LIP8_CANAL
ID LIP8_CANAL Reviewed; 460 AA.
AC Q9P8V9; A0A1D8PRC4; Q59PM6;
DT 21-NOV-2003, integrated into UniProtKB/Swiss-Prot.
DT 10-MAY-2017, sequence version 3.
DT 03-AUG-2022, entry version 91.
DE RecName: Full=Lipase 8;
DE EC=3.1.1.3;
DE Flags: Precursor;
GN Name=LIP8; OrderedLocusNames=CAALFM_C703300CA; ORFNames=CaO19.8925;
OS Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Debaryomycetaceae; Candida/Lodderomyces clade; Candida.
OX NCBI_TaxID=237561;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND SUBCELLULAR LOCATION.
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=11131027; DOI=10.1007/s002030000218;
RA Hube B., Stehr F., Bossenz M., Mazur A., Kretschmar M., Schaefer W.;
RT "Secreted lipases of Candida albicans: cloning, characterisation and
RT expression analysis of a new gene family with at least ten members.";
RL Arch. Microbiol. 174:362-374(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=15123810; DOI=10.1073/pnas.0401648101;
RA Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B.,
RA Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W.,
RA Scherer S.;
RT "The diploid genome sequence of Candida albicans.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=17419877; DOI=10.1186/gb-2007-8-4-r52;
RA van het Hoog M., Rast T.J., Martchenko M., Grindle S., Dignard D.,
RA Hogues H., Cuomo C., Berriman M., Scherer S., Magee B.B., Whiteway M.,
RA Chibana H., Nantel A., Magee P.T.;
RT "Assembly of the Candida albicans genome into sixteen supercontigs aligned
RT on the eight chromosomes.";
RL Genome Biol. 8:RESEARCH52.1-RESEARCH52.12(2007).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=24025428; DOI=10.1186/gb-2013-14-9-r97;
RA Muzzey D., Schwartz K., Weissman J.S., Sherlock G.;
RT "Assembly of a phased diploid Candida albicans genome facilitates allele-
RT specific measurements and provides a simple model for repeat and indel
RT structure.";
RL Genome Biol. 14:RESEARCH97.1-RESEARCH97.14(2013).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a triacylglycerol + H2O = a diacylglycerol + a fatty acid +
CC H(+); Xref=Rhea:RHEA:12044, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:17855, ChEBI:CHEBI:18035, ChEBI:CHEBI:28868; EC=3.1.1.3;
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:11131027}.
CC -!- SIMILARITY: Belongs to the AB hydrolase superfamily. Lipase family.
CC {ECO:0000305}.
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DR EMBL; AF191321; AAF69523.2; -; Genomic_DNA.
DR EMBL; CP017629; AOW30674.1; -; Genomic_DNA.
DR RefSeq; XP_711685.2; XM_706593.2.
DR AlphaFoldDB; Q9P8V9; -.
DR SMR; Q9P8V9; -.
DR ESTHER; canal-LIP8; Fungal-Bact_LIP.
DR PRIDE; Q9P8V9; -.
DR GeneID; 3646727; -.
DR KEGG; cal:CAALFM_C703300CA; -.
DR CGD; CAL0000193439; LIP8.
DR VEuPathDB; FungiDB:C7_03300C_A; -.
DR eggNOG; ENOG502SI7U; Eukaryota.
DR HOGENOM; CLU_029538_5_0_1; -.
DR InParanoid; Q9P8V9; -.
DR OrthoDB; 806580at2759; -.
DR Proteomes; UP000000559; Chromosome 7.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0004806; F:triglyceride lipase activity; IEA:UniProtKB-EC.
DR GO; GO:0016042; P:lipid catabolic process; IEA:UniProtKB-KW.
DR Gene3D; 3.40.50.1820; -; 1.
DR InterPro; IPR029058; AB_hydrolase.
DR InterPro; IPR005152; Lipase_secreted.
DR PANTHER; PTHR34853; PTHR34853; 1.
DR Pfam; PF03583; LIP; 1.
DR PIRSF; PIRSF029171; Esterase_LipA; 1.
