LIPC_BACSU
ID LIPC_BACSU Reviewed; 213 AA.
AC P42969;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 1.
DT 03-AUG-2022, entry version 118.
DE RecName: Full=Spore germination lipase LipC;
DE EC=3.-.-.-;
GN Name=lipC; Synonyms=ycsK; OrderedLocusNames=BSU04110;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RX PubMed=8574415; DOI=10.1099/13500872-141-12-3241;
RA Akagawa E., Kurita K., Sugawara T., Nakamura K., Kasahara Y., Ogasawara N.,
RA Yamane K.;
RT "Determination of a 17,484 bp nucleotide sequence around the 39 degrees
RT region of the Bacillus subtilis chromosome and similarity analysis of the
RT products of putative ORFs.";
RL Microbiology 141:3241-3245(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RX PubMed=8969502; DOI=10.1099/13500872-142-11-3047;
RA Yamane K., Kumano M., Kurita K.;
RT "The 25 degrees-36 degrees region of the Bacillus subtilis chromosome:
RT determination of the sequence of a 146 kb segment and identification of 113
RT genes.";
RL Microbiology 142:3047-3056(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [4]
RP FUNCTION, SUBCELLULAR LOCATION, AND EXPRESSION.
RC STRAIN=168;
RX PubMed=17220230; DOI=10.1128/jb.01527-06;
RA Masayama A., Kuwana R., Takamatsu H., Hemmi H., Yoshimura T., Watabe K.,
RA Moriyama R.;
RT "A novel lipolytic enzyme, YcsK (LipC), located in the spore coat of
RT Bacillus subtilis, is involved in spore germination.";
RL J. Bacteriol. 189:2369-2375(2007).
CC -!- FUNCTION: Lipase involved in spore germination.
CC {ECO:0000269|PubMed:17220230}.
CC -!- SUBCELLULAR LOCATION: Spore coat {ECO:0000269|PubMed:17220230}.
CC -!- DEVELOPMENTAL STAGE: Transcribed from stage t4 of sporulation.
CC Expressed in the mother cell compartment at the late stage of
CC sporulation and localizes around forespores in a CotE, SafA and SpoIVD-
CC dependent manner.
CC -!- INDUCTION: Transcribed under the control of SigK and positively
CC regulated by GerE.
CC -!- SIMILARITY: Belongs to the 'GDSL' lipolytic enzyme family.
CC {ECO:0000305}.
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DR EMBL; D38161; BAA07363.1; -; Genomic_DNA.
DR EMBL; D50453; BAA09041.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB12218.1; -; Genomic_DNA.
DR PIR; I39900; I39900.
DR RefSeq; NP_388292.1; NC_000964.3.
DR RefSeq; WP_003234415.1; NZ_JNCM01000031.1.
DR AlphaFoldDB; P42969; -.
DR SMR; P42969; -.
DR STRING; 224308.BSU04110; -.
DR PaxDb; P42969; -.
DR PRIDE; P42969; -.
DR EnsemblBacteria; CAB12218; CAB12218; BSU_04110.
DR GeneID; 938249; -.
DR KEGG; bsu:BSU04110; -.
DR PATRIC; fig|224308.179.peg.437; -.
DR eggNOG; COG2755; Bacteria.
DR InParanoid; P42969; -.
DR OMA; LYNPFPG; -.
DR PhylomeDB; P42969; -.
DR BioCyc; BSUB:BSU04110-MON; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0004622; F:lysophospholipase activity; IBA:GO_Central.
DR GO; GO:0016042; P:lipid catabolic process; IDA:CACAO.
DR GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR Gene3D; 3.40.50.1110; -; 1.
DR InterPro; IPR013830; SGNH_hydro.
DR InterPro; IPR036514; SGNH_hydro_sf.
DR Pfam; PF13472; Lipase_GDSL_2; 1.
PE 2: Evidence at transcript level;
KW Hydrolase; Reference proteome; Sporulation.
FT CHAIN 1..213
FT /note="Spore germination lipase LipC"
FT /id="PRO_0000049481"
FT ACT_SITE 11
FT /note="Nucleophile"
FT /evidence="ECO:0000250"
FT ACT_SITE 186
FT /evidence="ECO:0000250"
FT ACT_SITE 189
FT /evidence="ECO:0000250"
FT BINDING 50
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 82
FT /ligand="substrate"
FT /evidence="ECO:0000250"
SQ SEQUENCE 213 AA; 23606 MW; 8A0E5C840D0F9A8E CRC64;
MVLRYTALGD SLTTGRGSGL FSPGFVQRFG DMMEADLKTR TAINIFARSG LNTEEILGLL
SYPYVQKCIR DADMITITGC GNDLIDSVLA YQTSKDETIF SRVSAHCHEN FEKMIAKVAE
IKGENPSPYA IRVFNLYNPF PEIDIAGKWI TSFNSHLETL ASAPHVKIAD AYSIFKGKEQ
EYLSFDGVHP NSKGYQAMAE AVHKLGYKEL SVS
