ID   LIRP_LOCMI              Reviewed;         145 AA.
AC   P15131;
DT   01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1992, sequence version 2.
DT   25-MAY-2022, entry version 98.
DE   RecName: Full=LIRP;
DE   AltName: Full=Locusta insulin-related peptide;
DE   Contains:
DE     RecName: Full=LIRP B chain;
DE   Contains:
DE     RecName: Full=5 kDa peptide;
DE     AltName: Full=C chain;
DE   Contains:
DE     RecName: Full=LIRP A chain;
DE   Flags: Precursor;
OS   Locusta migratoria (Migratory locust).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Polyneoptera; Orthoptera; Caelifera; Acrididea; Acridomorpha;
OC   Acridoidea; Acrididae; Oedipodinae; Locusta.
OX   NCBI_TaxID=7004;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Brain;
RX   PubMed=1688797; DOI=10.1111/j.1432-1033.1990.tb15302.x;
RA   Lagueux M., Lwoff L., Meister M., Goltzene F., Hoffmann J.A.;
RT   "cDNAs from neurosecretory cells of brains of Locusta migratoria (Insecta,
RT   Orthoptera) encoding a novel member of the superfamily of insulins.";
RL   Eur. J. Biochem. 187:249-254(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8168530; DOI=10.1111/j.1432-1033.1994.tb18755.x;
RA   Kromer-Metzger E., Lagueux M.;
RT   "Expression of the gene encoding an insulin-related peptide in Locusta
RT   (Insecta, Orthoptera). Evidence for alternative promoter usage.";
RL   Eur. J. Biochem. 221:427-434(1994).
RN   [3]
RP   PROTEIN SEQUENCE OF 67-116.
RC   TISSUE=Corpora cardiaca;
RX   PubMed=2298206; DOI=10.1111/j.1432-1033.1990.tb15301.x;
RA   Hietter H., van Dorsselaer A., Green B., Denoroy L., Hoffmann J.A., Luu B.;
RT   "Isolation and structure elucidation of a novel 5-kDa peptide from
RT   neurohaemal lobes of the corpora cardiaca of Locusta migratoria (Insecta,
RT   Orthoptera).";
RL   Eur. J. Biochem. 187:241-247(1990).
RN   [4]
RP   PROTEIN SEQUENCE OF 34-64 AND 123-143.
RC   TISSUE=Corpora cardiaca;
RX   PubMed=1935945; DOI=10.1111/j.1432-1033.1991.tb16308.x;
RA   Hetru C., Li K.-W., Bulet P., Lagueux M., Hoffmann J.A.;
RT   "Isolation and structural characterization of an insulin-related molecule,
RT   a predominant neuropeptide from Locusta migratoria.";
RL   Eur. J. Biochem. 201:495-499(1991).
CC   -!- SUBUNIT: Heterodimer of a B chain and an A chain linked by two
CC       disulfide bonds.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- SIMILARITY: Belongs to the insulin family. {ECO:0000305}.
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DR   EMBL; X17024; CAA34889.1; -; mRNA.
DR   EMBL; Z29963; CAA82851.1; -; Genomic_DNA.
DR   EMBL; Z29964; CAA82852.1; -; Genomic_DNA.
DR   PIR; S43224; S43224.
DR   AlphaFoldDB; P15131; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0005179; F:hormone activity; IEA:UniProtKB-KW.
DR   InterPro; IPR016179; Insulin-like.
DR   InterPro; IPR036438; Insulin-like_sf.
DR   InterPro; IPR040228; Insulin-relat-peptide_inver.
DR   InterPro; IPR022353; Insulin_CS.
DR   InterPro; IPR022352; Insulin_family.
DR   PANTHER; PTHR13647; PTHR13647; 1.
DR   Pfam; PF00049; Insulin; 1.
DR   PRINTS; PR00276; INSULINFAMLY.
DR   SMART; SM00078; IlGF; 1.
DR   SUPFAM; SSF56994; SSF56994; 1.
DR   PROSITE; PS00262; INSULIN; 1.
PE   1: Evidence at protein level;
KW   Cleavage on pair of basic residues; Direct protein sequencing;
KW   Disulfide bond; Hormone; Secreted; Signal.
FT   SIGNAL          1..19
FT                   /note="Or 22"
FT                   /evidence="ECO:0000255"
FT   PROPEP          20..33
FT                   /evidence="ECO:0000269|PubMed:1935945"
FT                   /id="PRO_0000016058"
FT   PEPTIDE         34..64
FT                   /note="LIRP B chain"
FT                   /id="PRO_0000016059"
FT   PEPTIDE         67..116
FT                   /note="5 kDa peptide"
FT                   /id="PRO_0000016060"
FT   PROPEP          117..122
FT                   /evidence="ECO:0000269|PubMed:1935945"
FT                   /id="PRO_0000016061"
FT   PEPTIDE         123..143
FT                   /note="LIRP A chain"
FT                   /id="PRO_0000016062"
FT   DISULFID        44..129
FT                   /note="Interchain (between B and A chains)"
FT                   /evidence="ECO:0000250"
FT   DISULFID        56..142
FT                   /note="Interchain (between B and A chains)"
FT                   /evidence="ECO:0000250"
FT   DISULFID        128..133
FT                   /evidence="ECO:0000250"
FT   CONFLICT        132
FT                   /note="S -> T (in Ref. 1; CAA34889)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   145 AA;  15774 MW;  79B7FCA0A2ED8C0C CRC64;
     MWKLCLRLLA VLAVCLSTAT QAQSDLFLLS PKRSGAPQPV ARYCGEKLSN ALKLVCRGNY
     NTMFKKASQD VSDSESEDNY WSGQSADEAA EAAAAALPPY PILARPSAGG LLTGAVFRRR
     TRGVFDECCR KSCSISELQT YCGRR