ID   LIPE_AERHY              Reviewed;         684 AA.
AC   P40600;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1995, sequence version 1.
DT   25-MAY-2022, entry version 66.
DE   RecName: Full=Extracellular lipase {ECO:0000303|PubMed:8368830};
DE            EC=3.1.1.3 {ECO:0000269|PubMed:8368830};
DE   AltName: Full=Triacylglycerol lipase {ECO:0000305};
DE   Flags: Precursor;
GN   Name=lip {ECO:0000303|PubMed:8368830};
OS   Aeromonas hydrophila.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Aeromonadales;
OC   Aeromonadaceae; Aeromonas.
OX   NCBI_TaxID=644;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY,
RP   BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT, AND SUBCELLULAR LOCATION.
RC   STRAIN=H3;
RX   PubMed=8368830; DOI=10.1128/aem.59.8.2411-2417.1993;
RA   Anguita J., Rodriguez Aparicio L.B., Naharro G.;
RT   "Purification, gene cloning, amino acid sequence analysis, and expression
RT   of an extracellular lipase from an Aeromonas hydrophila human isolate.";
RL   Appl. Environ. Microbiol. 59:2411-2417(1993).
CC   -!- FUNCTION: The optimum chain lengths for the acyl moiety is C6 for ester
CC       hydrolysis and C6 and C8 for triacylglycerol hydrolysis.
CC       {ECO:0000269|PubMed:8368830}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a triacylglycerol + H2O = a diacylglycerol + a fatty acid +
CC         H(+); Xref=Rhea:RHEA:12044, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:17855, ChEBI:CHEBI:18035, ChEBI:CHEBI:28868; EC=3.1.1.3;
CC         Evidence={ECO:0000269|PubMed:8368830};
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       pH dependence:
CC         Optimum pH is 7.2. {ECO:0000269|PubMed:8368830};
CC       Temperature dependence:
CC         Optimum temperature is 37 degrees Celsius. Retains 90% of its
CC         activity after 30 minutes at 55 degrees Celsius.
CC         {ECO:0000269|PubMed:8368830};
CC   -!- SUBUNIT: Monomer. {ECO:0000269|PubMed:8368830}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:8368830}.
CC   -!- SIMILARITY: Belongs to the AB hydrolase superfamily. Lipase family.
CC       {ECO:0000305}.
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DR   EMBL; S65123; AAB28083.2; -; Genomic_DNA.
DR   AlphaFoldDB; P40600; -.
DR   ESTHER; aerhy-lipe; Lipase_bact_N_lipase.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0004806; F:triglyceride lipase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016042; P:lipid catabolic process; IEA:UniProtKB-KW.
DR   InterPro; IPR025920; Lipase_bact_N.
DR   Pfam; PF12262; Lipase_bact_N; 1.
DR   PROSITE; PS00120; LIPASE_SER; 1.
PE   1: Evidence at protein level;
KW   Hydrolase; Lipid degradation; Lipid metabolism; Secreted; Signal.
FT   SIGNAL          1..48
FT                   /evidence="ECO:0000255"
FT   CHAIN           49..684
FT                   /note="Extracellular lipase"
FT                   /id="PRO_0000017729"
FT   REGION          321..405
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          462..493
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        387..405
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        462..480
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        568
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10037"
SQ   SEQUENCE   684 AA;  71904 MW;  325418D3936FA428 CRC64;
     MKKKLIYAAV VSALLAGCGG SDDNKGDTSS YLDYLLTGSN AVGPSALAAR AWDGTLKFST
     ETADLSNPVS AMSTLDGWST TQAIQIVPVT SSGITVQAPT TAEFGASVAP LYLLEVTFDS
     TALRPSGVKK VLTYGVDFVV AASAWQAEPG SAQAVEPLPC LANDSGHRTA ERQSRRCLKA
     GSDYGNYKNN AGSNAQEQTI NGLIALQEGL FKAATGIATD HVIFSDWFGT QSGADVLVAV
     KGAAASVLKA DPVTLDAAKL WKQDAWEHQP ARHLYPGRDR PTCLPDPAGC RAVPAAEQKD
     AIATAFGPVL RSTRLLKRPR SIPVPSSCLT SSPHRRPQVP GARPRPSPGT VPSQPVRHRQ
     CAEGVTRSDR RAGGGGRGSG PAGDADCRSD PPERAAGRGE QADWGDAHLR RQAAGRRAEH
     WSLQPAADAG RGAIRADACL RQGCPQHHHG CHHLSARRDL GQRERLRPGA GPDLEDLCRH
     AGGQEGGAGG DRSSAARRAW LRLSGSMDTV TTSDNPTPYL NLSYLTVARD NLKQSVAICW
     ACVWRLAWPT PRAIGTAGSL KVHFLGHSLG ASRVPTCCGR QPDHRQRASG CPVQVRYRWP
     GHAGSHSAAA AELADFGPTI KMGVLTSGSA ELKAGFTAYA PNCTDGGAYL LRQRVPAEPG
     RGHSATAATR CRVQLCGPVG AGFG