ID   LIPM_MYCTU              Reviewed;         431 AA.
AC   Q50681; F2GJ96; I6Y0D6; Q7D7C4;
DT   26-FEB-2020, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 141.
DE   RecName: Full=Probable carboxylic ester hydrolase LipM {ECO:0000305};
DE            EC=3.1.1.- {ECO:0000305};
GN   Name=lipM {ECO:0000303|PubMed:27690385};
GN   OrderedLocusNames=Rv2284 {ECO:0000312|EMBL:CCP45066.1};
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2] {ECO:0007744|PubMed:21969609}
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA   Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA   Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA   Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA   Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT   "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT   mass spectrometry.";
RL   Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
RN   [3]
RP   DEVELOPMENTAL STAGE.
RX   PubMed=27690385; DOI=10.1021/acsinfecdis.6b00135;
RA   Tallman K.R., Levine S.R., Beatty K.E.;
RT   "Small-molecule probes reveal esterases with persistent activity in dormant
RT   and reactivating Mycobacterium tuberculosis.";
RL   ACS Infect. Dis. 2:936-944(2016).
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Multi-pass membrane
CC       protein {ECO:0000255}.
CC   -!- DEVELOPMENTAL STAGE: Remains active in dormant M.tuberculosis.
CC       {ECO:0000269|PubMed:27690385}.
CC   -!- SIMILARITY: Belongs to the 'GDXG' lipolytic enzyme family.
CC       {ECO:0000305}.
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DR   EMBL; AL123456; CCP45066.1; -; Genomic_DNA.
DR   RefSeq; NP_216800.1; NC_000962.3.
DR   RefSeq; WP_003899248.1; NZ_NVQJ01000012.1.
DR   AlphaFoldDB; Q50681; -.
DR   SMR; Q50681; -.
DR   STRING; 83332.Rv2284; -.
DR   ChEMBL; CHEMBL4105753; -.
DR   ESTHER; myctu-Rv2284; Hormone-sensitive_lipase_like.
DR   PaxDb; Q50681; -.
DR   PRIDE; Q50681; -.
DR   DNASU; 887794; -.
DR   GeneID; 45426263; -.
DR   GeneID; 887794; -.
DR   KEGG; mtu:Rv2284; -.
DR   PATRIC; fig|83332.111.peg.2539; -.
DR   TubercuList; Rv2284; -.
DR   eggNOG; COG0657; Bacteria.
DR   OMA; GSANHLD; -.
DR   PhylomeDB; Q50681; -.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0005576; C:extracellular region; HDA:MTBBASE.
DR   GO; GO:0005887; C:integral component of plasma membrane; HDA:MTBBASE.
DR   GO; GO:0005886; C:plasma membrane; HDA:MTBBASE.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   GO; GO:0052562; P:suppression by symbiont of host immune response; IDA:MTBBASE.
DR   Gene3D; 3.40.50.1820; -; 1.
DR   InterPro; IPR029058; AB_hydrolase.
DR   InterPro; IPR013094; AB_hydrolase_3.
DR   Pfam; PF07859; Abhydrolase_3; 1.
DR   SUPFAM; SSF53474; SSF53474; 1.
PE   1: Evidence at protein level;
KW   Hydrolase; Membrane; Reference proteome; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..431
FT                   /note="Probable carboxylic ester hydrolase LipM"
FT                   /id="PRO_0000448852"
FT   TRANSMEM        7..27
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        38..58
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        75..95
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        261
FT                   /evidence="ECO:0000250|UniProtKB:O06350"
FT   ACT_SITE        357
FT                   /evidence="ECO:0000250|UniProtKB:O06350"
FT   ACT_SITE        390
FT                   /evidence="ECO:0000250|UniProtKB:O06350"
SQ   SEQUENCE   431 AA;  46681 MW;  37C366DE80E05F14 CRC64;
     MGAPRLIHVI RQIGALVVAA VTAAATINAY RPLARNGFAS LWSWFIGLVV TEFPLPTLAS
     QLGGLVLTAQ RLTRPVRAVS WLVAAFSALG LLNLSRAGRQ ADAQLTAALD SGLGPDRRTA
     SAGLWRRPAG GGTAKTPGPL RMLRIYRDYA HDGDISYGEY GRANHLDIWR RPDLDLTGTA
     PVLFQIPGGA WTTGNKRGQA HPLMSHLAEL GWICVAINYR HSPRNTWPDH IIDVKRALAW
     VKAHISEYGG DPDFIAITGG SAGGHLSSLA ALTPNDPRFQ PGFEEADTRV QAAVPFYGVY
     DFTRLQDAMH PMMLPLLERM VVKQPRTANM QSYLDASPVT HISADAPPFF VLHGRNDSLV
     PVQQARGFVD QLRQVSKQPV VYAELPFTQH AFDLLGSARA AHTAIAVEQF LAEVYATQHA
     GSEPGPAVAI P