LIS12_NEUCR
ID LIS12_NEUCR Reviewed; 486 AA.
AC Q7S7L4; Q86ZG2;
DT 27-JUN-2006, integrated into UniProtKB/Swiss-Prot.
DT 04-DEC-2007, sequence version 2.
DT 25-MAY-2022, entry version 120.
DE RecName: Full=Nuclear distribution protein nudF-1 {ECO:0000255|HAMAP-Rule:MF_03141};
DE AltName: Full=Lissencephaly-1 homolog 2 {ECO:0000255|HAMAP-Rule:MF_03141};
DE Short=LIS-1 2 {ECO:0000255|HAMAP-Rule:MF_03141};
DE AltName: Full=Nuclear migration protein 2;
DE AltName: Full=nudF homolog 1 {ECO:0000255|HAMAP-Rule:MF_03141};
GN Name=nmp-2;
GN Synonyms=dyn-5, lis1-2 {ECO:0000255|HAMAP-Rule:MF_03141}, mtb4B, nudf-1,
GN pac1-2; ORFNames=B7H23.150, NCU04312;
OS Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 /
OS FGSC 987).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Sordariomycetidae; Sordariales; Sordariaceae; Neurospora.
OX NCBI_TaxID=367110;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX PubMed=12655011; DOI=10.1093/nar/gkg293;
RA Mannhaupt G., Montrone C., Haase D., Mewes H.-W., Aign V., Hoheisel J.D.,
RA Fartmann B., Nyakatura G., Kempken F., Maier J., Schulte U.;
RT "What's in the genome of a filamentous fungus? Analysis of the Neurospora
RT genome sequence.";
RL Nucleic Acids Res. 31:1944-1954(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX PubMed=12712197; DOI=10.1038/nature01554;
RA Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D.,
RA Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B.,
RA Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M.,
RA Qui D., Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M.,
RA Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U.,
RA Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D.,
RA Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S.,
RA Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D.,
RA Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S.,
RA Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A.,
RA DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R.,
RA Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R.,
RA Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I.,
RA Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.;
RT "The genome sequence of the filamentous fungus Neurospora crassa.";
RL Nature 422:859-868(2003).
CC -!- FUNCTION: Positively regulates the activity of the minus-end directed
CC microtubule motor protein dynein. May enhance dynein-mediated
CC microtubule sliding by targeting dynein to the microtubule plus end.
CC Required for nuclear migration during vegetative growth as well as
CC development. Required for retrograde early endosome (EE) transport from
CC the hyphal tip. Required for localization of dynein to the mitotic
CC spindle poles. Recruits additional proteins to the dynein complex at
CC SPBs. {ECO:0000255|HAMAP-Rule:MF_03141}.
CC -!- SUBUNIT: Self-associates. Interacts with ro-11/nde1 and dynein.
CC {ECO:0000255|HAMAP-Rule:MF_03141}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton,
CC spindle pole {ECO:0000255|HAMAP-Rule:MF_03141}. Note=Localizes to the
CC plus ends of microtubules at the hyphal tip and the mitotic spindle
CC poles. {ECO:0000255|HAMAP-Rule:MF_03141}.
CC -!- DOMAIN: Dimerization mediated by the LisH domain may be required to
CC activate dynein. {ECO:0000255|HAMAP-Rule:MF_03141}.
CC -!- SIMILARITY: Belongs to the WD repeat LIS1/nudF family.
CC {ECO:0000255|HAMAP-Rule:MF_03141}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAD71040.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; BX294026; CAD71040.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CM002240; EAA31722.3; -; Genomic_DNA.
DR RefSeq; XP_960958.3; XM_955865.3.
DR AlphaFoldDB; Q7S7L4; -.
DR SMR; Q7S7L4; -.
DR STRING; 5141.EFNCRP00000003934; -.
DR EnsemblFungi; EAA31722; EAA31722; NCU04312.
DR GeneID; 3877099; -.
DR KEGG; ncr:NCU04312; -.
DR VEuPathDB; FungiDB:NCU04312; -.
DR HOGENOM; CLU_000288_57_15_1; -.
DR InParanoid; Q7S7L4; -.
