ID   LIP4_CANAL              Reviewed;         459 AA.
AC   Q9P8W1; A0A1D8PQF5; Q59RY6;
DT   21-NOV-2003, integrated into UniProtKB/Swiss-Prot.
DT   10-MAY-2017, sequence version 2.
DT   25-MAY-2022, entry version 96.
DE   RecName: Full=Lipase 4;
DE            EC=3.1.1.3;
DE   Flags: Precursor;
GN   Name=LIP4; OrderedLocusNames=CAALFM_C604490WA; ORFNames=CaO19.2133;
OS   Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Debaryomycetaceae; Candida/Lodderomyces clade; Candida.
OX   NCBI_TaxID=237561;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND SUBCELLULAR LOCATION.
RC   STRAIN=1161;
RX   PubMed=11131027; DOI=10.1007/s002030000218;
RA   Hube B., Stehr F., Bossenz M., Mazur A., Kretschmar M., Schaefer W.;
RT   "Secreted lipases of Candida albicans: cloning, characterisation and
RT   expression analysis of a new gene family with at least ten members.";
RL   Arch. Microbiol. 174:362-374(2000).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=SC5314 / ATCC MYA-2876;
RX   PubMed=15123810; DOI=10.1073/pnas.0401648101;
RA   Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B.,
RA   Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W.,
RA   Scherer S.;
RT   "The diploid genome sequence of Candida albicans.";
RL   Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=SC5314 / ATCC MYA-2876;
RX   PubMed=17419877; DOI=10.1186/gb-2007-8-4-r52;
RA   van het Hoog M., Rast T.J., Martchenko M., Grindle S., Dignard D.,
RA   Hogues H., Cuomo C., Berriman M., Scherer S., Magee B.B., Whiteway M.,
RA   Chibana H., Nantel A., Magee P.T.;
RT   "Assembly of the Candida albicans genome into sixteen supercontigs aligned
RT   on the eight chromosomes.";
RL   Genome Biol. 8:RESEARCH52.1-RESEARCH52.12(2007).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC   STRAIN=SC5314 / ATCC MYA-2876;
RX   PubMed=24025428; DOI=10.1186/gb-2013-14-9-r97;
RA   Muzzey D., Schwartz K., Weissman J.S., Sherlock G.;
RT   "Assembly of a phased diploid Candida albicans genome facilitates allele-
RT   specific measurements and provides a simple model for repeat and indel
RT   structure.";
RL   Genome Biol. 14:RESEARCH97.1-RESEARCH97.14(2013).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a triacylglycerol + H2O = a diacylglycerol + a fatty acid +
CC         H(+); Xref=Rhea:RHEA:12044, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:17855, ChEBI:CHEBI:18035, ChEBI:CHEBI:28868; EC=3.1.1.3;
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:11131027}.
CC   -!- SIMILARITY: Belongs to the AB hydrolase superfamily. Lipase family.
CC       {ECO:0000305}.
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DR   EMBL; AF191317; AAF69521.1; -; Genomic_DNA.
DR   EMBL; CP017628; AOW30365.1; -; Genomic_DNA.
DR   RefSeq; XP_712408.2; XM_707315.2.
DR   AlphaFoldDB; Q9P8W1; -.
DR   SMR; Q9P8W1; -.
DR   ESTHER; canal-LIP4; Fungal-Bact_LIP.
DR   GeneID; 3645987; -.
DR   KEGG; cal:CAALFM_C604490WA; -.
DR   CGD; CAL0000184506; LIP4.
DR   VEuPathDB; FungiDB:C6_04490W_A; -.
DR   eggNOG; ENOG502SI7U; Eukaryota.
DR   HOGENOM; CLU_029538_5_0_1; -.
DR   InParanoid; Q9P8W1; -.
DR   OrthoDB; 838782at2759; -.
DR   Proteomes; UP000000559; Chromosome 6.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0016298; F:lipase activity; IDA:CGD.
DR   GO; GO:0004806; F:triglyceride lipase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016042; P:lipid catabolic process; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.1820; -; 1.
DR   InterPro; IPR029058; AB_hydrolase.
DR   InterPro; IPR005152; Lipase_secreted.
DR   PANTHER; PTHR34853; PTHR34853; 1.
DR   Pfam; PF03583; LIP; 1.
DR   PIRSF; PIRSF029171; Esterase_LipA; 1.
DR   SUPFAM; SSF53474; SSF53474; 1.
PE   3: Inferred from homology;
KW   Glycoprotein; Hydrolase; Lipid degradation; Lipid metabolism;
KW   Reference proteome; Secreted; Signal.
FT   SIGNAL          1..14
FT                   /evidence="ECO:0000255"
FT   CHAIN           15..459
FT                   /note="Lipase 4"
FT                   /id="PRO_0000017823"
FT   ACT_SITE        194
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        339
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000250"
FT   CARBOHYD        229
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        266
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        41
FT                   /note="E -> D (in Ref. 1; AAF69521)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        185
FT                   /note="D -> N (in Ref. 1; AAF69521)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        456
FT                   /note="P -> S (in Ref. 1; AAF69521)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   459 AA;  49580 MW;  D38354ADB6FE1F5D CRC64;
     MLFLLFLLVA PIYAGLILPT KPSNDPFYNA PAGFEKAAVG EILQSRKTPK PITGVFVPVK
     IQNSWQLLVR SEDSFGNPNV IVTTVMEPFN ADPSKLASYQ VFEDSAKADC APSYALQFGS
     DVTTIATQVE TYLLAPLLDQ GYYVVSPDYE GPKSTFTVGK QSGQAVLNSI RAALKSGKIT
     NLAEDAKVVM WGYSGGSLAS GWAAALQPNY APELGGNLLG AALGGFVTNI TATAEATDGT
     VFAGIMANAL GGVANEYPEF KQILQNDTDK QSVFDQFDNH CLADGVINYI GKHFLSGTNK
     IFKSGWNILK NPTISKIVED NGLVYQKQLV PKIPILIYHG AIDQIVPIVN VKKTYQNWCD
     AGIASLEFSE DATNGHITET IVGAPVALTW IINRFNGKQT VSGCQHVKRT SNFEYPNIPP
     SILNYFKAAL NILIQKGLGP DIQKDQVNPD GLKKIPILV