ID   LIP14_BORBU             Reviewed;         121 AA.
AC   P0DV83;
DT   25-MAY-2022, integrated into UniProtKB/Swiss-Prot.
DT   25-MAY-2022, sequence version 1.
DT   03-AUG-2022, entry version 2.
DE   RecName: Full=Outer membrane lipoprotein BBA14 {ECO:0000303|PubMed:35215098, ECO:0000305|PubMed:28069820};
DE   Flags: Precursor;
GN   OrderedLocusNames=BB_A14; ORFNames=BB_RS05175;
OS   Borreliella burgdorferi (strain ATCC 35210 / DSM 4680 / CIP 102532 / B31)
OS   (Borrelia burgdorferi).
OG   Plasmid lp54.
OC   Bacteria; Spirochaetes; Spirochaetales; Borreliaceae; Borreliella.
OX   NCBI_TaxID=224326;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 35210 / DSM 4680 / CIP 102532 / B31;
RX   PubMed=9403685; DOI=10.1038/37551;
RA   Fraser C.M., Casjens S., Huang W.M., Sutton G.G., Clayton R.A.,
RA   Lathigra R., White O., Ketchum K.A., Dodson R.J., Hickey E.K., Gwinn M.L.,
RA   Dougherty B.A., Tomb J.-F., Fleischmann R.D., Richardson D.L.,
RA   Peterson J.D., Kerlavage A.R., Quackenbush J., Salzberg S.L., Hanson M.,
RA   van Vugt R., Palmer N., Adams M.D., Gocayne J.D., Weidman J.F.,
RA   Utterback T.R., Watthey L., McDonald L.A., Artiach P., Bowman C.,
RA   Garland S.A., Fujii C., Cotton M.D., Horst K., Roberts K.M., Hatch B.,
RA   Smith H.O., Venter J.C.;
RT   "Genomic sequence of a Lyme disease spirochaete, Borrelia burgdorferi.";
RL   Nature 390:580-586(1997).
RN   [2]
RP   IMMUNOGENICITY.
RC   STRAIN=ATCC 35210 / DSM 4680 / CIP 102532 / B31;
RX   PubMed=18976702; DOI=10.1016/j.micpath.2008.09.006;
RA   Xu Y., Bruno J.F., Luft B.J.;
RT   "Profiling the humoral immune response to Borrelia burgdorferi infection
RT   with protein microarrays.";
RL   Microb. Pathog. 45:403-407(2008).
RN   [3]
RP   SUBCELLULAR LOCATION, AND IDENTIFICATION BY MASS SPECTROMETRY.
RC   STRAIN=ATCC 35210 / DSM 4680 / CIP 102532 / B31;
RX   PubMed=28069820; DOI=10.1128/jb.00658-16;
RA   Dowdell A.S., Murphy M.D., Azodi C., Swanson S.K., Florens L., Chen S.,
RA   Zueckert W.R.;
RT   "Comprehensive Spatial Analysis of the Borrelia burgdorferi Lipoproteome
RT   Reveals a Compartmentalization Bias toward the Bacterial Surface.";
RL   J. Bacteriol. 199:e00658-e00658(2017).
RN   [4]
RP   X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF 26-121, AND PUTATIVE FUNCTION.
RC   STRAIN=ATCC 35210 / DSM 4680 / CIP 102532 / B31;
RX   PubMed=35215098; DOI=10.3390/pathogens11020154;
RA   Akopjana I., Brangulis K.;
RT   "Structural Analysis of the Outer Membrane Lipoprotein BBA14 (OrfD) and the
RT   Corresponding Paralogous Gene Family 143 (PFam143) from Borrelia
RT   burgdorferi.";
RL   Pathogens 11:0-0(2022).
CC   -!- FUNCTION: Outer membrane lipoprotein that could act as a component of a
CC       potential toxin-antitoxin system in B.burgdorferi which could serve as
CC       a plasmid stabilization mechanism in a growing bacterial population.
CC       {ECO:0000303|PubMed:35215098}.
CC   -!- SUBCELLULAR LOCATION: Cell outer membrane {ECO:0000255|PROSITE-
CC       ProRule:PRU00303, ECO:0000269|PubMed:28069820}; Lipid-anchor
CC       {ECO:0000255|PROSITE-ProRule:PRU00303, ECO:0000269|PubMed:28069820};
CC       Extracellular side {ECO:0000269|PubMed:28069820}.
CC   -!- MISCELLANEOUS: Is a highly immunogenic protein recognized by sera from
CC       Lyme disease patients. {ECO:0000269|PubMed:18976702}.
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DR   EMBL; AE000790; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   RefSeq; WP_010258119.1; NC_001857.2.
DR   PDB; 7QDV; X-ray; 1.90 A; A=26-121.
DR   PDBsum; 7QDV; -.
DR   Proteomes; UP000001807; Plasmid lp54.
DR   GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR   InterPro; IPR004248; Borrelia_plasmid_OrfD.
DR   Pfam; PF02999; Borrelia_orfD; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Cell outer membrane; Lipoprotein; Membrane; Palmitate;
KW   Plasmid; Reference proteome; Signal.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   CHAIN           20..121
FT                   /note="Outer membrane lipoprotein BBA14"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT                   /id="PRO_5002963029"
FT   LIPID           20
FT                   /note="N-palmitoyl cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   LIPID           20
FT                   /note="S-diacylglycerol cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
SQ   SEQUENCE   121 AA;  13950 MW;  ED898C47ECA8D7EB CRC64;
     MQIKNFPFLF LLNSLIIFSC STIASLPEEP SSPQESTLKA LSLYEAHLSS YIMYLQTFLV
     KTKQKVNNKN YPEFTLFDTS KLKKDQTLKS IKTNIAALKN HIDKIKPIAM QIYKKYSKNI
     P