DR Proteomes; UP000001805; Chromosome 2, Linkage Group V.
DR GO; GO:0005881; C:cytoplasmic microtubule; IBA:GO_Central.
DR GO; GO:0000776; C:kinetochore; IBA:GO_Central.
DR GO; GO:0005875; C:microtubule associated complex; IBA:GO_Central.
DR GO; GO:0005635; C:nuclear envelope; IBA:GO_Central.
DR GO; GO:0000922; C:spindle pole; IEA:UniProtKB-SubCell.
DR GO; GO:0070840; F:dynein complex binding; IBA:GO_Central.
DR GO; GO:0051010; F:microtubule plus-end binding; IBA:GO_Central.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0000132; P:establishment of mitotic spindle orientation; IBA:GO_Central.
DR GO; GO:0031023; P:microtubule organizing center organization; IBA:GO_Central.
DR GO; GO:0051012; P:microtubule sliding; IEA:UniProtKB-UniRule.
DR GO; GO:0007097; P:nuclear migration; IBA:GO_Central.
DR GO; GO:0047496; P:vesicle transport along microtubule; IBA:GO_Central.
DR Gene3D; 2.130.10.10; -; 1.
DR HAMAP; MF_03141; lis1; 1.
DR InterPro; IPR017252; Dynein_regulator_LIS1.
DR InterPro; IPR020472; G-protein_beta_WD-40_rep.
DR InterPro; IPR037190; LIS1_N.
DR InterPro; IPR006594; LisH.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR019775; WD40_repeat_CS.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR Pfam; PF00400; WD40; 6.
DR PIRSF; PIRSF037647; Dynein_regulator_Lis1; 1.
DR PRINTS; PR00320; GPROTEINBRPT.
DR SMART; SM00320; WD40; 7.
DR SUPFAM; SSF109925; SSF109925; 1.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS50896; LISH; 1.
DR PROSITE; PS00678; WD_REPEATS_1; 3.
DR PROSITE; PS50082; WD_REPEATS_2; 6.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 3: Inferred from homology;
KW Cell cycle; Cell division; Coiled coil; Cytoplasm; Cytoskeleton;
KW Microtubule; Mitosis; Reference proteome; Repeat; Transport; WD repeat.
FT CHAIN 1..486
FT /note="Nuclear distribution protein nudF-1"
FT /id="PRO_0000240428"
FT DOMAIN 17..49
FT /note="LisH"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_03141"
FT REPEAT 122..163
FT /note="WD 1"
FT REPEAT 165..205
FT /note="WD 2"
FT REPEAT 209..256
FT /note="WD 3"
FT REPEAT 259..298
FT /note="WD 4"
FT REPEAT 321..364
FT /note="WD 5"
FT REPEAT 366..405
FT /note="WD 6"
FT REPEAT 448..486
FT /note="WD 7"
SQ SEQUENCE 486 AA; 52922 MW; 3B9B5CDD5C948F98 CRC64;
MAQASVQIGT PLLTTRQAEE LHKSILAYLA SKNLATTAAT LRDELDLAEE SFDAEKAKKY
EGLLEKKWTS VVRLQKKILD LESRNHLLQS EIDNATPSSL SRRTQDPTNW LPKAPPRYVL
ESHRLPVTCV AFHPVFTSLA SGSEDYTIKI WDWELGELER TIKGHTKAVL DVDFGGPRGG
TLLASCSSDL TIKLWDPSDE YKNIRTLPGH DHIVSSVRFI PSGAAGAPAS GNLLVSASKD
NSLKIWDVTT GYCVKTILGH VDWPRAVCPS HDGRYLLSTG SDKSVRLWDL AGGRDAECRL
VMFGHENYNL CCAFAPPTAY PHLAKLAGLE RAPPPSSSAE FMATGSRDKQ IRLWDGRGNC
IKVLVGHDNW VRGLVFHPGG KYLLSVADDR TMRCWDLSQE GRCVQTLTGV YEGFVSCIRW
APPVVKDADA TAGNGRSEGS SLADPVVAAR RRAATVGSVS ASVQIRCVVA TGSVDGAEGK
VRIFAN
