ID   LINX_SPHJU              Reviewed;         250 AA.
AC   D4Z260; P50198;
DT   18-JUL-2018, integrated into UniProtKB/Swiss-Prot.
DT   15-JUN-2010, sequence version 1.
DT   03-AUG-2022, entry version 60.
DE   RecName: Full=2,5-dichloro-2,5-cyclohexadiene-1,4-diol dehydrogenase LinX {ECO:0000303|PubMed:7515041};
DE            Short=2,5-DDOL dehydrogenase {ECO:0000303|PubMed:7515041};
DE            EC=1.1.1.- {ECO:0000269|PubMed:7515041};
GN   Name=linX {ECO:0000303|PubMed:7515041};
GN   OrderedLocusNames=SJA_C1-18580 {ECO:0000312|EMBL:BAI96692.1};
OS   Sphingobium japonicum (strain DSM 16413 / CCM 7287 / MTCC 6362 / UT26 /
OS   NBRC 101211 / UT26S).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales;
OC   Sphingomonadaceae; Sphingobium.
OX   NCBI_TaxID=452662;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND CATALYTIC ACTIVITY.
RC   STRAIN=DSM 16413 / CCM 7287 / MTCC 6362 / UT26 / NBRC 101211 / UT26S;
RX   PubMed=7515041; DOI=10.1128/jb.176.11.3117-3125.1994;
RA   Nagata Y., Ohtomo R., Miyauchi K., Fukuda M., Yano K., Takagi M.;
RT   "Cloning and sequencing of a 2,5-dichloro-2,5-cyclohexadiene-1,4-diol
RT   dehydrogenase gene involved in the degradation of gamma-
RT   hexachlorocyclohexane in Pseudomonas paucimobilis.";
RL   J. Bacteriol. 176:3117-3125(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 16413 / CCM 7287 / MTCC 6362 / UT26 / NBRC 101211 / UT26S;
RX   PubMed=20817768; DOI=10.1128/jb.00961-10;
RA   Nagata Y., Ohtsubo Y., Endo R., Ichikawa N., Ankai A., Oguchi A., Fukui S.,
RA   Fujita N., Tsuda M.;
RT   "Complete genome sequence of the representative gamma-
RT   hexachlorocyclohexane-degrading bacterium Sphingobium japonicum UT26.";
RL   J. Bacteriol. 192:5852-5853(2010).
CC   -!- FUNCTION: Catalyzes the degradation of 2,5-dichloro-2,5-cyclohexadiene-
CC       1,4-diol (2,5-DDOL) into 2,5-dichlorohydroquinone (2,5-DCHQ) in vitro.
CC       LinX appears not to be involved in gamma-hexachlorocyclohexane (gamma-
CC       HCH) degradation pathway, in contrast to LinC which has the same
CC       enzymatic activity. {ECO:0000269|PubMed:7515041}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2,5-dichlorocyclohexa-2,5-dien-1,4-diol + NAD(+) = 2,5-
CC         dichlorohydroquinone + H(+) + NADH; Xref=Rhea:RHEA:15741,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:27545, ChEBI:CHEBI:28975,
CC         ChEBI:CHEBI:57540, ChEBI:CHEBI:57945;
CC         Evidence={ECO:0000269|PubMed:7515041};
CC   -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR)
CC       family. {ECO:0000305}.
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DR   EMBL; D23722; BAA04939.1; -; Genomic_DNA.
DR   EMBL; AP010803; BAI96692.1; -; Genomic_DNA.
DR   RefSeq; WP_007682029.1; NC_014006.1.
DR   AlphaFoldDB; D4Z260; -.
DR   SMR; D4Z260; -.
DR   STRING; 452662.SJA_C1-18580; -.
DR   EnsemblBacteria; BAI96692; BAI96692; SJA_C1-18580.
DR   KEGG; sjp:SJA_C1-18580; -.
DR   eggNOG; COG1028; Bacteria.
DR   HOGENOM; CLU_010194_1_0_5; -.
DR   OMA; AKDANTM; -.
DR   Proteomes; UP000007753; Chromosome 1.
DR   GO; GO:0018502; F:2,5-dichloro-2,5-cyclohexadiene-1,4-diol dehydrogenase activity; IEA:RHEA.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR020904; Sc_DH/Rdtase_CS.
DR   InterPro; IPR002347; SDR_fam.
DR   PRINTS; PR00081; GDHRDH.
DR   PRINTS; PR00080; SDRFAMILY.
DR   SUPFAM; SSF51735; SSF51735; 1.
DR   PROSITE; PS00061; ADH_SHORT; 1.
PE   1: Evidence at protein level;
KW   NAD; Oxidoreductase; Reference proteome.
FT   CHAIN           1..250
FT                   /note="2,5-dichloro-2,5-cyclohexadiene-1,4-diol
FT                   dehydrogenase LinX"
FT                   /id="PRO_0000054720"
FT   ACT_SITE        156
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10001"
FT   BINDING         9..34
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250"
FT   BINDING         143
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   CONFLICT        80..81
FT                   /note="EA -> DG (in Ref. 1; BAA04939)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   250 AA;  25520 MW;  0B4CB3A6E8F5C9DD CRC64;
     MANRLAGKVA LITGGASGLG AAQAKRFAEE GAKVVIGDLN EEMAKGVVAE IRAAGGDALF
     IRLDVTDAAS WNNAIAAAVE AFGGLTTLSN TAGIIHPGGF EEESIEGWNK MVAVNQTAIF
     LGIKAAIPEL VKSGNGSIIN ISSLIGMFPT AGNASYCATK AAVRIMSKAA ALEFVDRGVR
     VNTIVPGGMN TPITANVPPD VLKQQTSQIP MGKLGDPIDI ANGALFLASD EAKYITGVDL
     PIDGGWSVGV