LIS1_CANAL
ID LIS1_CANAL Reviewed; 486 AA.
AC Q5A7Q6; A0A1D8PJ30;
DT 27-JUN-2006, integrated into UniProtKB/Swiss-Prot.
DT 26-APR-2005, sequence version 1.
DT 03-AUG-2022, entry version 110.
DE RecName: Full=Nuclear distribution protein PAC1 {ECO:0000255|HAMAP-Rule:MF_03141};
DE AltName: Full=Lissencephaly-1 homolog {ECO:0000255|HAMAP-Rule:MF_03141};
DE Short=LIS-1 {ECO:0000255|HAMAP-Rule:MF_03141};
DE AltName: Full=nudF homolog {ECO:0000255|HAMAP-Rule:MF_03141};
GN Name=PAC1 {ECO:0000255|HAMAP-Rule:MF_03141};
GN Synonyms=LIS1 {ECO:0000255|HAMAP-Rule:MF_03141};
GN OrderedLocusNames=CAALFM_C300530CA; ORFNames=CaO19.12865, CaO19.5410;
OS Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Debaryomycetaceae; Candida/Lodderomyces clade; Candida.
OX NCBI_TaxID=237561;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=15123810; DOI=10.1073/pnas.0401648101;
RA Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B.,
RA Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W.,
RA Scherer S.;
RT "The diploid genome sequence of Candida albicans.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=17419877; DOI=10.1186/gb-2007-8-4-r52;
RA van het Hoog M., Rast T.J., Martchenko M., Grindle S., Dignard D.,
RA Hogues H., Cuomo C., Berriman M., Scherer S., Magee B.B., Whiteway M.,
RA Chibana H., Nantel A., Magee P.T.;
RT "Assembly of the Candida albicans genome into sixteen supercontigs aligned
RT on the eight chromosomes.";
RL Genome Biol. 8:RESEARCH52.1-RESEARCH52.12(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=24025428; DOI=10.1186/gb-2013-14-9-r97;
RA Muzzey D., Schwartz K., Weissman J.S., Sherlock G.;
RT "Assembly of a phased diploid Candida albicans genome facilitates allele-
RT specific measurements and provides a simple model for repeat and indel
RT structure.";
RL Genome Biol. 14:RESEARCH97.1-RESEARCH97.14(2013).
CC -!- FUNCTION: Positively regulates the activity of the minus-end directed
CC microtubule motor protein dynein. Plays a central role in positioning
CC the mitotic spindle at the bud neck during cell division. Targets
CC cytoplasmic dynein to microtubule plus ends, thereby promoting dynein-
CC mediated microtubule sliding along the bud cortex and consequently the
CC movement of the mitotic spindle to the bud neck. {ECO:0000255|HAMAP-
CC Rule:MF_03141}.
CC -!- SUBUNIT: Self-associates. Interacts with NDL1 and dynein.
CC {ECO:0000255|HAMAP-Rule:MF_03141}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton,
CC spindle pole {ECO:0000255|HAMAP-Rule:MF_03141}. Note=Localizes to the
CC plus ends of microtubules and the mitotic spindle poles.
CC {ECO:0000255|HAMAP-Rule:MF_03141}.
CC -!- SIMILARITY: Belongs to the WD repeat LIS1/nudF family.
CC {ECO:0000255|HAMAP-Rule:MF_03141}.
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DR EMBL; CP017625; AOW28103.1; -; Genomic_DNA.
DR RefSeq; XP_717782.1; XM_712689.1.
DR AlphaFoldDB; Q5A7Q6; -.
DR SMR; Q5A7Q6; -.
DR STRING; 237561.Q5A7Q6; -.
DR GeneID; 3640588; -.
DR KEGG; cal:CAALFM_C300530CA; -.
DR CGD; CAL0000197627; orf19.12865.
DR VEuPathDB; FungiDB:C3_00530C_A; -.
DR eggNOG; KOG0295; Eukaryota.
DR HOGENOM; CLU_000288_57_15_1; -.
DR InParanoid; Q5A7Q6; -.
DR OMA; TQECKCV; -.
DR OrthoDB; 995692at2759; -.
