ID   LIS12_TALMQ             Reviewed;         452 AA.
AC   B6QC06;
DT   08-FEB-2011, integrated into UniProtKB/Swiss-Prot.
DT   16-DEC-2008, sequence version 1.
DT   25-MAY-2022, entry version 69.
DE   RecName: Full=Nuclear distribution protein nudF 2 {ECO:0000255|HAMAP-Rule:MF_03141};
DE   AltName: Full=Lissencephaly-1 homolog 2 {ECO:0000255|HAMAP-Rule:MF_03141};
DE            Short=LIS-1 2 {ECO:0000255|HAMAP-Rule:MF_03141};
GN   Name=nudF-2 {ECO:0000255|HAMAP-Rule:MF_03141};
GN   Synonyms=lis1-2 {ECO:0000255|HAMAP-Rule:MF_03141}; ORFNames=PMAA_075960;
OS   Talaromyces marneffei (strain ATCC 18224 / CBS 334.59 / QM 7333)
OS   (Penicillium marneffei).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Trichocomaceae; Talaromyces;
OC   Talaromyces sect. Talaromyces.
OX   NCBI_TaxID=441960;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 18224 / CBS 334.59 / QM 7333;
RX   PubMed=25676766; DOI=10.1128/genomea.01559-14;
RA   Nierman W.C., Fedorova-Abrams N.D., Andrianopoulos A.;
RT   "Genome sequence of the AIDS-associated pathogen Penicillium marneffei
RT   (ATCC18224) and its near taxonomic relative Talaromyces stipitatus
RT   (ATCC10500).";
RL   Genome Announc. 3:E0155914-E0155914(2015).
CC   -!- FUNCTION: Positively regulates the activity of the minus-end directed
CC       microtubule motor protein dynein. May enhance dynein-mediated
CC       microtubule sliding by targeting dynein to the microtubule plus end.
CC       Required for nuclear migration during vegetative growth as well as
CC       development. Required for retrograde early endosome (EE) transport from
CC       the hyphal tip. Required for localization of dynein to the mitotic
CC       spindle poles. Recruits additional proteins to the dynein complex at
CC       SPBs. {ECO:0000255|HAMAP-Rule:MF_03141}.
CC   -!- SUBUNIT: Self-associates. Interacts with nudE and dynein.
CC       {ECO:0000255|HAMAP-Rule:MF_03141}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton {ECO:0000255|HAMAP-
CC       Rule:MF_03141}. Cytoplasm, cytoskeleton, spindle pole
CC       {ECO:0000255|HAMAP-Rule:MF_03141}. Note=Localizes to the plus ends of
CC       microtubules at the hyphal tip and the mitotic spindle poles.
CC       {ECO:0000255|HAMAP-Rule:MF_03141}.
CC   -!- SIMILARITY: Belongs to the WD repeat LIS1/nudF family.
CC       {ECO:0000255|HAMAP-Rule:MF_03141}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; DS995900; EEA26529.1; -; Genomic_DNA.
DR   RefSeq; XP_002147076.1; XM_002147040.1.
DR   AlphaFoldDB; B6QC06; -.
DR   SMR; B6QC06; -.
DR   STRING; 441960.B6QC06; -.
DR   EnsemblFungi; EEA26529; EEA26529; PMAA_075960.
DR   GeneID; 7024721; -.
DR   KEGG; tmf:PMAA_075960; -.
DR   VEuPathDB; FungiDB:PMAA_075960; -.
DR   HOGENOM; CLU_000288_57_15_1; -.
DR   OrthoDB; 995692at2759; -.
DR   PhylomeDB; B6QC06; -.
DR   Proteomes; UP000001294; Unassembled WGS sequence.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-UniRule.
DR   GO; GO:0005874; C:microtubule; IEA:UniProtKB-KW.
DR   GO; GO:0005875; C:microtubule associated complex; IEA:UniProtKB-UniRule.
DR   GO; GO:0000922; C:spindle pole; IEA:UniProtKB-SubCell.
DR   GO; GO:0070840; F:dynein complex binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR   GO; GO:0000132; P:establishment of mitotic spindle orientation; IEA:UniProtKB-UniRule.
DR   GO; GO:0051012; P:microtubule sliding; IEA:UniProtKB-UniRule.
DR   Gene3D; 2.130.10.10; -; 1.
DR   HAMAP; MF_03141; lis1; 1.
DR   InterPro; IPR017252; Dynein_regulator_LIS1.
DR   InterPro; IPR020472; G-protein_beta_WD-40_rep.
DR   InterPro; IPR037190; LIS1_N.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR   InterPro; IPR001680; WD40_repeat.
DR   InterPro; IPR019775; WD40_repeat_CS.
DR   InterPro; IPR036322; WD40_repeat_dom_sf.
DR   Pfam; PF00400; WD40; 7.
DR   PIRSF; PIRSF037647; Dynein_regulator_Lis1; 1.
DR   PRINTS; PR00320; GPROTEINBRPT.
DR   SMART; SM00320; WD40; 7.
DR   SUPFAM; SSF109925; SSF109925; 1.
DR   SUPFAM; SSF50978; SSF50978; 1.
DR   PROSITE; PS00678; WD_REPEATS_1; 3.
DR   PROSITE; PS50082; WD_REPEATS_2; 5.
DR   PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE   3: Inferred from homology;
KW   Cell cycle; Cell division; Coiled coil; Cytoplasm; Cytoskeleton;
KW   Microtubule; Mitosis; Reference proteome; Repeat; Transport; WD repeat.
FT   CHAIN           1..452
FT                   /note="Nuclear distribution protein nudF 2"
FT                   /id="PRO_0000405091"
FT   REPEAT          123..164
FT                   /note="WD 1"
FT   REPEAT          166..206
FT                   /note="WD 2"
FT   REPEAT          210..250
FT                   /note="WD 3"
FT   REPEAT          253..292
FT                   /note="WD 4"
FT   REPEAT          295..355
FT                   /note="WD 5"
FT   REPEAT          357..396
FT                   /note="WD 6"
FT   REPEAT          401..449
FT                   /note="WD 7"
FT   COILED          76..101
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_03141"
SQ   SEQUENCE   452 AA;  49327 MW;  A276C1772404D7F3 CRC64;
     MSQILTTRQA EELHKSMVAY LSSLEASKST AALREELVLP DTFDDATCKK YAGLLEKKFL
     TCSLKANPSK DNGLSALQIL DLESKVAGLQ AELSSLTLTS RSKGGQDPDN WLPGPASTRT
     FESHRDAITC IAFHPIFTSL ASSSEDCTIK IWDWELGELE RTLKGHIRAV TGLDFGGQKG
     NTLLASCSSD LTVKLWDPSK GYANIRTLSG HDHSVSAVRF LTSTENLLVS AGRDASIRIW
     DVSTGYCVKV LRSSDAWIRD ISSSFDGKWL VAGGRDQAVT VWEVATAEQK SALLGHENYI
     ECCVIAPPTS YEHLATLAGL KKPPAPSSSC EFIATGARDK TIRLWESRGR LIKTLVGHDN
     WVRDLVFHPN GKHLISVADD KTIRCWDLSQ EGKLVKTIDN AHSHFVTCMR WGPSATTDVE
     TTNGVTNKGT TKSKFQCVIA TGSADSCVRV FS