tpc.007989;
RA Chen F., Tholl D., D'Auria J.C., Farooq A., Pichersky E., Gershenzon J.;
RT "Biosynthesis and emission of terpenoid volatiles from Arabidopsis
RT flowers.";
RL Plant Cell 15:481-494(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=12207221; DOI=10.1007/s00438-002-0709-y;
RA Aubourg S., Lecharny A., Bohlmann J.;
RT "Genomic analysis of the terpenoid synthase (AtTPS) gene family of
RT Arabidopsis thaliana.";
RL Mol. Genet. Genomics 267:730-745(2002).
RN [5]
RP GENE FAMILY.
RX PubMed=12777052; DOI=10.1023/a:1023005504702;
RA Lange B.M., Ghassemian M.;
RT "Genome organization in Arabidopsis thaliana: a survey for genes involved
RT in isoprenoid and chlorophyll metabolism.";
RL Plant Mol. Biol. 51:925-948(2003).
CC -!- FUNCTION: Involved in monoterpene (C10) biosynthesis. The major product
CC is (S)-linalool. {ECO:0000269|PubMed:12566586}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(2E)-geranyl diphosphate + H2O = (S)-linalool + diphosphate;
CC Xref=Rhea:RHEA:24116, ChEBI:CHEBI:98, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:33019, ChEBI:CHEBI:58057; EC=4.2.3.25;
CC Evidence={ECO:0000269|PubMed:12566586};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:24117;
CC Evidence={ECO:0000305|PubMed:12566586};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250};
CC Note=Binds 3 Mg(2+) or Mn(2+) ions per subunit. {ECO:0000250};
CC -!- PATHWAY: Secondary metabolite biosynthesis; terpenoid biosynthesis.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=Q84UV0-1; Sequence=Displayed;
CC -!- TISSUE SPECIFICITY: Predominantly expressed in flowers but also in
CC stems and siliques. {ECO:0000269|PubMed:12566586}.
CC -!- DOMAIN: The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for
CC the catalytic activity, presumably through binding to Mg(2+).
CC -!- SIMILARITY: Belongs to the terpene synthase family. Tpsb subfamily.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAD21414.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AF497485; AAO85533.1; -; mRNA.
DR EMBL; AC005882; AAD21414.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE33873.1; -; Genomic_DNA.
DR PIR; C96642; C96642.
DR RefSeq; NP_176361.2; NM_104850.2. [Q84UV0-1]
DR AlphaFoldDB; Q84UV0; -.
DR SMR; Q84UV0; -.
DR STRING; 3702.AT1G61680.1; -.
DR iPTMnet; Q84UV0; -.
DR PaxDb; Q84UV0; -.
DR PRIDE; Q84UV0; -.
DR ProteomicsDB; 238606; -. [Q84UV0-1]
DR EnsemblPlants; AT1G61680.1; AT1G61680.1; AT1G61680. [Q84UV0-1]
DR GeneID; 842465; -.
DR Gramene; AT1G61680.1; AT1G61680.1; AT1G61680. [Q84UV0-1]
DR KEGG; ath:AT1G61680; -.
DR Araport; AT1G61680; -.
DR TAIR; locus:2195768; AT1G61680.
DR eggNOG; ENOG502QTGK; Eukaryota.
DR InParanoid; Q84UV0; -.
DR OMA; VHLLFML; -.
DR OrthoDB; 449049at2759; -.
DR PhylomeDB; Q84UV0; -.
DR BioCyc; ARA:AT1G61680-MON; -.
DR UniPathway; UPA00213; -.
DR PRO; PR:Q84UV0; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q84UV0; baseline and differential.
DR Genevisible; Q84UV0; AT.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR GO; GO:0034007; F:S-linalool synthase activity; IDA:TAIR.
DR GO; GO:0010333; F:terpene synthase activity; IEA:InterPro.
DR GO; GO:0043693; P:monoterpene biosynthetic process; IDA:TAIR.
DR GO; GO:0016114; P:terpenoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 1.10.600.10; -; 1.
DR Gene3D; 1.50.10.130; -; 1.
DR InterPro; IPR008949; Isoprenoid_synthase_dom_sf.
DR InterPro; IPR034741; Terpene_cyclase-like_1_C.
DR InterPro; IPR001906; Terpene_synth_N.
DR InterPro; IPR036965; Terpene_synth_N_sf.
DR InterPro; IPR005630; Terpene_synthase_metal-bd.
DR InterPro; IPR008930; Terpenoid_cyclase/PrenylTrfase.
DR Pfam; PF01397; Terpene_synth; 1.
DR Pfam; PF03936; Terpene_synth_C; 1.
DR SFLD; SFLDG01019; Terpene_Cyclase_Like_1_C_Termi; 1.
DR SUPFAM; SSF48239; SSF48239; 1.
DR SUPFAM; SSF48576; SSF48576; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Chloroplast; Lyase; Magnesium; Manganese;
KW Metal-binding; Plastid; Reference proteome; Transit peptide.
FT TRANSIT 1..39
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 40..569
FT /note="S-(+)-linalool synthase, chloroplastic"
FT /id="PRO_0000348420"
FT MOTIF 331..335
FT /note="DDXXD motif"
FT BINDING 331
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 331
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 335
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 335
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 475
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="3"
FT /evidence="ECO:0000250"
FT BINDING 479
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="3"
FT /evidence="ECO:0000250"
FT BINDING 483
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="3"
FT /evidence="ECO:0000250"
FT CONFLICT 71
FT /note="L -> S (in Ref. 1; AAO85533)"
FT /evidence="ECO:0000305"
FT CONFLICT 105
FT /note="I -> T (in Ref. 1; AAO85533)"
FT /evidence="ECO:0000305"
FT CONFLICT 112
FT /note="E -> G (in Ref. 1; AAO85533)"
FT /evidence="ECO:0000305"
FT CONFLICT 151
FT /note="I -> S (in Ref. 1; AAO85533)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 569 AA; 65401 MW; D2FEA0C870FBA051 CRC64;
MALIATKISS RSCFVSAYPN NSPTFLISKF PNTVDSLSPA NTAKRSILRN VHASVSNPSK
QFHNKTSLEY LHELNIKKIK NILSANVDVP SENLEMIDVI QSLGIDLHFR QEIEQTLHMI
YKEGLQFNGD LHEIALRFRL LRQEGHYVQE IIFKNILDKK GGFKDVVKND VKGLTELFEA
SELRVEGEET LDGAREFTYS RLNELCSGRE SHQKQEIMKS LAQPRHKTVR GLTSKRFTSM
IKIAGQEDPE WLQSLLRVAE IDSIRLKSLT QGEMSQTFKW WTELGLEKDV EKARSQPLKW
HTWSMKILQD PTLTEQRLDL TKPISLVYVI DDIFDVYGEL EELTIFTRVV ERWDHKGLKT
LPKYMRVCFE ALDMITTEIS MKIYKSHGWN PTYALRQSWA SLCKAFLVEA KWFNSGYLPT
TEEYMKNGVV SSGVHLVMLH AYILLGEELT KEKVELIESN PGIVSSAATI LRLWDDLGSA
KDENQDGTDG SYVECYLNEY KGSTVDEART HVAQKISRAW KRLNRECLNP CPFSRSFSKA
CLNIARTVPL MYSYDDDQRL PDEYLKSLM