DR PRO; PR:Q5A7Q6; -.
DR Proteomes; UP000000559; Chromosome 3.
DR GO; GO:0005881; C:cytoplasmic microtubule; IBA:GO_Central.
DR GO; GO:0000776; C:kinetochore; IBA:GO_Central.
DR GO; GO:0005875; C:microtubule associated complex; IBA:GO_Central.
DR GO; GO:0005635; C:nuclear envelope; IBA:GO_Central.
DR GO; GO:0000922; C:spindle pole; IEA:UniProtKB-SubCell.
DR GO; GO:0070840; F:dynein complex binding; IBA:GO_Central.
DR GO; GO:0051010; F:microtubule plus-end binding; IBA:GO_Central.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0000132; P:establishment of mitotic spindle orientation; IBA:GO_Central.
DR GO; GO:0031023; P:microtubule organizing center organization; IBA:GO_Central.
DR GO; GO:0051012; P:microtubule sliding; IEA:UniProtKB-UniRule.
DR GO; GO:0007097; P:nuclear migration; IBA:GO_Central.
DR GO; GO:0047496; P:vesicle transport along microtubule; IBA:GO_Central.
DR Gene3D; 2.130.10.10; -; 1.
DR HAMAP; MF_03141; lis1; 1.
DR InterPro; IPR017252; Dynein_regulator_LIS1.
DR InterPro; IPR020472; G-protein_beta_WD-40_rep.
DR InterPro; IPR037190; LIS1_N.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR019775; WD40_repeat_CS.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR Pfam; PF00400; WD40; 5.
DR PIRSF; PIRSF037647; Dynein_regulator_Lis1; 1.
DR PRINTS; PR00320; GPROTEINBRPT.
DR SMART; SM00320; WD40; 7.
DR SUPFAM; SSF109925; SSF109925; 1.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS00678; WD_REPEATS_1; 2.
DR PROSITE; PS50082; WD_REPEATS_2; 5.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 3: Inferred from homology;
KW Cell cycle; Cell division; Coiled coil; Cytoplasm; Cytoskeleton;
KW Microtubule; Mitosis; Reference proteome; Repeat; Transport; WD repeat.
FT CHAIN 1..486
FT /note="Nuclear distribution protein PAC1"
FT /id="PRO_0000240423"
FT REPEAT 119..158
FT /note="WD 1"
FT REPEAT 164..205
FT /note="WD 2"
FT REPEAT 206..246
FT /note="WD 3"
FT REPEAT 249..291
FT /note="WD 4"
FT REPEAT 294..328
FT /note="WD 5"
FT REPEAT 329..368
FT /note="WD 6"
FT REPEAT 389..428
FT /note="WD 7"
FT REPEAT 437..483
FT /note="WD 8"
FT COILED 66..99
FT /evidence="ECO:0000255|HAMAP-Rule:MF_03141"
SQ SEQUENCE 486 AA; 55358 MW; 166A0B449BF8A104 CRC64;
MEKLQILTER QQTELNHAII QYLQPLCQQD NHVLLDQLSK LLNIDQSTQE SNNVEKVDNY
LEKRWSTVLR LQKKIIDLEN EISNLNNIIN STNSDNNGII LSKDKINWIP KGAVKQSYQC
ENIVTTVKLH PNLPLVLNGC NDGNLYIWNI SNDDNTIPEK MIKAHTRAIN KICFTYKKPY
YLATCSSDLT IKIWDEKFNH IRTLNGHEHT VSSIQFSPVD NSILYSVSRD KNIRVWDIFQ
GISLKSFVGH SEWCRDLDII SSDTYGDFVL TCSNDQSARL SHANSGAGVA MIVGHSHVVE
TVKFLPSLQA NKILDEYITK NTEQFPTIPL ELLKDKTYNQ LGFKYCITAS RDNTIKLWLI
PPPTIAPHRP PLPSKYNNSQ SWLIAELKGH SSWVKSLCVH PNGKFIISGS DDKTIKFWDL
SGLLETGYVN VVKTIIGHDG FINDIDFARL KEASDVSEED LLKQVEKRMR CLFISGSADN
SIKLWN