DR SUPFAM; SSF53474; SSF53474; 1.
PE 3: Inferred from homology;
KW Glycoprotein; Hydrolase; Lipid degradation; Lipid metabolism;
KW Reference proteome; Secreted; Signal.
FT SIGNAL 1..14
FT /evidence="ECO:0000255"
FT CHAIN 15..460
FT /note="Lipase 8"
FT /id="PRO_0000017827"
FT ACT_SITE 194
FT /note="Charge relay system"
FT /evidence="ECO:0000250"
FT ACT_SITE 339
FT /note="Charge relay system"
FT /evidence="ECO:0000250"
FT CARBOHYD 229
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 291
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 417
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 19
FT /note="L -> P (in Ref. 1; AAF69523)"
FT /evidence="ECO:0000305"
FT CONFLICT 24
FT /note="N -> S (in Ref. 1; AAF69523)"
FT /evidence="ECO:0000305"
FT CONFLICT 60
FT /note="E -> K (in Ref. 1; AAF69523)"
FT /evidence="ECO:0000305"
FT CONFLICT 87
FT /note="Q -> E (in Ref. 1; AAF69523)"
FT /evidence="ECO:0000305"
FT CONFLICT 89
FT /note="F -> V (in Ref. 1; AAF69523)"
FT /evidence="ECO:0000305"
FT CONFLICT 96
FT /note="V -> I (in Ref. 1; AAF69523)"
FT /evidence="ECO:0000305"
FT CONFLICT 105
FT /note="S -> A (in Ref. 1; AAF69523)"
FT /evidence="ECO:0000305"
FT CONFLICT 108
FT /note="G -> A (in Ref. 1; AAF69523)"
FT /evidence="ECO:0000305"
FT CONFLICT 123..126
FT /note="STLA -> TTFV (in Ref. 1; AAF69523)"
FT /evidence="ECO:0000305"
FT CONFLICT 131..134
FT /note="TFLL -> MYLM (in Ref. 1; AAF69523)"
FT /evidence="ECO:0000305"
FT CONFLICT 139
FT /note="E -> D (in Ref. 1; AAF69523)"
FT /evidence="ECO:0000305"
FT CONFLICT 173
FT /note="A -> T (in Ref. 1; AAF69523)"
FT /evidence="ECO:0000305"
FT CONFLICT 177
FT /note="G -> S (in Ref. 1; AAF69523)"
FT /evidence="ECO:0000305"
FT CONFLICT 184
FT /note="D -> E (in Ref. 1; AAF69523)"
FT /evidence="ECO:0000305"
FT CONFLICT 215..217
FT /note="GGN -> SSS (in Ref. 1; AAF69523)"
FT /evidence="ECO:0000305"
FT CONFLICT 312
FT /note="S -> P (in Ref. 1; AAF69523)"
FT /evidence="ECO:0000305"
FT CONFLICT 319
FT /note="K -> E (in Ref. 1; AAF69523)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 460 AA; 49731 MW; C9C48CFDDEFBC577 CRC64;
MLFLLFLLIT PIYAGLIFLT KPSNDPFYNP PKGFEKAAVG DILQSRETPK SITGRFAPLE
IQNSWQLLVR SEDSFGNPNA IVTTVIQPFN ADPSKVASYQ VFEDSAKGDC APSYALQFGS
DLSTLATQAE TFLLAPLLEQ GYYVVSPDYE GPKSTFTIGK QSGQAVLNSI RAALKSGKIT
NIKDDAKVVM WGYSGGSLAS GWAAALQPSY APELGGNLLG AALGGFVTNI TATAQAADGT
VFAGIVANAL GGVANEYPEF KSILQSDTDK KSVFDEFDSH CLADGVIDYI NTSFLTGDNK
IFKTGWDILK SSTIAKIVKD NGLVYQKQLV PKIPIFVYHG SIDQIVPIVN VKKTYQNWCE
GGISSLEFAE DGTNGHLTET VVGAPAALTW IIDRFNGKQT VSGCQHDKRL SNFQYPNISS
SILKYFKVAL DTMMSNGLGS DIQKDKITPD DLRKFLLGGW
